Recombinant Anti-SEPT7 antibody [EPR12610] (ab175229)
![Western blot - Anti-SEPT7 antibody [EPR12610] (ab175229)](https://www.abcam.com/ps/products/175/ab175229/Images/ab175229-201578-anti-sept7-antibody-epr12610-western-blot.jpg "Western blot - Anti-SEPT7 antibody [EPR12610] (ab175229)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12610] to SEPT7
- Suitable for: WB, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-SEPT7 antibody [EPR12610]
See all SEPT7 primary antibodies -
Description
Rabbit monoclonal [EPR12610] to SEPT7 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Recombinant fragment within Human SEPT7. The exact sequence is proprietary.
Database link: Q16181 -
Positive control
- Jurkat, HeLa, MCF7 and HepG2 whole cell lysate (ab7900); HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12610 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab175229 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Human
|
| IHC-P |
Mouse
|
| WB |
Human
|
| All applications |
Mouse
Rat
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 51 kDa.
|
|
| ICC/IF |
1/50 - 1/100.
|
| Notes |
|---|
|
WB
1/1000 - 1/5000. Predicted molecular weight: 51 kDa. |
|
ICC/IF
1/50 - 1/100. |
Target
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Function
Filament-forming cytoskeletal GTPase. Required for normal organization of the actin cytoskeleton. Required for normal progress through mitosis. Involved in cytokinesis. Required for normal association of CENPE with the kinetochore. Plays a role in ciliogenesis and collective cell movements. -
Tissue specificity
Widely expressed. -
Sequence similarities
Belongs to the septin family. -
Cellular localization
Cytoplasm. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle. Cleavage furrow. Midbody. Cytoplasm > cytoskeleton > cilium axoneme. Distributed throughout the cytoplasm in prometaphase cells. Associated with the spindle during metaphase. Associated with the central spindle and at the cleavage furrow in anaphase cells. Detected at the midbody in telophase. Associated with actin stress fibers. - Information by UniProt
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Database links
- Entrez Gene: 989 Human
- Entrez Gene: 235072 Mouse
- Entrez Gene: 64551 Rat
- Omim: 603151 Human
- SwissProt: Q16181 Human
- SwissProt: O55131 Mouse
- SwissProt: Q9WVC0 Rat
- Unigene: 191346 Human
see all -
Alternative names
- CDC10 antibody
- CDC10 protein homolog antibody
- CDC3 antibody
see all
Images
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Western blot - Anti-SEPT7 antibody [EPR12610] (ab175229)All lanes : Anti-SEPT7 antibody [EPR12610] (ab175229) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 51 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-SEPT7 antibody [EPR12610] (ab175229)](https://www.abcam.com/ps/products/175/ab175229/Images/ab175229-201577-anti-sept7-antibody-epr12610-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SEPT7 antibody [EPR12610] (ab175229)Immunofluorescent analysis of HeLa cells labeling SEPT7 with ab175229 at 1/50 dilution. DAPI nuclear staining (blue).
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![Anti-SEPT7 antibody [EPR12610] (ab175229)](https://www.abcam.com/ps/products/175/ab175229/Images/ab175229-3-benefits-of-recombinant-antibodies.png)
Anti-SEPT7 antibody [EPR12610] (ab175229)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab175229 has been referenced in 2 publications.
- Li R et al. Septin 7 mediates high glucose-induced podocyte apoptosis. Biochem Biophys Res Commun 506:522-528 (2018). PubMed: 30361092
- Sinclair LI & Love S Effect of APOE Genotype on Synaptic Proteins in Earlier Adult Life. J Alzheimers Dis 59:1123-1137 (2017). PubMed: 28731446