Recombinant
RabMAb

Recombinant Anti-SERCA1 ATPase antibody [EPR7322] (ab129104)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7322] to SERCA1 ATPase
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SERCA1 ATPase antibody [EPR7322]
    See all SERCA1 ATPase primary antibodies

  • Description

    Rabbit monoclonal [EPR7322] to SERCA1 ATPase

  • Host species

    Rabbit

  • Specificity

    ab129104 can not recognize SERCA1A (ATP2A1A). ab129104 recognizes SERCA1B (ATP2A1B) only.

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human SERCA1 ATPase aa 950-1050. The exact sequence is proprietary.

  • Positive control

    • Human fetal muscle, Rat muscle and Mouse muscle lysates.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab129104 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 110 kDa).
  • Application notes
    Is unsuitable for IHC-P or IP.

    • Target

    • Function

      Key regulator of striated muscle performance by acting as the major Ca(2+) ATPase responsible for the reuptake of cytosolic Ca(2+) into the sarcoplasmic reticulum. Catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction.

    • Tissue specificity

      Skeletal muscle, fast twitch muscle (type II) fibers.

    • Involvement in disease

      Brody myopathy

    • Sequence similarities

      Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily.

    • Developmental stage

      Isoform SERCA1A accounts for more than 99% of SERCA1 isoforms expressed in adult skeletal muscle, while isoform SERCA1B predominates in neo-natal skeletal muscle.

    • Cellular localization

      Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • fast twitch skeletal muscle isoform antibody
      • AT2A1_HUMAN antibody
      • ATP2A antibody
      • ATP2A1 antibody
      • ATPase Ca++ transporting cardiac muscle fast twitch 1 antibody
      • ATPase Ca++ transporting fast twitch 1 antibody
      • ATPase, Ca(2+)-transporting fast twitch 1 antibody
      • Calcium pump 1 antibody
      • Calcium transporting ATPase sarcoplasmic reticulum type fast twitch skeletal muscle isoform antibody
      • Calcium-transporting ATPase sarcoplasmic reticulum type antibody
      • EC 3.6.3.8 antibody
      • Endoplasmic reticulum class 1/2 Ca(2+) ATPase antibody
      • Fast skeletal muscle SR calcium ATPase antibody
      • OTTHUMP00000162561 antibody
      • OTTHUMP00000162562 antibody
      • Sarcoendoplasmic reticulum calcium ATPase antibody
      • Sarcoplasmic reticulum Ca(2+)-ATPase 1 antibody
      • Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 antibody
      • SERCA 1 antibody
      • SERCA1 antibody
      • SERCA1 truncated isoform, included antibody
      • SR Ca(2+) ATPase 1 antibody
      • SR Ca(2+)-ATPase 1 antibody
      see all

    Images

    • Western blot - Anti-SERCA1 ATPase antibody [EPR7322] (ab129104)
      Western blot - Anti-SERCA1 ATPase antibody [EPR7322] (ab129104)
      All lanes : Anti-SERCA1 ATPase antibody [EPR7322] (ab129104) at 1/1000 dilution

      Lane 1 : Human fetal muscle lysate
      Lane 2 : Rat muscle lysate
      Lane 3 : Mouse muscle lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 110 kDa

    • Other - Anti-SERCA1 ATPase antibody [EPR7322] (ab129104)
      Other - Anti-SERCA1 ATPase antibody [EPR7322] (ab129104)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    Publishing research using ab129104? Please let us know so that we can cite the reference in this datasheet.

    ab129104 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Western blot abreview for Anti-SERCA1 ATPase antibody [EPR7322]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rabbit Tissue lysate - other (muscle and heart)
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Loading amount
    10 µg
    Specification
    muscle and heart
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Mrs. Minh Hien Le

    Verified customer

    Submitted Feb 10 2016

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