Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7322] to SERCA1 ATPase
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Product nameAnti-SERCA1 ATPase antibody [EPR7322]
See all SERCA1 ATPase primary antibodies
DescriptionRabbit monoclonal [EPR7322] to SERCA1 ATPase
Specificityab129104 can not recognize SERCA1A (ATP2A1A). ab129104 recognizes SERCA1B (ATP2A1B) only.
Tested applicationsSuitable for: WBmore details
Unsuitable for: IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human SERCA1 ATPase aa 950-1050. The exact sequence is proprietary.
- Human fetal muscle, Rat muscle and Mouse muscle lysates.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 4.50 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab129104 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 110 kDa).|
FunctionKey regulator of striated muscle performance by acting as the major Ca(2+) ATPase responsible for the reuptake of cytosolic Ca(2+) into the sarcoplasmic reticulum. Catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction.
Tissue specificitySkeletal muscle, fast twitch muscle (type II) fibers.
Involvement in diseaseBrody myopathy
Sequence similaritiesBelongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily.
Developmental stageIsoform SERCA1A accounts for more than 99% of SERCA1 isoforms expressed in adult skeletal muscle, while isoform SERCA1B predominates in neo-natal skeletal muscle.
Cellular localizationEndoplasmic reticulum membrane. Sarcoplasmic reticulum membrane.
- Information by UniProt
- fast twitch skeletal muscle isoform antibody
- AT2A1_HUMAN antibody
- ATP2A antibody
All lanes : Anti-SERCA1 ATPase antibody [EPR7322] (ab129104) at 1/1000 dilution
Lane 1 : Human fetal muscle lysate
Lane 2 : Rat muscle lysate
Lane 3 : Mouse muscle lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 110 kDa
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab129104 has not yet been referenced specifically in any publications.