Anti-Separase antibody [XJ11-1B12] (ab16170)
Key features and details
- Mouse monoclonal [XJ11-1B12] to Separase
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Separase antibody [XJ11-1B12]
See all Separase primary antibodies -
Description
Mouse monoclonal [XJ11-1B12] to Separase -
Host species
Mouse -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: MDA-MB-231 whole cell lysate.
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General notes
Separase antibodies can be used as a specific marker for centrosomes of mitotic cells. The staining of separase in centrosomes can be detected from prophase of mitosis up until anaphase.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Primary antibody notes
Separase antibodies can be used as a specific marker for centrosomes of mitotic cells. The staining of separase in centrosomes can be detected from prophase of mitosis up until anaphase. -
Clonality
Monoclonal -
Clone number
XJ11-1B12 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab16170 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
Use a concentration of 10 µg/ml. Detects a band of approximately 170 kDa (predicted molecular weight: 233 kDa).
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Notes |
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WB
Use a concentration of 10 µg/ml. Detects a band of approximately 170 kDa (predicted molecular weight: 233 kDa). |
Target
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Function
Caspase-like protease, which plays a central role in the chromosome segregation by cleaving the SCC1/RAD21 subunit of the cohesin complex at the onset of anaphase. During most of the cell cycle, it is inactivated by different mechanisms. -
Sequence similarities
Belongs to the peptidase C50 family. -
Post-translational
modificationsAutocleaves. This function, which is not essential for its protease activity, is unknown.
Phosphorylated by CDK1. There are 8 Ser/Thr phosphorylation sites. Among them, Ser-1126 phosphorylation is the major site, which conducts to the enzyme inactivation. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 9700 Human
- Omim: 604143 Human
- SwissProt: Q14674 Human
- Unigene: 153479 Human
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Alternative names
- Caspase like protein ESPL1 antibody
- Caspase-like protein ESPL1 antibody
- ESP 1 antibody
see all
Images
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Anti-Separase antibody [XJ11-1B12] (ab16170) at 10 µg/ml + MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 20 µg
Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 233 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa, 70 kDa (possible cleavage fragment). We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
Datasheets and documents
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SDS download
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Datasheet download
References (14)
ab16170 has been referenced in 14 publications.
- Shindo N et al. Prolonged mitosis causes separase deregulation and chromosome nondisjunction. Cell Rep 34:108652 (2021). PubMed: 33472072
- Maier NK et al. Separase cleaves the kinetochore protein Meikin at the meiosis I/II transition. Dev Cell 56:2192-2206.e8 (2021). PubMed: 34331869
- Hsu WH et al. Adducin-1 is essential for spindle pole integrity through its interaction with TPX2. EMBO Rep 19:N/A (2018). PubMed: 29925526
- Karki M et al. Precocious centriole disengagement and centrosome fragmentation induced by mitotic delay. Nat Commun 8:15803 (2017). WB ; Human . PubMed: 28607478
- Seo MY et al. Integrity of the Pericentriolar Material Is Essential for Maintaining Centriole Association during M Phase. PLoS One 10:e0138905 (2015). WB . PubMed: 26407333