Overview

  • Product name
  • Description
    Goat polyclonal to SEPT4
  • Host species
    Goat
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Hamster, Cow, Dog, Pig, Monkey, Zebrafish, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide: C-

    RNKLTRESGTD

    , corresponding to internal sequence amino acids 425-435 of Human SEPT4 (NP_004565.1).

  • Positive control
    • Human brain (cerebellum) tissue; Human and Mouse heart lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab166788 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 55 kDa).
IHC-P Use a concentration of 3.75 µg/ml.

Target

  • Function
    Filament-forming cytoskeletal GTPase (By similarity). May play a role in cytokinesis (Potential). May play a role in platelet secretion. Isoform ARTS, but not the other isoforms, is required for the induction of cell death mediated by TGF-beta and by other apoptotic stimuli.
  • Tissue specificity
    Widely expressed in adult and fetal tissues with highest expression in adult brain (at protein level), heart, liver and adrenal gland and fetal heart, kidney, liver and lung. Also expressed in colorectal cancers and malignant melanomas. Expressed in platelets.
  • Sequence similarities
    Belongs to the septin family.
  • Cellular localization
    Cytoplasm. Cytoplasm > cytoskeleton. In platelets, found in areas surrounding alpha-granules and Mitochondrion. Nucleus. While predominantly localized in the mitochondria under resting conditions, isoform ARTS translocates into the nucleus after TGF-beta treatment and apoptosis induction.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apoptosis-related protein in the TGF-beta signaling pathway antibody
    • ARTS antibody
    • Bradeion antibody
    • Bradeion beta antibody
    • Brain protein H5 antibody
    • CE5B3 antibody
    • CE5B3 beta antibody
    • Cell division control-related protein 2 antibody
    • Cerebral protein 7 antibody
    • H5 antibody
    • hCDCREL-2 antibody
    • hucep-7 antibody
    • MART antibody
    • Peanut-like protein 2 antibody
    • PNUTL2 antibody
    • SEP 4 antibody
    • SEP4 antibody
    • Sept4 antibody
    • SEPT4_HUMAN antibody
    • Septin M antibody
    • Septin-4 antibody
    see all

Images

  • Anti-SEPT4 antibody (ab166788) at 1 µg/ml + Human heart lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 55 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?



    Primary incubation: 1 hour.
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain (cerebellum) tissue labeling SEPT4 with ab166788 at 3.75 µg/ml.

References

ab166788 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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