Anti-SEPT5 antibody (ab233431)
Key features and details
- Rabbit polyclonal to SEPT5
- Suitable for: WB, IHC-P
- Reacts with: Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-SEPT5 antibody
See all SEPT5 primary antibodies -
Description
Rabbit polyclonal to SEPT5 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human, Pig
Predicted to work with: Mouse, Rat, Cow, Cynomolgus monkey -
Immunogen
Recombinant fragment (His-tag) corresponding to Human SEPT5 aa 50-350. Expressed in E.coli.
Database link: Q99719 -
Positive control
- WB: Recombinant human SEPT5 protein; Pig brain and heart lysates; HepG2 cell lysate. IHC-P: Human stomach, liver cancer, brain, prostate, breast cancer and pancreas tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233431 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 43 kDa.
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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Notes |
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WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 43 kDa. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
Target
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Function
Filament-forming cytoskeletal GTPase (By similarity). May play a role in cytokinesis (Potential). May play a role in platelet secretion. -
Tissue specificity
Expressed at high levels in the CNS, as well as in heart and platelets (at protein level). -
Sequence similarities
Belongs to the septin family. -
Post-translational
modificationsIn platelets, phosphorylated in response to thrombin, phorbol-12-myristate-13-acetate and collagen. -
Cellular localization
Cytoplasm. Cytoplasm > cytoskeleton. In platelets, found in areas surrounding alpha-granules. - Information by UniProt
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Database links
- Entrez Gene: 615408 Cow
- Entrez Gene: 5413 Human
- Entrez Gene: 18951 Mouse
- Entrez Gene: 116728 Rat
- Omim: 602724 Human
- SwissProt: Q0VC68 Cow
- SwissProt: Q9BGQ3 Cynomolgus monkey
- SwissProt: Q99719 Human
see all -
Alternative names
- 5 Sep antibody
- CDCrel 1 antibody
- CDCREL antibody
see all
Images
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Anti-SEPT5 antibody (ab233431) at 3 µg/ml + Recombinant human SEPT5 protein
Secondary
HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 43 kDa -
All lanes : Anti-SEPT5 antibody (ab233431) at 3 µg/ml
Lane 1 : Pig brain lysate
Lane 2 : Pig heart lysate
Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Secondary
All lanes : HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 43 kDa -
Formalin-fixed, paraffin-embedded human stomach tissue stained for SEPT5 using ab233431 at 25 μg/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human liver cancer tissue stained for SEPT5 using ab233431 at 25 μg/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human brain tissue stained for SEPT5 using ab233431 at 25 μg/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human prostate tissue stained for SEPT5 using ab233431 at 25 μg/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human breast cancer tissue stained for SEPT5 using ab233431 at 25 μg/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human pancreas tissue stained for SEPT5 using ab233431 at 25 μg/ml in immunohistochemical analysis. DAB staining.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab233431 has not yet been referenced specifically in any publications.