Overview

  • Product name

  • Description

    Mouse polyclonal to Septin 2
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length Human Septin 2 (NP_001008491, amino acids 1-361)

  • Positive control

    • Human spleen tissue lysate, Septin 2 transfected 293T cell lysate, HeLa cells. This antibody gave a positive result in IHC in the following FFPE tissue: Human breast fibroadenoma.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Constituent: 2.68% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab88657 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 41 kDa.
ICC/IF Use a concentration of 10 µg/ml.
IHC-P Use a concentration of 5 µg/ml.

Target

  • Relevance

    The septins are a family of cytoskeletal GTPases that play an essential role in cytokinesis in mammalian cells. Septin 2 (Nedd5) is a mammalian septin known to associate with actin based structures such as the contractile ring and stress fibers. It is involved in cytokinesis and may assemble into a multicomponent structure.
  • Cellular localization

    Cytoplasm. Cytoplasm; cytoskeleton. Cytoplasm; cytoskeleton; spindle. Chromosome; centromere; kinetochore. Cleavage furrow. Midbody. Cytoplasm; cell cortex. Cell projection; cilium membrane.
  • Database links

  • Alternative names

    • DIFF6 antibody
    • hNEDD5 antibody
    • KIAA0158 antibody
    • NEDD 5 antibody
    • NEDD5 antibody
    • Neural precursor cell expressed developmentally down regulated 5 antibody
    • Neural precursor cell expressed developmentally down regulated protein 5 antibody
    • Pnutl3 antibody
    • Protein NEDD5 antibody
    • SEPT2 antibody
    • septin 2 antibody
    see all

Images

  • Anti-Septin 2 antibody (ab88657) at 1 µg/ml + human spleen tissue lysate at 50 µg

    Predicted band size: 41 kDa
    Observed band size: 41 kDa

  • All lanes : Anti-Septin 2 antibody (ab88657) at 1 µg/ml

    Lane 1 : SEPT2 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Predicted band size: 41 kDa
    Observed band size: 38 kDa
    why is the actual band size different from the predicted?

  • ab88657 at 10µg/ml staining SEPT2 in HeLa cells by Immunofluorescence.
  • IHC image of Septin 2 staining in Human breast fibroadenoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab88657, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

This product has been referenced in:

  • Xu C  et al. Coupling function of cyclin-dependent kinase 2 and Septin2 in the promotion of hepatocellular carcinoma. Cancer Sci 110:540-549 (2019). Read more (PubMed: 30444001) »
  • Omrane M  et al. Septin 9 has Two Polybasic Domains Critical to Septin Filament Assembly and Golgi Integrity. iScience 13:138-153 (2019). Read more (PubMed: 30831549) »
See all 4 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement of ab88657. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.  

Read More
Application
Western blot
Sample
Human Tissue lysate - whole (stomach)
Loading amount
30 µg
Specification
stomach
Gel Running Conditions
Reduced Denaturing (12 %)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jul 13 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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