Key features and details
- Rabbit polyclonal to SEPSECS
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SEPSECS antibody
DescriptionRabbit polyclonal to SEPSECS
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human SEPSECS aa 5-451.
Database link: Q9HD40
- WB: HEK-293T whole cell lysate. IHC-P: Human kidney tissue. ICC/IF: PC-3 cells.
Previously labelled as Soluble Liver/Pancreas Antigen.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
Our Abpromise guarantee covers the use of ab236956 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/500 - 1/2000.|
|ICC/IF||1/50 - 1/200.|
FunctionConverts O-phosphoseryl-tRNA(Sec) to selenocysteinyl-tRNA(Sec) required for selenoprotein biosynthesis.
Tissue specificityPrimarily expressed in liver, pancreas, kidney and lung. Overexpressed in PHA-stimulated T-cells.
PathwayAminoacyl-tRNA biosynthesis; selenocysteinyl-tRNA(Sec) biosynthesis; selenocysteinyl-tRNA(Sec) from L-seryl-tRNA(Sec) (archaeal/eukaryal route): step 2/2.
Involvement in diseaseDefects in SEPSECS are the cause of pontocerebellar hypoplasia type 2D (PCH2D) [MIM:613811]. PCH2D is a disorder characterized by postnatal onset of progressive atrophy of the cerebrum and cerebellum, microcephaly, profound mental retardation, spasticity, and variable seizures.
Sequence similaritiesBelongs to the SepSecS family.
- Information by UniProt
- DKFZp434B1417 antibody
- Liver pancreas antigen antibody
- Liver-pancreas antigen antibody
Anti-SEPSECS antibody (ab236956) at 1/500 dilution + HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Goat polyclonal to Rabbit IgG at 1/10000 dilution
Paraffin-embedded human kidney tissue stained for SEPSECS with ab236956 at a 1/100 dilution in immunohistochemical analysis.
PC-3 (Human prostate adenocarcinoma cell line) cells stained for SEPSECS (Green) using ab236956 at a 1/100 dilution in ICC/IF. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236956 has not yet been referenced specifically in any publications.