Lane 1 10ug of HeLa cell lysate loaded on 4-12% bis tris gel run in 1X MOPS buffer blot probed with primary septin 7 antibody ab186010 incubated 1:1000.
lysis buffer 1.5X lammeli buffer 0.15M DTT
Western blot was performed by wet transfer of gel onto nitrocellulose membrane 100V for 2 h 4 degrees.
Blocked in 5% milk PBST for 1 h room temp (1X PBS + 0.1% tween 20)
incubated 24h at 4 degrees in 5% PBST with primary antibody 1:1000
Washed 3X PBST
c IgG anti rabbit HRP 1:5000 (Jackson Immunoscience)
Washed 3x PBST
Washed 1X PBS
Developed using ECL prime kit (GE healthcare)
Abcam user community
Submitted Nov 07 2018
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