Overview

  • Product name

    Anti-SERCA2 ATPase antibody
    See all SERCA2 ATPase primary antibodies
  • Description

    Goat polyclonal to SERCA2 ATPase
  • Host species

    Goat
  • Specificity

    This antibody is expected to recognize both reported isoforms (NP_001672.1; NP_733765.1).
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit, Pig
  • Immunogen

    Synthetic peptide corresponding to Human SERCA2 ATPase aa 658-671 (internal sequence).
    Sequence:

    DELNPSAQRDACLN


    Database link: P16615

  • Positive control

    • IHC-P: Human heart tissue. WB: Human, mouse and rat heart lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab219173 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 115 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Isoform 2 is involved in the regulation of the contraction/relaxation cycle.
  • Tissue specificity

    Isoform 1 is widely expressed in smooth muscle and nonmuscle tissues such as in adult skin epidermis, with highest expression in liver, pancreas and lung, and intermediate expression in brain, kidney and placenta. Also expressed at lower levels in heart and skeletal muscle. Isoforms 2 and 3 are highly expressed in the heart and slow twitch skeletal muscle. Expression of isoform 3 is predominantly restricted to cardiomyocytes and in close proximity to the sarcolemma. Both isoforms are mildly expressed in lung, kidney, liver, pancreas and placenta. Expression of isoform 3 is amplified during monocytic differentiation and also observed in the fetal heart.
  • Involvement in disease

    Defects in ATP2A2 are a cause of acrokeratosis verruciformis (AKV) [MIM:101900]; also known as Hopf disease. AKV is a localized disorder of keratinization, which is inherited as an autosomal dominant trait. Its onset is early in life with multiple flat-topped, flesh-colored papules on the hands and feet, punctate keratoses on the palms and soles, with varying degrees of nail involvement. The histopathology shows a distinctive pattern of epidermal features with hyperkeratosis, hypergranulosis, and acanthosis together with papillomatosis. These changes are frequently associated with circumscribed elevations of the epidermis that are said to resemble church spires. There are no features of dyskeratosis or acantholysis, the typical findings in lesions of Darier disease.
    Defects in ATP2A2 are the cause of Darier disease (DD) [MIM:124200]; also known as Darier-White disease (DAR). DD is an autosomal dominantly inherited skin disorder characterized by loss of adhesion between epidermal cells (acantholysis) and abnormal keratinization. Patients with mild disease may have no more than a few scattered keratotic papules or subtle nail changes, whereas those with severe disease are handicapped by widespread malodorous keratotic plaques. In a few families, neuropsychiatric abnormalities such as mild mental retardation, schizophrenia, bipolar disorder and epilepsy have been reported. Stress, UV exposure, heat, sweat, friction, and oral contraception exacerbate disease symptoms. Prevalence has been estimated at 1 in 50000. Clinical variants of DD include hypertrophic, vesicobullous, hypopigmented, cornifying, zosteriform or linear, acute and comedonal subtypes. Comedonal Darier disease (CDD) is characterized by the coexistence of acne-like comedonal lesions with typical Darier hyperkeratotic papules on light-exposed areas. At histopathologic level, CDD differs from classic DD in the prominent follicular involvement and the presence of greatly elongated dermal villi.
  • Sequence similarities

    Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily.
  • Post-translational
    modifications

    Nitrated under oxidative stress. Nitration on the two tyrosine residues inhibits catalytic activity.
  • Cellular localization

    Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • AT2A2_HUMAN antibody
    • Atp2a2 antibody
    • ATP2B antibody
    • ATPase Ca++ transporting cardiac muscle slow twitch 2 antibody
    • Calcium pump 2 antibody
    • Calcium-transporting ATPase sarcoplasmic reticulum type antibody
    • Calcium-transporting ATPase sarcoplasmic reticulum type slow twitch skeletal muscle isoform antibody
    • Cardiac Ca2+ ATPase antibody
    • DAR antibody
    • DD antibody
    • Endoplasmic reticulum class 1/2 Ca(2+) ATPase antibody
    • MGC45367 antibody
    • Sarcoplasmic/endoplasmic reticulum calcium ATPase 2 antibody
    • SERCA 2 antibody
    • SERCA2 antibody
    • serca2a antibody
    • slow twitch skeletal muscle isoform antibody
    • SR Ca(2+)-ATPase 2 antibody
    see all

Images

  • All lanes : Anti-SERCA2 ATPase antibody (ab219173) at 0.1 µg/ml

    Lane 1 : Human heart lysate, 35 µg in RIPA buffer
    Lane 2 : Mouse heart lysate, 35 µg in RIPA buffer
    Lane 3 : Rat heart lysate, 35 ug in RIPA buffer

    Developed using the ECL technique.

    Predicted band size: 115 kDa



    Primary incubation for 1 hour.

  • Immunohistochemical analysis of formalin-fixed paraffin-embedded human heart tissue sections labeling SERCA2 ATPase with ab219173 at 5 µg/mL.

References

ab219173 has not yet been referenced specifically in any publications.

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