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RecombinantRabMAb

Recombinant Anti-SERCA1 ATPase antibody [EPR7322] - BSA and Azide free (ab248302)

  • Datasheet
  • Certificate of Compliance
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Western blot - Anti-SERCA1 ATPase antibody [EPR7322] - BSA and Azide free (ab248302)
  • OI-RD Scanning - Anti-SERCA1 ATPase antibody [EPR7322] - BSA and Azide free (ab248302)
  • Anti-SERCA1 ATPase antibody [EPR7322] - BSA and Azide free (ab248302)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7322] to SERCA1 ATPase - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

You may also be interested in

Conjugation
Product image
HRP Conjugation Kit - Lightning-Link® (ab102890)
Protein
Product image
Recombinant Human SERCA1 ATPase protein (ab152208)

View more associated products

Overview

  • Product name

    Anti-SERCA1 ATPase antibody [EPR7322] - BSA and Azide free
    See all SERCA1 ATPase primary antibodies
  • Description

    Rabbit monoclonal [EPR7322] to SERCA1 ATPase - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    This antibody can not recognize SERCA1A (ATP2A1A). ab129104 recognizes SERCA1B (ATP2A1B) only.

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human SERCA1 ATPase aa 950-1050. The exact sequence is proprietary.

  • General notes

    Ab248302 is the carrier-free version of ab129104. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab248302 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Dissociation constant (KD)

    KD = 4.50 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR7322
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • ER
    • Signal Transduction
    • Signaling Pathway
    • Calcium Signaling
    • Calcium Channels
    • Signal Transduction
    • Metabolism
    • Plasma Membrane
    • ATPases
    • Cardiovascular
    • Atherosclerosis
    • Hypertension
    • Sodium/ion transport
    • Metabolism
    • Types of disease
    • Heart disease

Associated products

  • Alternative Versions

    • Anti-SERCA1 ATPase antibody [EPR7322] (ab129104)
  • Conjugation kits

    • HRP Conjugation Kit - Lightning-Link® (ab102890)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)
  • Recombinant Protein

    • Recombinant Human SERCA1 ATPase protein (ab152208)

Applications

Our Abpromise guarantee covers the use of ab248302 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 100 kDa (predicted molecular weight: 110 kDa).
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Key regulator of striated muscle performance by acting as the major Ca(2+) ATPase responsible for the reuptake of cytosolic Ca(2+) into the sarcoplasmic reticulum. Catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction.
    • Tissue specificity

      Skeletal muscle, fast twitch muscle (type II) fibers.
    • Involvement in disease

      Brody myopathy
    • Sequence similarities

      Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily.
    • Developmental stage

      Isoform SERCA1A accounts for more than 99% of SERCA1 isoforms expressed in adult skeletal muscle, while isoform SERCA1B predominates in neo-natal skeletal muscle.
    • Cellular localization

      Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane.
    • Target information above from: UniProt accession O14983 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 487 Human
      • Entrez Gene: 11937 Mouse
      • Entrez Gene: 116601 Rat
      • Omim: 108730 Human
      • SwissProt: O14983 Human
      • SwissProt: Q8R429 Mouse
      • SwissProt: Q64578 Rat
      • Unigene: 657344 Human
      • Unigene: 35134 Mouse
      • Unigene: 217139 Rat
      see all
    • Alternative names

      • fast twitch skeletal muscle isoform antibody
      • AT2A1_HUMAN antibody
      • ATP2A antibody
      • ATP2A1 antibody
      • ATPase Ca++ transporting cardiac muscle fast twitch 1 antibody
      • ATPase Ca++ transporting fast twitch 1 antibody
      • ATPase, Ca(2+)-transporting fast twitch 1 antibody
      • Calcium pump 1 antibody
      • Calcium transporting ATPase sarcoplasmic reticulum type fast twitch skeletal muscle isoform antibody
      • Calcium-transporting ATPase sarcoplasmic reticulum type antibody
      • EC 3.6.3.8 antibody
      • Endoplasmic reticulum class 1/2 Ca(2+) ATPase antibody
      • Fast skeletal muscle SR calcium ATPase antibody
      • OTTHUMP00000162561 antibody
      • OTTHUMP00000162562 antibody
      • Sarcoendoplasmic reticulum calcium ATPase antibody
      • Sarcoplasmic reticulum Ca(2+)-ATPase 1 antibody
      • Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 antibody
      • SERCA 1 antibody
      • SERCA1 antibody
      • SERCA1 truncated isoform, included antibody
      • SR Ca(2+) ATPase 1 antibody
      • SR Ca(2+)-ATPase 1 antibody
      see all

    Images

    • Western blot - Anti-SERCA1 ATPase antibody [EPR7322] - BSA and Azide free (ab248302)
      Western blot - Anti-SERCA1 ATPase antibody [EPR7322] - BSA and Azide free (ab248302)
      All lanes : Anti-SERCA1 ATPase antibody [EPR7322] (ab129104) at 1/1000 dilution

      Lane 1 : Human fetal muscle lysate
      Lane 2 : Rat muscle lysate
      Lane 3 : Mouse muscle lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 110 kDa



      This data was developed using ab129104, the same antibody clone in a different buffer formulation.

    • OI-RD Scanning - Anti-SERCA1 ATPase antibody [EPR7322] - BSA and Azide free (ab248302)
      OI-RD Scanning - Anti-SERCA1 ATPase antibody [EPR7322] - BSA and Azide free (ab248302)
      This data was developed using ab129104, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-SERCA1 ATPase antibody [EPR7322] - BSA and Azide free (ab248302)
      Anti-SERCA1 ATPase antibody [EPR7322] - BSA and Azide free (ab248302)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (0)

    Publishing research using ab248302? Please let us know so that we can cite the reference in this datasheet.

    ab248302 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
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    There are currently no Customer reviews or Questions for ab248302.
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