Overview

  • Product name
    Anti-Septin 8 antibody
    See all Septin 8 primary antibodies
  • Description
    Rabbit polyclonal to Septin 8
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow, Xenopus tropicalis
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 174-458 of Human Septin 8 (UniProt ID: Q92599).

  • Positive control
    • A549, HeLa, HepG2 and HCT116 whole cell lysates; HeLa cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154112 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 56 kDa.
ICC/IF 1/100 - 1/1000.

Target

Images

  • Western blot - Anti-Septin 8 antibody (ab154112)
    Western blot - Anti-Septin 8 antibody (ab154112)
    Anti-Septin 8 antibody (ab154112) at 1/2000 dilution + HCT116 whole cell lysate at 30 µg

    Predicted band size: 56 kDa



    12% SDS PAGE
  • Immunocytochemistry/ Immunofluorescence - Anti-Septin 8 antibody (ab154112)
    Immunocytochemistry/ Immunofluorescence - Anti-Septin 8 antibody (ab154112)
    Immunofluorescent analysis of methanol-fixed HeLa cells labeling Septin 8 with ab154112 at 1/200 dilution (green).
    Alpha tubulin filaments were labeled with an Alpha tubulin antibody (red).

References

ab154112 has not yet been referenced specifically in any publications.

Publishing research using ab154112? Please let us know so that we can cite the reference in this datasheet.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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