- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-Septin 3 antibody
See all Septin 3 primary antibodies -
DescriptionGoat polyclonal to Septin 3
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Host speciesGoat
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Tested applicationsSuitable for: WB, IHC-Pmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
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Immunogen
Synthetic peptide: C-
SELVPEPRPKPA
(with a Cysteine residue linker), corresponding to N terminal amino acids 15-26 of Human Septin 3 -
Positive control
- Human Brain, Cerebellum Human Brain, Cortex Human Testis Human Brain lysate
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20ºC.
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Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab113983 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 41 kDa. | |
| IHC-P | Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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FunctionFilament-forming cytoskeletal GTPase (By similarity). May play a role in cytokinesis.
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Tissue specificityBrain-specific.
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Sequence similaritiesBelongs to the septin family.
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Post-translational
modificationsPhosphorylated by PKG on serine residues. Phosphorylated by PKG on Ser-91. -
Cellular localizationCytoplasm. Cytoplasm > cytoskeleton. Cell junction > synapse.
- Information by UniProt
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Database links
- Entrez Gene: 618235 Cow
- Entrez Gene: 55964 Human
- Entrez Gene: 24050 Mouse
- Entrez Gene: 56003 Rat
- Omim: 608314 Human
- SwissProt: Q08DM7 Cow
- SwissProt: Q9UH03 Human
- SwissProt: Q9Z1S5 Mouse
see all -
Alternative names
- bK250D10.3 antibody
- MGC133218 antibody
- Neuronal specific septin 3 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Septin 3 antibody (ab113983)ab113983, at 3.75 µg/ml, staining Septin 3 in formalin fixed paraffin embedded Human Brain (Cerebellum) by Immunohistochemistry. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Septin 3 antibody (ab113983)ab113983, at 3.75 µg/ml, staining Septin 3 in formalin fixed paraffin embedded Human Brain (Cortex) by Immunohistochemistry. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Septin 3 antibody (ab113983)ab113983, at 3.75 µg/ml, staining Septin 3 in formalin fixed paraffin embedded Human Testis by Immunohistochemistry. -
Western blot - Anti-Septin 3 antibody (ab113983)Anti-Septin 3 antibody (ab113983) at 1 µg/ml + Human Brain lysate in RIPA buffer at 30 µg
Predicted band size: 41 kDa
ab113983 incubated for 1 hour. Detected using chemiluminescence.
References
ab113983 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"