Overview

  • Product name

  • Description

    Rabbit polyclonal to SEPT6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to an internal region of Human SEPT6.

  • Positive control

    • HeLa cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
    Note: PBS is without Mg2+ and Ca2+.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab138036 was affinity-purified from rabbit antiserum by immunogenic peptide affinity-chromatography.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab138036 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 49 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Filament-forming cytoskeletal GTPase. Required for normal organization of the actin cytoskeleton. Involved in cytokinesis. May play a role in HCV RNA replication.
  • Tissue specificity

    Widely expressed.
  • Sequence similarities

    Belongs to the septin family.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Cytoplasm. Cytoplasm > cytoskeleton > spindle. Chromosome > centromere > kinetochore. Cleavage furrow. Midbody. In metaphase cells, localized within the microtubule spindle. At the metaphase plate, in close apposition to the kinetochores of the congressed chromosomes. In cells undergoing cytokinesis, localized to the midbody, the ingressing cleavage furrow, and the central spindle.
  • Information by UniProt
  • Database links

  • Alternative names

    • KIAA0128 antibody
    • Nkrf antibody
    • RCG53214, isoform CRA_d antibody
    • RP5-876A24.2 antibody
    • SEP2 antibody
    • SEPT2 antibody
    • SEPT6 antibody
    • SEPT6/MLL FUSION GENE antibody
    • SEPT6_HUMAN antibody
    • Septin 2 antibody
    • Septin 6 antibody
    • Septin-6 antibody
    see all

Images

  • Anti-SEPT6 antibody (ab138036) at 1/500 dilution + HeLa cell lysate at 30 µg

    Predicted band size: 49 kDa

References

This product has been referenced in:

  • Omrane M  et al. Septin 9 has Two Polybasic Domains Critical to Septin Filament Assembly and Golgi Integrity. iScience 13:138-153 (2019). Read more (PubMed: 30831549) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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