Product nameAnti-SEPT7 antibody [EPR12610]
See all SEPT7 primary antibodies
DescriptionRabbit monoclonal [EPR12610] to SEPT7
Tested applicationsSuitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment within Human SEPT7. The exact sequence is proprietary.
Database link: Q16181
- Jurkat, HeLa, MCF7 and HepG2 whole cell lysate (ab7900); HeLa cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab175229 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 51 kDa.|
|ICC/IF||1/50 - 1/100.|
FunctionFilament-forming cytoskeletal GTPase. Required for normal organization of the actin cytoskeleton. Required for normal progress through mitosis. Involved in cytokinesis. Required for normal association of CENPE with the kinetochore. Plays a role in ciliogenesis and collective cell movements.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the septin family.
Cellular localizationCytoplasm. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle. Cleavage furrow. Midbody. Cytoplasm > cytoskeleton > cilium axoneme. Distributed throughout the cytoplasm in prometaphase cells. Associated with the spindle during metaphase. Associated with the central spindle and at the cleavage furrow in anaphase cells. Detected at the midbody in telophase. Associated with actin stress fibers.
- Information by UniProt
- CDC10 antibody
- CDC10 protein homolog antibody
- CDC3 antibody
All lanes : Anti-SEPT7 antibody [EPR12610] (ab175229) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 51 kDa
Immunofluorescent analysis of HeLa cells labeling SEPT7 with ab175229 at 1/50 dilution. DAPI nuclear staining (blue).
This product has been referenced in:
- Li R et al. Septin 7 mediates high glucose-induced podocyte apoptosis. Biochem Biophys Res Commun 506:522-528 (2018). Read more (PubMed: 30361092) »
- Sinclair LI & Love S Effect of APOE Genotype on Synaptic Proteins in Earlier Adult Life. J Alzheimers Dis 59:1123-1137 (2017). Read more (PubMed: 28731446) »