Name: Anti-SERCA1 ATPase antibody [EPR7322]
Brand: RabMAb
SKU: ab129104
Rating: 4 (1 reviews)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

Loading size & price…

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-SERCA1 ATPase antibody [EPR7322] (ab129104)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR7322] to SERCA1 ATPase
Suitable for: WB
Reacts with: Mouse, Rat, Human

Carrier Free

Product name

Anti-SERCA1 ATPase antibody [EPR7322]
See all SERCA1 ATPase primary antibodies
Description

Rabbit monoclonal [EPR7322] to SERCA1 ATPase
Host species

Rabbit
Specificity

ab129104 can not recognize SERCA1A (ATP2A1A). ab129104 recognizes SERCA1B (ATP2A1B) only.
Tested applications

Suitable for: WBmore details
Unsuitable for: IHC-P or IP
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide within Human SERCA1 ATPase aa 950-1050. The exact sequence is proprietary.
Positive control
- Human fetal muscle, Rat muscle and Mouse muscle lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (K_D)

K_D = 4.50 x 10 ^-11 M
Learn more about K_D
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR7322
Isotype

IgG
Research areas

Alternative Versions
- Anti-SERCA1 ATPase antibody [EPR7322] - BSA and Azide free (ab248302)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant Human SERCA1 ATPase protein (ab152208)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab129104 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB	(1)	1/1000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 110 kDa).

Notes
WB 1/1000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 110 kDa).

Application notes

Is unsuitable for IHC-P or IP.

Function

Key regulator of striated muscle performance by acting as the major Ca(2+) ATPase responsible for the reuptake of cytosolic Ca(2+) into the sarcoplasmic reticulum. Catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction.
Tissue specificity

Skeletal muscle, fast twitch muscle (type II) fibers.
Involvement in disease

Brody myopathy
Sequence similarities

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily.
Developmental stage

Isoform SERCA1A accounts for more than 99% of SERCA1 isoforms expressed in adult skeletal muscle, while isoform SERCA1B predominates in neo-natal skeletal muscle.
Cellular localization

Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane.
Target information above from: UniProt accession O14983 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 487 Human
- Entrez Gene: 11937 Mouse
- Entrez Gene: 116601 Rat
- Omim: 108730 Human
- SwissProt: O14983 Human
- SwissProt: Q8R429 Mouse
- SwissProt: Q64578 Rat
- Unigene: 657344 Human
- Unigene: 35134 Mouse
- Unigene: 217139 Rat
see all
Alternative names
- fast twitch skeletal muscle isoform antibody
- AT2A1_HUMAN antibody
- ATP2A antibody
- ATP2A1 antibody
- ATPase Ca++ transporting cardiac muscle fast twitch 1 antibody
- ATPase Ca++ transporting fast twitch 1 antibody
- ATPase, Ca(2+)-transporting fast twitch 1 antibody
- Calcium pump 1 antibody
- Calcium transporting ATPase sarcoplasmic reticulum type fast twitch skeletal muscle isoform antibody
- Calcium-transporting ATPase sarcoplasmic reticulum type antibody
- EC 3.6.3.8 antibody
- Endoplasmic reticulum class 1/2 Ca(2+) ATPase antibody
- Fast skeletal muscle SR calcium ATPase antibody
- OTTHUMP00000162561 antibody
- OTTHUMP00000162562 antibody
- Sarcoendoplasmic reticulum calcium ATPase antibody
- Sarcoplasmic reticulum Ca(2+)-ATPase 1 antibody
- Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 antibody
- SERCA 1 antibody
- SERCA1 antibody
- SERCA1 truncated isoform, included antibody
- SR Ca(2+) ATPase 1 antibody
- SR Ca(2+)-ATPase 1 antibody
see all

Western blot - Anti-SERCA1 ATPase antibody [EPR7322] (ab129104)

All lanes : Anti-SERCA1 ATPase antibody [EPR7322] (ab129104) at 1/1000 dilution

Lane 1 : Human fetal muscle lysate
Lane 2 : Rat muscle lysate
Lane 3 : Mouse muscle lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 110 kDa
OI-RD Scanning - Anti-SERCA1 ATPase antibody [EPR7322] (ab129104)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
Anti-SERCA1 ATPase antibody [EPR7322] (ab129104)

Western blot protocols

Click here to view the general protocols

Datasheet download

Download

Publishing research using ab129104? Please let us know so that we can cite the reference in this datasheet.

ab129104 has been referenced in 1 publication.

Hook SC et al. TBC1D1 interacting proteins, VPS13A and VPS13C, regulate GLUT4 homeostasis in C2C12 myotubes. Sci Rep 10:17953 (2020). PubMed: 33087848

Customer reviews and Q&As

Show All Reviews Q&A

Submit a review Submit a question

Application

Western blot

Sample

Rabbit Tissue lysate - other (muscle and heart)

Gel Running Conditions

Reduced Denaturing (4-12%)

Loading amount

10 µg

Specification

muscle and heart

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

MRS. Minh Hien Le

Verified customer

Submitted Feb 10 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Switch on our new BETA site

Now available

In the coming months

Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Specificity

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Dissociation constant (KD)

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Isotype control

Recombinant Protein

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Involvement in disease

Sequence similarities

Developmental stage

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

Datasheet download

References (1)

Customer reviews and Q&As

Western blot abreview for Anti-SERCA1 ATPase antibody [EPR7322]

Dissociation constant (K_D)