Overview

  • Product name
  • Description
    Rabbit polyclonal to Septin 11
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Xenopus laevis, Xenopus tropicalis
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 23-264 of Human Septin 11 (UniProt ID: Q9NVA2).

  • Positive control
    • 293T, A431, H1299, HeLa, HepG2 and MOLT4 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab154638 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 49 kDa.

Target

  • Function
    Filament-forming cytoskeletal GTPase. May play a role in cytokinesis (Potential). May play a role in the cytoarchitecture of neurons, including dendritic arborization and dendritic spines, and in GABAergic synaptic connectivity (By similarity). During Listeria monocytogenes infection, not required for the bacterial entry process, but restricts its efficacy.
  • Tissue specificity
    Widely expressed, except in leukocytes.
  • Involvement in disease
    Note=A chromosomal aberration involving SEPT11 may be a cause of chronic neutrophilic leukemia. Translocation t(4;11)(q21;q23) with MLL/HRX.
  • Sequence similarities
    Belongs to the septin family.
  • Cellular localization
    Cytoplasm > cytoskeleton. Cell junction > synapse. Cell projection > dendritic spine. Cell projection > axon. Partly colocalizes with stress fibers and microtubules. During bacterial infection, displays a collar shape structure next to actin at the pole of invading bacteria.
  • Information by UniProt
  • Database links
  • Alternative names
    • SEP11_HUMAN antibody
    • SEPT 11 antibody
    • Sept11 antibody
    • Septin-11 antibody
    • Septin11 antibody
    see all

Images

  • Anti-Septin 11 antibody (ab154638) at 1/1000 dilution + H1299 whole cell lysate at 30 µg

    Predicted band size: 49 kDa



    10% SDS PAGE.

     

    Secondary antibody - goat anti-rabbit HRP (ab6721)

References

ab154638 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (undifferentiated Human ES cells on MEFs)
Permeabilization
Yes - see blocking buffer
Specification
undifferentiated Human ES cells on MEFs
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Apr 14 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (undifferentiated Human ES cells on MEFs)
Gel Running Conditions
Reduced Denaturing (12% SDS gel)
Loading amount
20 µg
Specification
undifferentiated Human ES cells on MEFs
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: rt°C

Abcam user community

Verified customer

Submitted Apr 14 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEPG2)
Specification
HEPG2
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Nov 18 2015

Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HEPG2)
Gel Running Conditions
Reduced Denaturing (12.5)
Loading amount
20 µg
Specification
HEPG2
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C

Abcam user community

Verified customer

Submitted Nov 13 2015

Application
Western blot
Loading amount
100000 cells
Gel Running Conditions
Reduced Denaturing (10% gel)
Sample
Mouse Cell lysate - whole cell (mouse embryonic stem cells)
Specification
mouse embryonic stem cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C

Abcam user community

Verified customer

Submitted Jan 07 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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