Recombinant Anti-SERCA1 ATPase antibody [EPR7322] (ab129104)


  • Product name

    Anti-SERCA1 ATPase antibody [EPR7322]
    See all SERCA1 ATPase primary antibodies
  • Description

    Rabbit monoclonal [EPR7322] to SERCA1 ATPase
  • Host species

  • Specificity

    ab129104 can not recognize SERCA1A (ATP2A1A). ab129104 recognizes SERCA1B (ATP2A1B) only.
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human SERCA1 ATPase aa 950-1050. The exact sequence is proprietary.

  • Positive control

    • Human fetal muscle, Rat muscle and Mouse muscle lysates.
  • General notes



    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab129104 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 110 kDa).
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Key regulator of striated muscle performance by acting as the major Ca(2+) ATPase responsible for the reuptake of cytosolic Ca(2+) into the sarcoplasmic reticulum. Catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction.
    • Tissue specificity

      Skeletal muscle, fast twitch muscle (type II) fibers.
    • Involvement in disease

      Brody myopathy
    • Sequence similarities

      Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily.
    • Developmental stage

      Isoform SERCA1A accounts for more than 99% of SERCA1 isoforms expressed in adult skeletal muscle, while isoform SERCA1B predominates in neo-natal skeletal muscle.
    • Cellular localization

      Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • fast twitch skeletal muscle isoform antibody
      • AT2A1_HUMAN antibody
      • ATP2A antibody
      • ATP2A1 antibody
      • ATPase Ca++ transporting cardiac muscle fast twitch 1 antibody
      • ATPase Ca++ transporting fast twitch 1 antibody
      • ATPase, Ca(2+)-transporting fast twitch 1 antibody
      • Calcium pump 1 antibody
      • Calcium transporting ATPase sarcoplasmic reticulum type fast twitch skeletal muscle isoform antibody
      • Calcium-transporting ATPase sarcoplasmic reticulum type antibody
      • EC antibody
      • Endoplasmic reticulum class 1/2 Ca(2+) ATPase antibody
      • Fast skeletal muscle SR calcium ATPase antibody
      • OTTHUMP00000162561 antibody
      • OTTHUMP00000162562 antibody
      • Sarcoendoplasmic reticulum calcium ATPase antibody
      • Sarcoplasmic reticulum Ca(2+)-ATPase 1 antibody
      • Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 antibody
      • SERCA 1 antibody
      • SERCA1 antibody
      • SERCA1 truncated isoform, included antibody
      • SR Ca(2+) ATPase 1 antibody
      • SR Ca(2+)-ATPase 1 antibody
      see all


    • All lanes : Anti-SERCA1 ATPase antibody [EPR7322] (ab129104) at 1/1000 dilution

      Lane 1 : Human fetal muscle lysate
      Lane 2 : Rat muscle lysate
      Lane 3 : Mouse muscle lysate

      Lysates/proteins at 10 µg per lane.

      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 110 kDa

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD


    ab129104 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Western blot
    Rabbit Tissue lysate - other (muscle and heart)
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Loading amount
    10 µg
    muscle and heart
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Mrs. Minh Hien Le

    Verified customer

    Submitted Feb 10 2016

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