Key features and details
- Rabbit polyclonal to SEPT7
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SEPT7 antibody
See all SEPT7 primary antibodies
DescriptionRabbit polyclonal to SEPT7
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Xenopus laevis, Chimpanzee, Orangutan
- Human brain cerebellum and kidney tissues; HeLa, HUVEC and MCF7 cell extracts.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.87% Sodium chloride, 50% Glycerol
PBS (without Mg2+, Ca2+)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab211482 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||1/500 - 1/1000. Predicted molecular weight: 51 kDa.|
FunctionFilament-forming cytoskeletal GTPase. Required for normal organization of the actin cytoskeleton. Required for normal progress through mitosis. Involved in cytokinesis. Required for normal association of CENPE with the kinetochore. Plays a role in ciliogenesis and collective cell movements.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the septin family.
Cellular localizationCytoplasm. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle. Cleavage furrow. Midbody. Cytoplasm > cytoskeleton > cilium axoneme. Distributed throughout the cytoplasm in prometaphase cells. Associated with the spindle during metaphase. Associated with the central spindle and at the cleavage furrow in anaphase cells. Detected at the midbody in telophase. Associated with actin stress fibers.
- Information by UniProt
- CDC10 antibody
- CDC10 protein homolog antibody
- CDC3 antibody
All lanes : Anti-SEPT7 antibody (ab211482) at 1/500 dilution
Lane 1 : MCF7 cell extract
Lane 2 : HUVEC cell extract
Lane 3 : HeLa cell extract
Lane 4 : HeLa cell extract with synthesized peptide
Predicted band size: 51 kDa
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue labeling SEPT7 with ab211482 at 10 µg/ml.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain cerebellum tissue labeling SEPT7 with ab211482 at 10 µg/ml.
ab211482 has not yet been referenced specifically in any publications.