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    sept7-antibody-ab186010.pdf

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Cell Biology Cell Cycle Cell Division Cytokinesis
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Anti-SEPT7 antibody (ab186010)

  • Datasheet
  • SDS
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Western blot - Anti-SEPT7 antibody (ab186010)
  • Immunoprecipitation - Anti-SEPT7 antibody (ab186010)

Key features and details

  • Rabbit polyclonal to SEPT7
  • Suitable for: IP, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human SEPT7 protein (ab158072)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-SEPT7 antibody
    See all SEPT7 primary antibodies
  • Description

    Rabbit polyclonal to SEPT7
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human SEPT7 aa 50-150. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_001779.3
    Database link: Q16181

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • HeLa, 293T, Jurkat, TCMK-1, NIH3T3 whole cell lysates,
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7.0-8.0
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Cytokinesis

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab30128)
    • NIH/3T3 whole cell lysate (ab7179)
    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant Human SEPT7 protein (ab158072)
  • Related Products

    • Recombinant Human SEPT7 protein (ab158072)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab186010 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IP
Human
WB
Human
All applications
Rat
Rabbit
Horse
Chicken
Guinea pig
Cow
Cat
Dog
Pig
Chimpanzee
Rhesus monkey
Gorilla
Chinese hamster
Orangutan
Application Abreviews Notes
IP
Use at 2-10 µg/mg of lysate.
WB (2)
1/2000 - 1/10000. Predicted molecular weight: 51 kDa.
Notes
IP
Use at 2-10 µg/mg of lysate.
WB
1/2000 - 1/10000. Predicted molecular weight: 51 kDa.

Target

  • Function

    Filament-forming cytoskeletal GTPase. Required for normal organization of the actin cytoskeleton. Required for normal progress through mitosis. Involved in cytokinesis. Required for normal association of CENPE with the kinetochore. Plays a role in ciliogenesis and collective cell movements.
  • Tissue specificity

    Widely expressed.
  • Sequence similarities

    Belongs to the septin family.
  • Cellular localization

    Cytoplasm. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle. Cleavage furrow. Midbody. Cytoplasm > cytoskeleton > cilium axoneme. Distributed throughout the cytoplasm in prometaphase cells. Associated with the spindle during metaphase. Associated with the central spindle and at the cleavage furrow in anaphase cells. Detected at the midbody in telophase. Associated with actin stress fibers.
  • Target information above from: UniProt accession Q16181 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 101099705 Cat
    • Entrez Gene: 420741 Chicken
    • Entrez Gene: 463349 Chimpanzee
    • Entrez Gene: 408000 Cow
    • Entrez Gene: 100713931 Guinea pig
    • Entrez Gene: 100070492 Horse
    • Entrez Gene: 989 Human
    • Entrez Gene: 235072 Mouse
    • Entrez Gene: 100173884 Orangutan
    • Entrez Gene: 100523875 Pig
    • Entrez Gene: 100350569 Rabbit
    • Entrez Gene: 64551 Rat
    • Entrez Gene: 677666 Rhesus monkey
    • Omim: 603151 Human
    • SwissProt: Q5R1W1 Chimpanzee
    • SwissProt: Q6Q137 Cow
    • SwissProt: Q16181 Human
    • SwissProt: O55131 Mouse
    • SwissProt: Q5R481 Orangutan
    • SwissProt: Q9WVC0 Rat
    • Unigene: 191346 Human
    • Unigene: 270259 Mouse
    • Unigene: 48701 Rat
    see all
  • Alternative names

    • CDC10 antibody
    • CDC10 protein homolog antibody
    • CDC3 antibody
    • Cell division cycle 10 antibody
    • NBLA02942 antibody
    • SEPT7 antibody
    • SEPT7_HUMAN antibody
    • SEPT7A antibody
    • Septin 7 antibody
    • Septin-7 antibody
    see all

Images

  • Western blot - Anti-SEPT7 antibody (ab186010)
    Western blot - Anti-SEPT7 antibody (ab186010)
    All lanes : Anti-SEPT7 antibody (ab186010) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : 293T cell whole cell lysate
    Lane 3 : Jurkat whole cell lysate
    Lane 4 : TCMK-1 whole cell lysate
    Lane 5 : NIH3T3 whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 51 kDa


    Exposure time: 30 seconds
  • Immunoprecipitation - Anti-SEPT7 antibody (ab186010)
    Immunoprecipitation - Anti-SEPT7 antibody (ab186010)

    Detection of SEPT7 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells was immunoprecipitated using ab186010 at 6 µg/mg (1), a control rabbit anti-SEPT7 antibody (2) and negative control IgG (3). For blotting immunopreciptated SEPT7 ab186010 was used at 1 µg/ml. ECL with an exposure time of 10 seconds was used for detection.

Protocols

  • Western blot protocols
  • Immunoprecipitation protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab186010? Please let us know so that we can cite the reference in this datasheet.

ab186010 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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1-2 of 2 Abreviews

Western blot abreview for Anti-SEPT7 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
10 µg
Specification
HeLa
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

Abcam user community

Verified customer

Submitted Jun 13 2019

Western blot abreview for Anti-SEPT7 antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Gel Running Conditions
Reduced Denaturing (4-12% gradient)
Loading amount
10 µg
Specification
HeLa cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More

Abcam user community

Verified customer

Submitted Nov 07 2018

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