Product nameAnti-Septin 2 antibody - C-terminal
See all Septin 2 primary antibodies
DescriptionRabbit polyclonal to Septin 2 - C-terminal
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla
- HeLa, 293T, Jurkat, mouse TCMK1 and mouse NIH 3T3 whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab185998 was affinity purified using an epitope specific to Septin 2 immobilized on solid support.
Our Abpromise guarantee covers the use of ab185998 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 41 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
RelevanceThe septins are a family of cytoskeletal GTPases that play an essential role in cytokinesis in mammalian cells. Septin 2 (Nedd5) is a mammalian septin known to associate with actin based structures such as the contractile ring and stress fibers. It is involved in cytokinesis and may assemble into a multicomponent structure.
Cellular localizationCytoplasm. Cytoplasm; cytoskeleton. Cytoplasm; cytoskeleton; spindle. Chromosome; centromere; kinetochore. Cleavage furrow. Midbody. Cytoplasm; cell cortex. Cell projection; cilium membrane.
- DIFF6 antibody
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All lanes : Anti-Septin 2 antibody - C-terminal (ab185998) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK1 whole cell lysate
Lane 5 : NIH 3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 41 kDa
Exposure time: 30 seconds
Detection of Human Septin 2 by Western Blot of Immunoprecipitates of 293T
whole cell lysates (1 mg for IP; 20% of IP loaded).
Lane 1: ab185998 used for IP at 6 μg/mg lysate.
Lane 2: Control IgG.
For blotting, ab185998 was used at 1 μg/ml.
Detection: Chemiluminescence with an exposure time of 3 seconds.
ab185998 has been referenced in 1 publication.
- Li H et al. Zika Virus Protease Cleavage of Host Protein Septin-2 Mediates Mitotic Defects in Neural Progenitors. Neuron 101:1089-1098.e4 (2019). PubMed: 30713029