Anti-Septin 3 antibody (ab113983)
Key features and details
- Goat polyclonal to Septin 3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Septin 3 antibody
See all Septin 3 primary antibodies -
Description
Goat polyclonal to Septin 3 -
Host species
Goat -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow
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Immunogen
Synthetic peptide: C-
SELVPEPRPKPA
(with a Cysteine residue linker), corresponding to N terminal amino acids 15-26 of Human Septin 3 -
Positive control
- Human Brain, Cerebellum Human Brain, Cortex Human Testis Human Brain lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab113983 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 41 kDa.
|
|
| IHC-P |
Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 41 kDa. |
|
IHC-P
Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Filament-forming cytoskeletal GTPase (By similarity). May play a role in cytokinesis. -
Tissue specificity
Brain-specific. -
Sequence similarities
Belongs to the septin family. -
Post-translational
modificationsPhosphorylated by PKG on serine residues. Phosphorylated by PKG on Ser-91. -
Cellular localization
Cytoplasm. Cytoplasm > cytoskeleton. Cell junction > synapse. - Information by UniProt
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Database links
- Entrez Gene: 618235 Cow
- Entrez Gene: 55964 Human
- Entrez Gene: 24050 Mouse
- Entrez Gene: 56003 Rat
- Omim: 608314 Human
- SwissProt: Q08DM7 Cow
- SwissProt: Q9UH03 Human
- SwissProt: Q9Z1S5 Mouse
see all -
Alternative names
- bK250D10.3 antibody
- MGC133218 antibody
- Neuronal specific septin 3 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Septin 3 antibody (ab113983)ab113983, at 3.75 µg/ml, staining Septin 3 in formalin fixed paraffin embedded Human Brain (Cerebellum) by Immunohistochemistry.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Septin 3 antibody (ab113983)ab113983, at 3.75 µg/ml, staining Septin 3 in formalin fixed paraffin embedded Human Brain (Cortex) by Immunohistochemistry. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Septin 3 antibody (ab113983)ab113983, at 3.75 µg/ml, staining Septin 3 in formalin fixed paraffin embedded Human Testis by Immunohistochemistry. -
Western blot - Anti-Septin 3 antibody (ab113983)Anti-Septin 3 antibody (ab113983) at 1 µg/ml + Human Brain lysate in RIPA buffer at 30 µg
Predicted band size: 41 kDa
ab113983 incubated for 1 hour. Detected using chemiluminescence.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab113983 has not yet been referenced specifically in any publications.