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    septin-8-antibody-ab154112.pdf

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Cell Biology Cell Cycle Cell Division Cytokinesis
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Anti-Septin 8 antibody (ab154112)

  • Datasheet
  • SDS
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Western blot - Anti-Septin 8 antibody (ab154112)
  • Immunocytochemistry/ Immunofluorescence - Anti-Septin 8 antibody (ab154112)

Key features and details

  • Rabbit polyclonal to Septin 8
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-Septin 8 antibody
    See all Septin 8 primary antibodies
  • Description

    Rabbit polyclonal to Septin 8
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow, Xenopus tropicalis
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 174-458 of Human Septin 8 (UniProt ID: Q92599).

  • Positive control

    • A549, HeLa, HepG2 and HCT116 whole cell lysates; HeLa cells
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Cytokinesis

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Related Products

    • HeLa whole cell lysate (ab29545)
    • A549 whole cell lysate (ab7910)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab154112 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 56 kDa.
ICC/IF
1/100 - 1/1000.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 56 kDa.
ICC/IF
1/100 - 1/1000.

Target

  • Relevance

    Septin 8 (SEPT8) is a member of the highly conserved septin family. Septins are 40- to 60-kD GTPases that assemble as filamentous scaffolds. They are involved in the organization of submembranous structures, in neuronal polarity, and in vesicle trafficking. There are 3 isoforms produced by alternative splicing.
  • Cellular localization

    Cytoplasmic; cytoskeleton.
  • Database links

    • Entrez Gene: 540614 Cow
    • Entrez Gene: 23176 Human
    • Entrez Gene: 20362 Mouse
    • Entrez Gene: 303135 Rat
    • Entrez Gene: 100126684 Xenopus tropicalis
    • Omim: 608418 Human
    • SwissProt: Q0VCP4 Cow
    • SwissProt: Q92599 Human
    • SwissProt: Q8CHH9 Mouse
    • SwissProt: B0BNF1 Rat
    • SwissProt: B1H120 Xenopus tropicalis
    • Unigene: 522057 Human
    • Unigene: 274399 Mouse
    • Unigene: 23507 Rat
    see all
  • Alternative names

    • KIAA0202 antibody
    • OTTHUMP00000211755 antibody
    • SEP 2 antibody
    • SEP2 antibody
    • SEPT 8 antibody
    • SEPT8 antibody
    • Septin8 antibody
    see all

Images

  • Western blot - Anti-Septin 8 antibody (ab154112)
    Western blot - Anti-Septin 8 antibody (ab154112)
    Anti-Septin 8 antibody (ab154112) at 1/2000 dilution + HCT116 whole cell lysate at 30 µg

    Predicted band size: 56 kDa



    12% SDS PAGE
  • Immunocytochemistry/ Immunofluorescence - Anti-Septin 8 antibody (ab154112)
    Immunocytochemistry/ Immunofluorescence - Anti-Septin 8 antibody (ab154112)
    Immunofluorescent analysis of methanol-fixed HeLa cells labeling Septin 8 with ab154112 at 1/200 dilution (green).
    Alpha tubulin filaments were labeled with an Alpha tubulin antibody (red).

Protocols

  • Western blot protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab154112? Please let us know so that we can cite the reference in this datasheet.

ab154112 has not yet been referenced specifically in any publications.

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