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  1. Link

    serca1-atpase-antibody-epr7322-ab129104.pdf

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RecombinantRabMAb

Recombinant Anti-SERCA1 ATPase antibody [EPR7322] (ab129104)

  • Datasheet
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Western blot - Anti-SERCA1 ATPase antibody [EPR7322] (ab129104)
  • OI-RD Scanning - Anti-SERCA1 ATPase antibody [EPR7322] (ab129104)
  • Anti-SERCA1 ATPase antibody [EPR7322] (ab129104)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7322] to SERCA1 ATPase
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Recombinant Human SERCA1 ATPase protein (ab152208)
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Overview

  • Product name

    Anti-SERCA1 ATPase antibody [EPR7322]
    See all SERCA1 ATPase primary antibodies
  • Description

    Rabbit monoclonal [EPR7322] to SERCA1 ATPase
  • Host species

    Rabbit
  • Specificity

    ab129104 can not recognize SERCA1A (ATP2A1A). ab129104 recognizes SERCA1B (ATP2A1B) only.
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human SERCA1 ATPase aa 950-1050. The exact sequence is proprietary.

  • Positive control

    • Human fetal muscle, Rat muscle and Mouse muscle lysates.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 4.50 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR7322
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • ER
    • Signal Transduction
    • Signaling Pathway
    • Calcium Signaling
    • Calcium Channels
    • Signal Transduction
    • Metabolism
    • Plasma Membrane
    • ATPases
    • Cardiovascular
    • Atherosclerosis
    • Hypertension
    • Sodium/ion transport
    • Metabolism
    • Types of disease
    • Heart disease

Associated products

  • Alternative Versions

    • Anti-SERCA1 ATPase antibody [EPR7322] - BSA and Azide free (ab248302)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human SERCA1 ATPase protein (ab152208)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab129104 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/1000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 110 kDa).
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 110 kDa).
Application notes
Is unsuitable for IHC-P or IP.

Target

  • Function

    Key regulator of striated muscle performance by acting as the major Ca(2+) ATPase responsible for the reuptake of cytosolic Ca(2+) into the sarcoplasmic reticulum. Catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction.
  • Tissue specificity

    Skeletal muscle, fast twitch muscle (type II) fibers.
  • Involvement in disease

    Brody myopathy
  • Sequence similarities

    Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily.
  • Developmental stage

    Isoform SERCA1A accounts for more than 99% of SERCA1 isoforms expressed in adult skeletal muscle, while isoform SERCA1B predominates in neo-natal skeletal muscle.
  • Cellular localization

    Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane.
  • Target information above from: UniProt accession O14983 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 487 Human
    • Entrez Gene: 11937 Mouse
    • Entrez Gene: 116601 Rat
    • Omim: 108730 Human
    • SwissProt: O14983 Human
    • SwissProt: Q8R429 Mouse
    • SwissProt: Q64578 Rat
    • Unigene: 657344 Human
    • Unigene: 35134 Mouse
    • Unigene: 217139 Rat
    see all
  • Alternative names

    • fast twitch skeletal muscle isoform antibody
    • AT2A1_HUMAN antibody
    • ATP2A antibody
    • ATP2A1 antibody
    • ATPase Ca++ transporting cardiac muscle fast twitch 1 antibody
    • ATPase Ca++ transporting fast twitch 1 antibody
    • ATPase, Ca(2+)-transporting fast twitch 1 antibody
    • Calcium pump 1 antibody
    • Calcium transporting ATPase sarcoplasmic reticulum type fast twitch skeletal muscle isoform antibody
    • Calcium-transporting ATPase sarcoplasmic reticulum type antibody
    • EC 3.6.3.8 antibody
    • Endoplasmic reticulum class 1/2 Ca(2+) ATPase antibody
    • Fast skeletal muscle SR calcium ATPase antibody
    • OTTHUMP00000162561 antibody
    • OTTHUMP00000162562 antibody
    • Sarcoendoplasmic reticulum calcium ATPase antibody
    • Sarcoplasmic reticulum Ca(2+)-ATPase 1 antibody
    • Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 antibody
    • SERCA 1 antibody
    • SERCA1 antibody
    • SERCA1 truncated isoform, included antibody
    • SR Ca(2+) ATPase 1 antibody
    • SR Ca(2+)-ATPase 1 antibody
    see all

Images

  • Western blot - Anti-SERCA1 ATPase antibody [EPR7322] (ab129104)
    Western blot - Anti-SERCA1 ATPase antibody [EPR7322] (ab129104)
    All lanes : Anti-SERCA1 ATPase antibody [EPR7322] (ab129104) at 1/1000 dilution

    Lane 1 : Human fetal muscle lysate
    Lane 2 : Rat muscle lysate
    Lane 3 : Mouse muscle lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 110 kDa

  • OI-RD Scanning - Anti-SERCA1 ATPase antibody [EPR7322] (ab129104)
    OI-RD Scanning - Anti-SERCA1 ATPase antibody [EPR7322] (ab129104)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-SERCA1 ATPase antibody [EPR7322] (ab129104)
    Anti-SERCA1 ATPase antibody [EPR7322] (ab129104)

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (1)

Publishing research using ab129104? Please let us know so that we can cite the reference in this datasheet.

ab129104 has been referenced in 1 publication.

  • Hook SC  et al. TBC1D1 interacting proteins, VPS13A and VPS13C, regulate GLUT4 homeostasis in C2C12 myotubes. Sci Rep 10:17953 (2020). PubMed: 33087848

Customer reviews and Q&As

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Western blot abreview for Anti-SERCA1 ATPase antibody [EPR7322]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rabbit Tissue lysate - other (muscle and heart)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
10 µg
Specification
muscle and heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

MRS. Minh Hien Le

Verified customer

Submitted Feb 10 2016

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