Western blot abreview for Anti-SERCA2 ATPase antibody

Good
Abreviews
Application
Western blot
Sample
Pig Tissue lysate - whole (heart)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
1.5 µg
Specification
heart
Blocking step
(agent) for 2 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 4°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Dilution
1/20000

Secondary antibody

Dilution
1/2000
Name
Non-Abcam antibody was used:
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer

Detection

Detection method
ECL
Exposure
10 second(s)
Bands
Specific: 115 kDa
Positive control
Normal heart tissue

Additional data

Additional Notes
N= normal region of a MI heart P= peri-infract region of a MI heart MI= myocardial infract region of a MI heart

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Submitted Jun 06 2016

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