Key features and details
- Mouse polyclonal to Septin 2
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Septin 2 antibody
See all Septin 2 primary antibodies
DescriptionMouse polyclonal to Septin 2
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Full length Human Septin 2 (NP_001008491, amino acids 1-361)
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.4
Constituent: 2.68% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab88657 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 41 kDa.|
|ICC/IF||Use a concentration of 10 µg/ml.|
RelevanceThe septins are a family of cytoskeletal GTPases that play an essential role in cytokinesis in mammalian cells. Septin 2 (Nedd5) is a mammalian septin known to associate with actin based structures such as the contractile ring and stress fibers. It is involved in cytokinesis and may assemble into a multicomponent structure.
Cellular localizationCytoplasm. Cytoplasm; cytoskeleton. Cytoplasm; cytoskeleton; spindle. Chromosome; centromere; kinetochore. Cleavage furrow. Midbody. Cytoplasm; cell cortex. Cell projection; cilium membrane.
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Anti-Septin 2 antibody (ab88657) at 1 µg/ml + human spleen tissue lysate at 50 µg
Predicted band size: 41 kDa
Observed band size: 41 kDa
All lanes : Anti-Septin 2 antibody (ab88657) at 1 µg/ml
Lane 1 : SEPT2 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 41 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
ab88657 at 10µg/ml staining SEPT2 in HeLa cells by Immunofluorescence.
ab88657 has been referenced in 4 publications.
- Xu C et al. Coupling function of cyclin-dependent kinase 2 and Septin2 in the promotion of hepatocellular carcinoma. Cancer Sci 110:540-549 (2019). PubMed: 30444001
- Omrane M et al. Septin 9 has Two Polybasic Domains Critical to Septin Filament Assembly and Golgi Integrity. iScience 13:138-153 (2019). PubMed: 30831549
- Akil A et al. Septin 9 induces lipid droplets growth by a phosphatidylinositol-5-phosphate and microtubule-dependent mechanism hijacked by HCV. Nat Commun 7:12203 (2016). WB, ICC/IF ; Human . PubMed: 27417143
- Kocevar N et al. Proteomic analysis of gastric cancer and immunoblot validation of potential biomarkers. World J Gastroenterol 18:1216-28 (2012). WB ; Human . PubMed: 22468085