Anti-SEPT7 antibody (ab186021)
Key features and details
- Rabbit polyclonal to SEPT7
- Suitable for: IP, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-SEPT7 antibody
See all SEPT7 primary antibodies -
Description
Rabbit polyclonal to SEPT7 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis -
Immunogen
Synthetic peptide within Human SEPT7 aa 350 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q16181 -
Positive control
- WB: HeLa, 293T, Jurkat, TCMK-1 and NIH3T3 whole cell lysates. IP: HeLa cell lysate. IHC-P: Human osteosarcoma and mouse teratoma tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab186021 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at 2-10 µg/mg of lysate.
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WB |
1/2000 - 1/10000. Predicted molecular weight: 51 kDa.
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IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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IP
Use at 2-10 µg/mg of lysate. |
WB
1/2000 - 1/10000. Predicted molecular weight: 51 kDa. |
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Filament-forming cytoskeletal GTPase. Required for normal organization of the actin cytoskeleton. Required for normal progress through mitosis. Involved in cytokinesis. Required for normal association of CENPE with the kinetochore. Plays a role in ciliogenesis and collective cell movements. -
Tissue specificity
Widely expressed. -
Sequence similarities
Belongs to the septin family. -
Cellular localization
Cytoplasm. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle. Cleavage furrow. Midbody. Cytoplasm > cytoskeleton > cilium axoneme. Distributed throughout the cytoplasm in prometaphase cells. Associated with the spindle during metaphase. Associated with the central spindle and at the cleavage furrow in anaphase cells. Detected at the midbody in telophase. Associated with actin stress fibers. - Information by UniProt
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Database links
- Entrez Gene: 420741 Chicken
- Entrez Gene: 463349 Chimpanzee
- Entrez Gene: 408000 Cow
- Entrez Gene: 100713931 Guinea pig
- Entrez Gene: 100070492 Horse
- Entrez Gene: 989 Human
- Entrez Gene: 235072 Mouse
- Entrez Gene: 100173884 Orangutan
see all -
Alternative names
- CDC10 antibody
- CDC10 protein homolog antibody
- CDC3 antibody
see all
Images
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All lanes : Anti-SEPT7 antibody (ab186021) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK-1 whole cell lysate
Lane 5 : NIH3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 51 kDa
Exposure time: 1 second -
ab186021 at 1 µg/ml detecting SEPT7 in HeLa whole cell lysate by WB following IP. ab186021 was used for IP at 6 µg/mg of lysate, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 1 second. -
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human osteosarcoma labeling SEPT7 with ab186021 at a 1/1000 dilution.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse teratoma labeling SEPT7 with ab186021 at a 1/1000 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab186021 has been referenced in 3 publications.
- Barreiro-Alonso A et al. HMGB1 Protein Interactions in Prostate and Ovary Cancer Models Reveal Links to RNA Processing and Ribosome Biogenesis through NuRD, THOC and Septin Complexes. Cancers (Basel) 13:N/A (2021). PubMed: 34572914
- Cui C et al. Relationship between autophagy, apoptosis and endoplasmic reticulum stress induced by melatonin in osteoblasts by septin7 expression. Mol Med Rep 21:2427-2434 (2020). PubMed: 32323792
- Meng X et al. Overexpression of septin-7 inhibits melatonin-induced cell apoptosis in human fetal osteoblastic cells via suppression of endoplasmic reticulum stress. Mol Med Rep 17:4817-4822 (2018). PubMed: 29344665