Recombinant
RabMAb

Recombinant Anti-Septin 2 antibody [EPR12123] (ab179436)

Overview

  • Product name

    Anti-Septin 2 antibody [EPR12123]
    See all Septin 2 primary antibodies
  • Description

    Rabbit monoclonal [EPR12123] to Septin 2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Xenopus laevis
  • Immunogen

    Synthetic peptide within Human Septin 2 aa 250-350 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q15019

  • Positive control

    • Human fetal brain, U87-MG, Jurkat and MCF-7 lysates. Human hepatocellular carcinoma and pancreas tissues. MCF-7 and Jurkat cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR12123
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab179436 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 41 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/50 - 1/100.
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Relevance

    The septins are a family of cytoskeletal GTPases that play an essential role in cytokinesis in mammalian cells. Septin 2 (Nedd5) is a mammalian septin known to associate with actin based structures such as the contractile ring and stress fibers. It is involved in cytokinesis and may assemble into a multicomponent structure.
  • Cellular localization

    Cytoplasm. Cytoplasm; cytoskeleton. Cytoplasm; cytoskeleton; spindle. Chromosome; centromere; kinetochore. Cleavage furrow. Midbody. Cytoplasm; cell cortex. Cell projection; cilium membrane.
  • Database links

    see all
  • Alternative names

    • DIFF6 antibody
    • hNEDD5 antibody
    • KIAA0158 antibody
    • NEDD 5 antibody
    • NEDD5 antibody
    • Neural precursor cell expressed developmentally down regulated 5 antibody
    • Neural precursor cell expressed developmentally down regulated protein 5 antibody
    • Pnutl3 antibody
    • Protein NEDD5 antibody
    • SEPT2 antibody
    • septin 2 antibody
    see all

Images

  • All lanes : Anti-Septin 2 antibody [EPR12123] (ab179436) at 1/1000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : U87-MG lysate
    Lane 3 : Jurkat lysate
    Lane 4 : MCF-7 lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 41 kDa

  • Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Septin 2 with ab179436 at 1/50 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Septin 2 with ab179436 at 1/50 dilution.

  • Immunofluorescent analysis of MCF-7 cells labeling Septin 2 with ab179436 at 1/50 dilution (red). DAPI nuclear staining (blue).

  • Flow cytometric analysis of permeabilized Jurkat cells labeling Septin 2 with ab179436 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).

  • Western blot analysis on immunoprecipitation pellet from (1) MCF-7 cell lysate or (2) 1XPBS (negative control) using ab179436 at a 1/10 dilution for IP, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

References

This product has been referenced in:

  • Xu C  et al. Coupling function of cyclin-dependent kinase 2 and Septin2 in the promotion of hepatocellular carcinoma. Cancer Sci 110:540-549 (2019). Read more (PubMed: 30444001) »
  • Danson CM  et al. Sorting nexin-21 is a scaffold for the endosomal recruitment of huntingtin. J Cell Sci 131:N/A (2018). Read more (PubMed: 30072438) »
See all 2 Publications for this product

Customer reviews and Q&As

Answer

Yes, we expect that ab179436 will recognize Xenopus laevis Septin 2, given that the amino acid sequence of the immunogen that generated the antibody is conserved 100% in the X. laevis protein. However, we have not tested and confirmed reactivity, so our guarantee www.abcam.com/abpromise) does not apply to this species.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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