Overview

  • Product name

    Anti-Septin 11 antibody - C-terminal
    See all Septin 11 primary antibodies
  • Description

    Rabbit polyclonal to Septin 11 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Horse, Chicken, Dog, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Xenopus tropicalis
  • Immunogen

    Synthetic peptide within Human Septin 11 aa 379-429 (C terminal). The exact sequence is proprietary. (NP_060713.1 ).
    Sequence:

    KKVEDKKKELEEEVNNFQKKKAAAQLLQSQAQQSGAQQTKKDKDKKNASF T


    Database link: Q9NVA2

  • Positive control

    • HeLa, 293T, Jurkat, TCMK-1, and NIH3T3 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab183537 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 49 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

Images

  • All lanes : Anti-Septin 11 antibody - C-terminal (ab183537) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : 293T whole cell lysate
    Lane 3 : Jurkat whole cell lysate
    Lane 4 : TCMK-1 whole cell lysate
    Lane 5 : NIH3T3 whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 49 kDa


    Exposure time: 30 seconds
  • Detection of Septin 11 in immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab183537 at 6 µg/mg lysate for IP (Lane 1) and at 0.1 µg/ml for subsequent Western blot detection. Lane 2 represents Control IgG IP.

    Detection: Chemiluminescence with an exposure time of 30 seconds.

References

ab183537 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rabbit Cell (PC3 cells (prostate))
Permeabilization
Yes - TRITON
Specification
PC3 cells (prostate)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 20% · Temperature: 25°C
Fixative
PHEMO BUFFER

Dr. Keren Tazat

Verified customer

Submitted Feb 14 2019

Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Gel Running Conditions
Reduced Denaturing (4-12% gradient bis tris)
Loading amount
10 µg
Specification
HeLa cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Nov 07 2018

Application
Western blot
Sample
Human Cell lysate - whole cell (HUES7, MCF7, MET5A)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
100000 cells
Specification
HUES7, MCF7, MET5A
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C

Abcam user community

Verified customer

Submitted Aug 18 2015

Application
Western blot
Sample
Mouse Cell lysate - whole cell (embryonic stem cells)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
100000 cells
Specification
embryonic stem cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C

Abcam user community

Verified customer

Submitted Aug 18 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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