Recombinant
RabMAb

Recombinant Anti-SEPT7 antibody [EPR12610] (ab175229)

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Overview

  • Product name

    Anti-SEPT7 antibody [EPR12610]
    See all SEPT7 primary antibodies

  • Description

    Rabbit monoclonal [EPR12610] to SEPT7

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human SEPT7. The exact sequence is proprietary.
    Database link: Q16181

  • Positive control

    • Jurkat, HeLa, MCF7 and HepG2 whole cell lysate (ab7900); HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR12610

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab175229 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 51 kDa.
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.

    • Target

    • Function

      Filament-forming cytoskeletal GTPase. Required for normal organization of the actin cytoskeleton. Required for normal progress through mitosis. Involved in cytokinesis. Required for normal association of CENPE with the kinetochore. Plays a role in ciliogenesis and collective cell movements.

    • Tissue specificity

      Widely expressed.

    • Sequence similarities

      Belongs to the septin family.

    • Cellular localization

      Cytoplasm. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle. Cleavage furrow. Midbody. Cytoplasm > cytoskeleton > cilium axoneme. Distributed throughout the cytoplasm in prometaphase cells. Associated with the spindle during metaphase. Associated with the central spindle and at the cleavage furrow in anaphase cells. Detected at the midbody in telophase. Associated with actin stress fibers.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • CDC10 antibody
      • CDC10 protein homolog antibody
      • CDC3 antibody
      • Cell division cycle 10 antibody
      • NBLA02942 antibody
      • SEPT7 antibody
      • SEPT7_HUMAN antibody
      • SEPT7A antibody
      • Septin 7 antibody
      • Septin-7 antibody
      see all

    Images

    • Western blot - Anti-SEPT7 antibody [EPR12610] (ab175229)
      Western blot - Anti-SEPT7 antibody [EPR12610] (ab175229)
      All lanes : Anti-SEPT7 antibody [EPR12610] (ab175229) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : MCF7 cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 51 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-SEPT7 antibody [EPR12610] (ab175229)
      Immunocytochemistry/ Immunofluorescence - Anti-SEPT7 antibody [EPR12610] (ab175229)

      Immunofluorescent analysis of HeLa cells labeling SEPT7 with ab175229 at 1/50 dilution. DAPI nuclear staining (blue).

    References

    This product has been referenced in:

    See all 2 Publications for this product

    Publishing research using ab175229? Please let us know so that we can cite the reference in this datasheet.

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