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    septin-3-antibody-ab113983.pdf

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Cell Biology Cell Cycle Cell Division Cytokinesis
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Anti-Septin 3 antibody (ab113983)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Septin 3 antibody (ab113983)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Septin 3 antibody (ab113983)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Septin 3 antibody (ab113983)
  • Western blot - Anti-Septin 3 antibody (ab113983)

Key features and details

  • Goat polyclonal to Septin 3
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
Protein
Product image
Recombinant Human Septin 3 protein (ab202152)

View more associated products

Overview

  • Product name

    Anti-Septin 3 antibody
    See all Septin 3 primary antibodies
  • Description

    Goat polyclonal to Septin 3
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Synthetic peptide: C-

    SELVPEPRPKPA

    (with a Cysteine residue linker), corresponding to N terminal amino acids 15-26 of Human Septin 3
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Human Brain, Cerebellum Human Brain, Cortex Human Testis Human Brain lysate
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Cytokinesis

Associated products

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)
  • Recombinant Protein

    • Recombinant Human Septin 3 protein (ab202152)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab113983 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 41 kDa.
IHC-P
Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 41 kDa.
IHC-P
Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Filament-forming cytoskeletal GTPase (By similarity). May play a role in cytokinesis.
  • Tissue specificity

    Brain-specific.
  • Sequence similarities

    Belongs to the septin family.
  • Post-translational
    modifications

    Phosphorylated by PKG on serine residues. Phosphorylated by PKG on Ser-91.
  • Cellular localization

    Cytoplasm. Cytoplasm > cytoskeleton. Cell junction > synapse.
  • Target information above from: UniProt accession Q9UH03 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 618235 Cow
    • Entrez Gene: 55964 Human
    • Entrez Gene: 24050 Mouse
    • Entrez Gene: 56003 Rat
    • Omim: 608314 Human
    • SwissProt: Q08DM7 Cow
    • SwissProt: Q9UH03 Human
    • SwissProt: Q9Z1S5 Mouse
    • SwissProt: Q9WU34 Rat
    • Unigene: 120483 Human
    • Unigene: 309707 Mouse
    • Unigene: 48803 Rat
    see all
  • Alternative names

    • bK250D10.3 antibody
    • MGC133218 antibody
    • Neuronal specific septin 3 antibody
    • Neuronal-specific septin-3 antibody
    • OTTHUMP00000028742 antibody
    • SEP 3 antibody
    • SEP3 antibody
    • SEPT 3 antibody
    • sept3 antibody
    • SEPT3 protein antibody
    • SEPT3_HUMAN antibody
    • Septin3 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Septin 3 antibody (ab113983)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Septin 3 antibody (ab113983)
    ab113983, at 3.75 µg/ml, staining Septin 3 in formalin fixed paraffin embedded Human Brain (Cerebellum) by Immunohistochemistry.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Septin 3 antibody (ab113983)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Septin 3 antibody (ab113983)
    ab113983, at 3.75 µg/ml, staining Septin 3 in formalin fixed paraffin embedded Human Brain (Cortex) by Immunohistochemistry.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Septin 3 antibody (ab113983)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Septin 3 antibody (ab113983)
    ab113983, at 3.75 µg/ml, staining Septin 3 in formalin fixed paraffin embedded Human Testis by Immunohistochemistry.
  • Western blot - Anti-Septin 3 antibody (ab113983)
    Western blot - Anti-Septin 3 antibody (ab113983)
    Anti-Septin 3 antibody (ab113983) at 1 µg/ml + Human Brain lysate in RIPA buffer at 30 µg

    Predicted band size: 41 kDa



    ab113983 incubated for 1 hour. Detected using chemiluminescence.

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab113983? Please let us know so that we can cite the reference in this datasheet.

ab113983 has not yet been referenced specifically in any publications.

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