Key features and details
- Rabbit polyclonal to Separase
- Suitable for: IHC-P, WB, ELISA
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Separase antibody
See all Separase primary antibodies
DescriptionRabbit polyclonal to Separase
Specificityab52158 detects endogenous levels of total SEPARASE protein.
Tested applicationsSuitable for: IHC-P, WB, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic non-phosphopeptide derived from human Separase around the phosphorylation site of serine 801 (N-S-S-P-V).
- human colon carcinoma, extracts from A431 cells
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab52158 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|WB||1/500 - 1/1000. Detects a band of approximately >175 kDa (predicted molecular weight: 233 kDa).|
FunctionCaspase-like protease, which plays a central role in the chromosome segregation by cleaving the SCC1/RAD21 subunit of the cohesin complex at the onset of anaphase. During most of the cell cycle, it is inactivated by different mechanisms.
Sequence similaritiesBelongs to the peptidase C50 family.
modificationsAutocleaves. This function, which is not essential for its protease activity, is unknown.
Phosphorylated by CDK1. There are 8 Ser/Thr phosphorylation sites. Among them, Ser-1126 phosphorylation is the major site, which conducts to the enzyme inactivation.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- Caspase like protein ESPL1 antibody
- Caspase-like protein ESPL1 antibody
- ESP 1 antibody
All lanes : Anti-Separase antibody (ab52158) at 1/500 dilution
Lane 1 : extracts from A431 cells
Lane 2 : extracts from A431 cells, with immunizing peptide
Predicted band size: 233 kDa
Observed band size: 233 kDa
Immunohistochemical analysis of separase expression in paraffin embedded human colon carcinoma tissue using ab52158 (1:50 dilution). Left: sample untreated. Right: sample treated with immunizing peptide (negative control).
ab52158 has been referenced in 1 publication.
- Bacac M et al. Securin and separase modulate membrane traffic by affecting endosomal acidification. Traffic 12:615-26 (2011). PubMed: 21272169