Recombinant
RabMAb

Recombinant Anti-Septin 8 antibody [EPR16099] (ab191404)

Overview

  • Product name

    Anti-Septin 8 antibody [EPR16099]
    See all Septin 8 primary antibodies
  • Description

    Rabbit monoclonal [EPR16099] to Septin 8
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Septin 8 aa 50-150. The exact sequence is proprietary.
    Database link: Q92599

  • Positive control

    • K562, HeLa, HCT-116, U-87 MG, C6, PC-12 and NIH/3T3 cell lysates; Human stomach and mouse brain tissues; K562 and C6 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR16099
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab191404 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
IHC-P 1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/200.
Flow Cyt 1/150.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IP 1/50.

Target

Images

  • All lanes : Anti-Septin 8 antibody [EPR16099] (ab191404) at 1/10000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Predicted band size: 56 kDa
    Observed band size: 56 kDa



    Blocking/Dilution buffer: 5% NFDM /TBST.

  • All lanes : Anti-Septin 8 antibody [EPR16099] (ab191404) at 1/1000 dilution

    Lane 1 : HCT-116 cell lysate
    Lane 2 : U-87 MG cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Predicted band size: 56 kDa
    Observed band size: 56 kDa



    Blocking/Dilution buffer: 5% NFDM /TBST.

  • All lanes : Anti-Septin 8 antibody [EPR16099] (ab191404) at 1/1000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : PC-12 cell lysate
    Lane 3 : NIH/3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Predicted band size: 56 kDa
    Observed band size: 56 kDa



    Blocking/Dilution buffer: 5% NFDM /TBST.

  • Immunohistochemical analysis of paraffin-embedded, Human stomach tissue labeling Septin 8 with ab191404 at 1/200 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. In the negative control PBS was used instead of primary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue labeling Septin 8 with ab191404 at 1/200 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. In the negative control PBS was used instead of primary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized C6 cells labeling Septin 8 with ab191404 at 1/200 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor®488) secondary antibody (ab150077) at 1/200 dilution. Nuclear counter stain DAPI (blue).

    The two negative controls used anti-Septin 8 primary antibody at 1/200 dilution followed by Goat anti-mouse IgG (Alexa Fluor®594) secondary antibody at 1/400 dilution.

  • Flow cytometric analysis of 2% paraformaldehyde-fixed K562 cells labeling Septin 8 with ab191404 at 1/150 dilution (pink), compared to a Rabbit monoclonal IgG isotype control (green). Goat anti-rabbit IgG (FITC) was used as secondary antibody at 1/150 dilution.

  • Western blot analysis of Septin 8 in K562 cell lysate immunoprecipitated using ab191404 at 1/50 dilution (Lane 1). Lane 2: PBS instead of K562 lysates.

    Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

References

ab191404 has not yet been referenced specifically in any publications.

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