Anti-SEPT7 antibody (ab186010)

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Western blot - Anti-SEPT7 antibody (ab186010)
  • Immunoprecipitation - Anti-SEPT7 antibody (ab186010)

Key features and details

  • Rabbit polyclonal to SEPT7
  • Suitable for: IP, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-SEPT7 antibody
    See all SEPT7 primary antibodies

  • Description

    Rabbit polyclonal to SEPT7

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human SEPT7 aa 50-150. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_001779.3
    Database link: Q16181

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • HeLa, 293T, Jurkat, TCMK-1, NIH3T3 whole cell lysates,

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.00
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7.0-8.0
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab186010 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IP
Human
WB
Human
All applications
Rat
Rabbit
Horse
Chicken
Guinea pig
Cow
Cat
Dog
Pig
Chimpanzee
Rhesus monkey
Gorilla
Chinese hamster
Orangutan
Application Abreviews Notes
IP
Use at 2-10 µg/mg of lysate.
WB (2)
1/2000 - 1/10000. Predicted molecular weight: 51 kDa.
Notes
IP
Use at 2-10 µg/mg of lysate.
WB
1/2000 - 1/10000. Predicted molecular weight: 51 kDa.

Target

Images

  • Western blot - Anti-SEPT7 antibody (ab186010)
    Western blot - Anti-SEPT7 antibody (ab186010)
    All lanes : Anti-SEPT7 antibody (ab186010) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : 293T cell whole cell lysate
    Lane 3 : Jurkat whole cell lysate
    Lane 4 : TCMK-1 whole cell lysate
    Lane 5 : NIH3T3 whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 51 kDa


    Exposure time: 30 seconds
  • Immunoprecipitation - Anti-SEPT7 antibody (ab186010)
    Immunoprecipitation - Anti-SEPT7 antibody (ab186010)

    Detection of SEPT7 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells was immunoprecipitated using ab186010 at 6 µg/mg (1), a control rabbit anti-SEPT7 antibody (2) and negative control IgG (3). For blotting immunopreciptated SEPT7 ab186010 was used at 1 µg/ml. ECL with an exposure time of 10 seconds was used for detection.

References (0)

Publishing research using ab186010? Please let us know so that we can cite the reference in this datasheet.

ab186010 has not yet been referenced specifically in any publications.

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1-2 of 2 Abreviews

Western blot abreview for Anti-SEPT7 antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
10 µg
Specification
HeLa
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

Abcam user community

Verified customer

Submitted Jun 13 2019

Western blot abreview for Anti-SEPT7 antibody

 Good
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Gel Running Conditions
Reduced Denaturing (4-12% gradient)
Loading amount
10 µg
Specification
HeLa cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More

Abcam user community

Verified customer

Submitted Nov 07 2018

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