Overview

  • Product name
    Anti-Septin 2 antibody - Aminoterminal end
    See all Septin 2 primary antibodies
  • Description
    Rabbit polyclonal to Septin 2 - Aminoterminal end
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to N-terminal residues of human Septin 2

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab58657 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 41 kDa.
ELISA Use at an assay dependent dilution.

Target

  • Relevance
    The septins are a family of cytoskeletal GTPases that play an essential role in cytokinesis in mammalian cells. Septin 2 (Nedd5) is a mammalian septin known to associate with actin based structures such as the contractile ring and stress fibers. It is involved in cytokinesis and may assemble into a multicomponent structure.
  • Cellular localization
    Cytoplasm. Cytoplasm; cytoskeleton. Cytoplasm; cytoskeleton; spindle. Chromosome; centromere; kinetochore. Cleavage furrow. Midbody. Cytoplasm; cell cortex. Cell projection; cilium membrane.
  • Database links
  • Alternative names
    • DIFF6 antibody
    • hNEDD5 antibody
    • KIAA0158 antibody
    • NEDD 5 antibody
    • NEDD5 antibody
    • Neural precursor cell expressed developmentally down regulated 5 antibody
    • Neural precursor cell expressed developmentally down regulated protein 5 antibody
    • Pnutl3 antibody
    • Protein NEDD5 antibody
    • SEPT2 antibody
    • septin 2 antibody
    see all

Images

  • ab58657 staining Septin 2 in murine oocytes by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.5% Triton X-100 and then blocked using 1% BSA for 1 hour at 25°C. Samples were then incubated with primary antibody at a 1/25 dilution for 14 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Cy5®, used undiluted.

    See Abreview

References

This product has been referenced in:
  • Zhu JL  et al. Septin2 is modified by SUMOylation and required for chromosome congression in mouse oocytes. Cell Cycle 9: (2010). WB, ICC/IF ; Mouse . Read more (PubMed: 20372094) »
  • Aminin DL  et al. Immunomodulatory effects of holothurian triterpene glycosides on mammalian splenocytes determined by mass spectrometric proteome analysis. J Proteomics 72:886-906 (2009). Read more (PubMed: 19410666) »
See all 2 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement of ab88657. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.  

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (oocyte)
Loading amount
200 cells
Specification
oocyte
Gel Running Conditions
Non-reduced Denaturing (10)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Oct 13 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (oocyte)
Specification
oocyte
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% tritonX-100 in PBS
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10µg/mL · Temperature: 25°C

Abcam user community

Verified customer

Submitted Oct 13 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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