Anti-SEPT4 antibody (ab9651)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-SEPT4 antibody
See all SEPT4 primary antibodies -
DescriptionRabbit polyclonal to SEPT4
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
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Species reactivityReacts with: Human
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Immunogen
Synthetic peptide corresponding to Human SEPT4 aa 3-14.
Sequence:KRFLEDTTDDGE
(Peptide available asab9652) -
Positive control
- Human lung, kidney and spleen tissue lysates.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservative: 0.02% Sodium azide
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Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab9651 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 20 µg/ml. | |
| WB | Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 32 kDa.Can be blocked with Human SEPT4 peptide (ab9652). | |
| IHC-P | Use a concentration of 2 µg/ml. |
Target
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FunctionFilament-forming cytoskeletal GTPase (By similarity). May play a role in cytokinesis (Potential). May play a role in platelet secretion. Isoform ARTS, but not the other isoforms, is required for the induction of cell death mediated by TGF-beta and by other apoptotic stimuli.
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Tissue specificityWidely expressed in adult and fetal tissues with highest expression in adult brain (at protein level), heart, liver and adrenal gland and fetal heart, kidney, liver and lung. Also expressed in colorectal cancers and malignant melanomas. Expressed in platelets.
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Sequence similaritiesBelongs to the septin family.
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Cellular localizationCytoplasm. Cytoplasm > cytoskeleton. In platelets, found in areas surrounding alpha-granules and Mitochondrion. Nucleus. While predominantly localized in the mitochondria under resting conditions, isoform ARTS translocates into the nucleus after TGF-beta treatment and apoptosis induction.
- Information by UniProt
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Database links
- Entrez Gene: 5414 Human
- Omim: 603696 Human
- SwissProt: O43236 Human
- Unigene: 287518 Human
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Alternative names
- Apoptosis-related protein in the TGF-beta signaling pathway antibody
- ARTS antibody
- Bradeion antibody
see all
Images
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Western blot - Anti-SEPT4 antibody (ab9651)Western blot analysis of SEPT4 expression in human lung (lane 1), spleen (lane 2), and kidney (lane 3) tissue lysates with anti-SEPT4 at 2 µg /ml.
Western blot analysis of SEPT4 expression in human lung (lane 1), spleen (lane 2), and kidney (lane 3) tissue lysates with anti-SEPT4 at 2 µg /ml.
Protocols
Datasheets and documents
References
ab9651 has not yet been referenced specifically in any publications.
