Overview

  • Product name

  • Description

    Rabbit polyclonal to Septin 11
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human Septin 11 aa 1-50. The exact sequence is proprietary. NP_060713.1.
    Sequence:

    MAVAVGRPSN EELRNLSLSG HVGFDSLPDQ LVNKSTSQGF CFNILCVGET


    Database link: Q9NVA2

  • Positive control

    • HeLa, 293T, Jurkat, mouse TCMK1, and mouse NIH3T3 cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab183529 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 49 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

Images

  • Detection of Septin 11 by Western Blot of Immunoprecipitate.
    ab183529 at 0.2µg/ml labeling Septin 11 in HeLa whole cell lysate immunoprecipitated using ab183529 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.

  • All lanes : Anti-Septin 11 antibody (ab183529) at 0.2 µg

    Lane 1 : HeLa whole cell lysate
    Lane 2 : 293T whole cell lysate
    Lane 3 : Jurkat whole cell lysate
    Lane 4 : mouse TCMK1 whole cell lysate
    Lane 5 : mouse NIH3T3 whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 49 kDa


    Exposure time: 3 minutes

References

ab183529 has not yet been referenced specifically in any publications.

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