Anti-Septin 8 antibody (ab154112)
Key features and details
- Rabbit polyclonal to Septin 8
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Septin 8 antibody
See all Septin 8 primary antibodies -
Description
Rabbit polyclonal to Septin 8 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Cow, Xenopus tropicalis
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 174-458 of Human Septin 8 (UniProt ID: Q92599).
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Positive control
- A549, HeLa, HepG2 and HCT116 whole cell lysates; HeLa cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154112 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 56 kDa.
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| ICC/IF |
1/100 - 1/1000.
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| Notes |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 56 kDa. |
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ICC/IF
1/100 - 1/1000. |
Target
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Relevance
Septin 8 (SEPT8) is a member of the highly conserved septin family. Septins are 40- to 60-kD GTPases that assemble as filamentous scaffolds. They are involved in the organization of submembranous structures, in neuronal polarity, and in vesicle trafficking. There are 3 isoforms produced by alternative splicing. -
Cellular localization
Cytoplasmic; cytoskeleton. -
Database links
- Entrez Gene: 540614 Cow
- Entrez Gene: 23176 Human
- Entrez Gene: 20362 Mouse
- Entrez Gene: 303135 Rat
- Entrez Gene: 100126684 Xenopus tropicalis
- Omim: 608418 Human
- SwissProt: Q0VCP4 Cow
- SwissProt: Q92599 Human
see all -
Alternative names
- KIAA0202 antibody
- OTTHUMP00000211755 antibody
- SEP 2 antibody
see all
Images
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Western blot - Anti-Septin 8 antibody (ab154112)Anti-Septin 8 antibody (ab154112) at 1/2000 dilution + HCT116 whole cell lysate at 30 µg
Predicted band size: 56 kDa
12% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-Septin 8 antibody (ab154112)Immunofluorescent analysis of methanol-fixed HeLa cells labeling Septin 8 with ab154112 at 1/200 dilution (green).
Alpha tubulin filaments were labeled with an Alpha tubulin antibody (red).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab154112 has not yet been referenced specifically in any publications.