Key features and details
- Rabbit polyclonal to Septin 3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Septin 3 antibody
See all Septin 3 primary antibodies
DescriptionRabbit polyclonal to Septin 3
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
- WB: HEK-293T overexpressing Septin 3 cell lysate. IHC-P: Human lateral ventricle tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab224332 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 41 kDa.|
|IHC-P||1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionFilament-forming cytoskeletal GTPase (By similarity). May play a role in cytokinesis.
Sequence similaritiesBelongs to the septin family.
modificationsPhosphorylated by PKG on serine residues. Phosphorylated by PKG on Ser-91.
Cellular localizationCytoplasm. Cytoplasm > cytoskeleton. Cell junction > synapse.
- Information by UniProt
- bK250D10.3 antibody
- MGC133218 antibody
- Neuronal specific septin 3 antibody
Paraffin-embedded human lateral ventricle tissue stained for Septin 3 using ab224332 at 1/500 dilution in immunohistochemical analysis.
All lanes : Anti-Septin 3 antibody (ab224332) at 1/100 dilution
Lane 1 : Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : HEK-293T overexpressing Septin 3 (co-expressed with a C terminal myc-DDK tag), cell lysate
Developed using the ECL technique.
Predicted band size: 41 kDa
ab224332 has not yet been referenced specifically in any publications.