Overview

  • Product name

  • Description

    Goat polyclonal to SEPT7
  • Host species

    Goat
  • Specificity

    ab79286 is expected to recognize both reported isoforms (NP_001779.3; NP_001011553.2).
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Cow, Dog, Chimpanzee, Orangutan
  • Immunogen

    Synthetic peptide:

    C-DNNKNKGQLTKSP

    , corresponding to internal sequence amino acids 323-335 of Human SEPT7 (NP_001779.3)

  • Positive control

    • HeLa nuclear cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, 0.5% Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab79286 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab79286 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    Peptide ELISA: Antibody detection limit dilution 1/32000.
    WB: Use at a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 51 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    Images

    • Anti-SEPT7 antibody (ab79286) at 0.5 µg/ml + HeLa nuclear lysate (in RIPA buffer) at 35 µg

      Predicted band size: 51 kDa
      Observed band size: 45 kDa
      why is the actual band size different from the predicted?



      Primary incubation was 1 hour. Detected by chemiluminescence.

    References

    ab79286 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Peritoneal macrophages)
    Permeabilization
    Yes - 0,25% Triton
    Specification
    Peritoneal macrophages
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
    Fixative
    Formaldehyde

    Dr. Manoj B Menon

    Verified customer

    Submitted Apr 24 2015

    Abreviews
    Application
    Western blot
    Loading amount
    10000 cells
    Gel Running Conditions
    Reduced Denaturing (7.5 to 16% gradient)
    Sample
    Mouse Cell lysate - whole cell (Bone Marrow Derived Macrophages)
    Specification
    Bone Marrow Derived Macrophages
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Dr. Manoj B Menon

    Verified customer

    Submitted Apr 01 2015

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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