Overview

  • Product name
    Anti-SEPT4 antibody
    See all SEPT4 primary antibodies
  • Description
    Rabbit polyclonal to SEPT4
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human SEPT4 aa 3-14.
    Sequence:

    KRFLEDTTDDGE


    (Peptide available as ab9652)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Human lung, kidney and spleen tissue lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab9651 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 32 kDa.Can be blocked with Human SEPT4 peptide (ab9652).
IHC-P Use a concentration of 2 µg/ml.

Target

  • Function
    Filament-forming cytoskeletal GTPase (By similarity). May play a role in cytokinesis (Potential). May play a role in platelet secretion. Isoform ARTS, but not the other isoforms, is required for the induction of cell death mediated by TGF-beta and by other apoptotic stimuli.
  • Tissue specificity
    Widely expressed in adult and fetal tissues with highest expression in adult brain (at protein level), heart, liver and adrenal gland and fetal heart, kidney, liver and lung. Also expressed in colorectal cancers and malignant melanomas. Expressed in platelets.
  • Sequence similarities
    Belongs to the septin family.
  • Cellular localization
    Cytoplasm. Cytoplasm > cytoskeleton. In platelets, found in areas surrounding alpha-granules and Mitochondrion. Nucleus. While predominantly localized in the mitochondria under resting conditions, isoform ARTS translocates into the nucleus after TGF-beta treatment and apoptosis induction.
  • Information by UniProt
  • Database links
    • Alternative names
      • Apoptosis-related protein in the TGF-beta signaling pathway antibody
      • ARTS antibody
      • Bradeion antibody
      • Bradeion beta antibody
      • Brain protein H5 antibody
      • CE5B3 antibody
      • CE5B3 beta antibody
      • Cell division control-related protein 2 antibody
      • Cerebral protein 7 antibody
      • H5 antibody
      • hCDCREL-2 antibody
      • hucep-7 antibody
      • MART antibody
      • Peanut-like protein 2 antibody
      • PNUTL2 antibody
      • SEP 4 antibody
      • SEP4 antibody
      • Sept4 antibody
      • SEPT4_HUMAN antibody
      • Septin M antibody
      • Septin-4 antibody
      see all

    Images

    • Western blot - Anti-SEPT4 antibody (ab9651)
      Western blot - Anti-SEPT4 antibody (ab9651)

      Western blot analysis of SEPT4 expression in human lung (lane 1), spleen (lane 2), and kidney (lane 3) tissue lysates with anti-SEPT4 at 2 µg /ml.

      Western blot analysis of SEPT4 expression in human lung (lane 1), spleen (lane 2), and kidney (lane 3) tissue lysates with anti-SEPT4 at 2 µg /ml.

    References

    ab9651 has not yet been referenced specifically in any publications.

    Publishing research using ab9651? Please let us know so that we can cite the reference in this datasheet.

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