Key features and details
- Goat polyclonal to SEPT4
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-SEPT4 antibody
See all SEPT4 primary antibodies
DescriptionGoat polyclonal to SEPT4
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Hamster, Cow, Dog, Pig, Monkey, Zebrafish, Gorilla, Orangutan
- Human brain (cerebellum) tissue; Human and Mouse heart lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab166788 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 55 kDa).|
|IHC-P||Use a concentration of 3.75 µg/ml.|
FunctionFilament-forming cytoskeletal GTPase (By similarity). May play a role in cytokinesis (Potential). May play a role in platelet secretion. Isoform ARTS, but not the other isoforms, is required for the induction of cell death mediated by TGF-beta and by other apoptotic stimuli.
Tissue specificityWidely expressed in adult and fetal tissues with highest expression in adult brain (at protein level), heart, liver and adrenal gland and fetal heart, kidney, liver and lung. Also expressed in colorectal cancers and malignant melanomas. Expressed in platelets.
Sequence similaritiesBelongs to the septin family.
Cellular localizationCytoplasm. Cytoplasm > cytoskeleton. In platelets, found in areas surrounding alpha-granules and Mitochondrion. Nucleus. While predominantly localized in the mitochondria under resting conditions, isoform ARTS translocates into the nucleus after TGF-beta treatment and apoptosis induction.
- Information by UniProt
- Apoptosis-related protein in the TGF-beta signaling pathway antibody
- ARTS antibody
- Bradeion antibody
Anti-SEPT4 antibody (ab166788) at 1 µg/ml + Human heart lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 55 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Primary incubation: 1 hour.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain (cerebellum) tissue labeling SEPT4 with ab166788 at 3.75 µg/ml.
ab166788 has not yet been referenced specifically in any publications.