Western blot abreview for Anti-SERCA2 ATPase antibody

Average
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (Heart (Ventricle))
Loading amount
50 µg
Specification
Heart (Ventricle)
Gel Running Conditions
Reduced Denaturing (8% polyacrylamide gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/25000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 1% Milk TBS-T

Secondary antibody

Name
Non-Abcam antibody was used: Jackson Immunological Anti-Rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000

Detection

Detection method
ECL
Exposure
1 minute(s) and 30 second(s)
Bands
Specific: 110 kDa Non-specific: 64, 148? kDa

Additional data

Additional Notes
Samples 1-4: Wild Type Mouse Heart Lysate

We tried using multiple dilutions of primary antibody ranging from 1:1000-1:25000. We found that using higher antibody concentrations gave us very dark uninterpretable blots.

Dr. Serban Georgescu

Verified customer

Submitted Jun 04 2008

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