Recombinant
RabMAb

Recombinant Anti-SERCA1 ATPase antibody [EPR7321] (ab133275)

Overview

  • Product name

    Anti-SERCA1 ATPase antibody [EPR7321]
    See all SERCA1 ATPase primary antibodies
  • Description

    Rabbit monoclonal [EPR7321] to SERCA1 ATPase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human SERCA1 ATPase aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: O14983

  • Positive control

    • fetal muscle lysate human skeletal muscle tissue
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133275 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 110 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Key regulator of striated muscle performance by acting as the major Ca(2+) ATPase responsible for the reuptake of cytosolic Ca(2+) into the sarcoplasmic reticulum. Catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction.
  • Tissue specificity

    Skeletal muscle, fast twitch muscle (type II) fibers.
  • Involvement in disease

    Brody myopathy
  • Sequence similarities

    Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily.
  • Developmental stage

    Isoform SERCA1A accounts for more than 99% of SERCA1 isoforms expressed in adult skeletal muscle, while isoform SERCA1B predominates in neo-natal skeletal muscle.
  • Cellular localization

    Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • fast twitch skeletal muscle isoform antibody
    • AT2A1_HUMAN antibody
    • ATP2A antibody
    • ATP2A1 antibody
    • ATPase Ca++ transporting cardiac muscle fast twitch 1 antibody
    • ATPase Ca++ transporting fast twitch 1 antibody
    • ATPase, Ca(2+)-transporting fast twitch 1 antibody
    • Calcium pump 1 antibody
    • Calcium transporting ATPase sarcoplasmic reticulum type fast twitch skeletal muscle isoform antibody
    • Calcium-transporting ATPase sarcoplasmic reticulum type antibody
    • EC 3.6.3.8 antibody
    • Endoplasmic reticulum class 1/2 Ca(2+) ATPase antibody
    • Fast skeletal muscle SR calcium ATPase antibody
    • OTTHUMP00000162561 antibody
    • OTTHUMP00000162562 antibody
    • Sarcoendoplasmic reticulum calcium ATPase antibody
    • Sarcoplasmic reticulum Ca(2+)-ATPase 1 antibody
    • Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 antibody
    • SERCA 1 antibody
    • SERCA1 antibody
    • SERCA1 truncated isoform, included antibody
    • SR Ca(2+) ATPase 1 antibody
    • SR Ca(2+)-ATPase 1 antibody
    see all

Images

  • Anti-SERCA1 ATPase antibody [EPR7321] (ab133275) at 1/10000 dilution + fetal muscle lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 110 kDa
    Observed band size: 100 kDa
    why is the actual band size different from the predicted?



    Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 180 seconds.

  • Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labelling SERCA1 ATPase using ab133275, at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

References

ab133275 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab133275.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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