Recombinant Anti-SERCA1 ATPase antibody [EPR7322] - BSA and Azide free (ab248302)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7322] to SERCA1 ATPase - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-SERCA1 ATPase antibody [EPR7322] - BSA and Azide free
See all SERCA1 ATPase primary antibodies -
Description
Rabbit monoclonal [EPR7322] to SERCA1 ATPase - BSA and Azide free -
Host species
Rabbit -
Specificity
This antibody can not recognize SERCA1A (ATP2A1A). ab129104 recognizes SERCA1B (ATP2A1B) only.
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Tested applications
Suitable for: WBmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human SERCA1 ATPase aa 950-1050. The exact sequence is proprietary.
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General notes
Ab248302 is the carrier-free version of ab129104. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab248302 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Dissociation constant (KD)
KD = 4.50 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR7322 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab248302 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | Use at an assay dependent concentration. Detects a band of approximately 100 kDa (predicted molecular weight: 110 kDa). |
Target
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Function
Key regulator of striated muscle performance by acting as the major Ca(2+) ATPase responsible for the reuptake of cytosolic Ca(2+) into the sarcoplasmic reticulum. Catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction. -
Tissue specificity
Skeletal muscle, fast twitch muscle (type II) fibers. -
Involvement in disease
Brody myopathy -
Sequence similarities
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily. -
Developmental stage
Isoform SERCA1A accounts for more than 99% of SERCA1 isoforms expressed in adult skeletal muscle, while isoform SERCA1B predominates in neo-natal skeletal muscle. -
Cellular localization
Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 487 Human
- Entrez Gene: 11937 Mouse
- Entrez Gene: 116601 Rat
- Omim: 108730 Human
- SwissProt: O14983 Human
- SwissProt: Q8R429 Mouse
- SwissProt: Q64578 Rat
- Unigene: 657344 Human
see all -
Alternative names
- fast twitch skeletal muscle isoform antibody
- AT2A1_HUMAN antibody
- ATP2A antibody
see all
Images
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All lanes : Anti-SERCA1 ATPase antibody [EPR7322] (ab129104) at 1/1000 dilution
Lane 1 : Human fetal muscle lysate
Lane 2 : Rat muscle lysate
Lane 3 : Mouse muscle lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 110 kDaThis data was developed using ab129104, the same antibody clone in a different buffer formulation.
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This data was developed using ab129104, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab248302 has not yet been referenced specifically in any publications.