RecombinantRabMAb

Recombinant Anti-SERCA2 ATPase antibody [EPR9392] (ab150435)

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Western blot - Anti-SERCA2 ATPase antibody [EPR9392] (ab150435)
  • Immunocytochemistry/ Immunofluorescence - Anti-SERCA2 ATPase antibody [EPR9392] (ab150435)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA2 ATPase antibody [EPR9392] (ab150435)
  • Flow Cytometry - Anti-SERCA2 ATPase antibody [EPR9392] (ab150435)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA2 ATPase antibody [EPR9392] (ab150435)
  • Immunocytochemistry/ Immunofluorescence - Anti-SERCA2 ATPase antibody [EPR9392] (ab150435)
  • Flow Cytometry - Anti-SERCA2 ATPase antibody [EPR9392] (ab150435)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA2 ATPase antibody [EPR9392] (ab150435)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA2 ATPase antibody [EPR9392] (ab150435)
  • Anti-SERCA2 ATPase antibody [EPR9392] (ab150435)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9392] to SERCA2 ATPase
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SERCA2 ATPase antibody [EPR9392]
    See all SERCA2 ATPase primary antibodies

  • Description

    Rabbit monoclonal [EPR9392] to SERCA2 ATPase

  • Host species

    Rabbit

  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human SERCA2 ATPase aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • WB: HeLa and HepG2 whole cell lysates. Rat and mouse brain tissue lysates. ICC/IF: HeLa and HepG2 cells. IHC-P: Human kidney, liver, brain, and lung tissue. Flow-cyt: HepG2 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab150435 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
ICC/IF
Human
IHC-P
Human
WB
Mouse
Rat
Human
Application Abreviews Notes
WB
1/5000 - 1/10000. Predicted molecular weight: 115 kDa.

For unpurified use at 1/20000 - 1/100000.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
ICC/IF
1/100 - 1/250.
Flow Cyt
1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Notes
WB
1/5000 - 1/10000. Predicted molecular weight: 115 kDa.

For unpurified use at 1/20000 - 1/100000.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
ICC/IF
1/100 - 1/250.
Flow Cyt
1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function

    This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Isoform 2 is involved in the regulation of the contraction/relaxation cycle.

  • Tissue specificity

    Isoform 1 is widely expressed in smooth muscle and nonmuscle tissues such as in adult skin epidermis, with highest expression in liver, pancreas and lung, and intermediate expression in brain, kidney and placenta. Also expressed at lower levels in heart and skeletal muscle. Isoforms 2 and 3 are highly expressed in the heart and slow twitch skeletal muscle. Expression of isoform 3 is predominantly restricted to cardiomyocytes and in close proximity to the sarcolemma. Both isoforms are mildly expressed in lung, kidney, liver, pancreas and placenta. Expression of isoform 3 is amplified during monocytic differentiation and also observed in the fetal heart.

  • Involvement in disease

    Defects in ATP2A2 are a cause of acrokeratosis verruciformis (AKV) [MIM:101900]; also known as Hopf disease. AKV is a localized disorder of keratinization, which is inherited as an autosomal dominant trait. Its onset is early in life with multiple flat-topped, flesh-colored papules on the hands and feet, punctate keratoses on the palms and soles, with varying degrees of nail involvement. The histopathology shows a distinctive pattern of epidermal features with hyperkeratosis, hypergranulosis, and acanthosis together with papillomatosis. These changes are frequently associated with circumscribed elevations of the epidermis that are said to resemble church spires. There are no features of dyskeratosis or acantholysis, the typical findings in lesions of Darier disease.
    Defects in ATP2A2 are the cause of Darier disease (DD) [MIM:124200]; also known as Darier-White disease (DAR). DD is an autosomal dominantly inherited skin disorder characterized by loss of adhesion between epidermal cells (acantholysis) and abnormal keratinization. Patients with mild disease may have no more than a few scattered keratotic papules or subtle nail changes, whereas those with severe disease are handicapped by widespread malodorous keratotic plaques. In a few families, neuropsychiatric abnormalities such as mild mental retardation, schizophrenia, bipolar disorder and epilepsy have been reported. Stress, UV exposure, heat, sweat, friction, and oral contraception exacerbate disease symptoms. Prevalence has been estimated at 1 in 50000. Clinical variants of DD include hypertrophic, vesicobullous, hypopigmented, cornifying, zosteriform or linear, acute and comedonal subtypes. Comedonal Darier disease (CDD) is characterized by the coexistence of acne-like comedonal lesions with typical Darier hyperkeratotic papules on light-exposed areas. At histopathologic level, CDD differs from classic DD in the prominent follicular involvement and the presence of greatly elongated dermal villi.

  • Sequence similarities

    Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily.

  • Post-translational
    modifications

    Nitrated under oxidative stress. Nitration on the two tyrosine residues inhibits catalytic activity.

  • Cellular localization

    Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • AT2A2_HUMAN antibody
    • Atp2a2 antibody
    • ATP2B antibody
    • ATPase Ca++ transporting cardiac muscle slow twitch 2 antibody
    • Calcium pump 2 antibody
    • Calcium-transporting ATPase sarcoplasmic reticulum type antibody
    • Calcium-transporting ATPase sarcoplasmic reticulum type slow twitch skeletal muscle isoform antibody
    • Cardiac Ca2+ ATPase antibody
    • DAR antibody
    • DD antibody
    • Endoplasmic reticulum class 1/2 Ca(2+) ATPase antibody
    • MGC45367 antibody
    • Sarcoplasmic/endoplasmic reticulum calcium ATPase 2 antibody
    • SERCA 2 antibody
    • SERCA2 antibody
    • serca2a antibody
    • slow twitch skeletal muscle isoform antibody
    • SR Ca(2+)-ATPase 2 antibody
    see all

Images

  • Western blot - Anti-SERCA2 ATPase antibody [EPR9392] (ab150435)
    Western blot - Anti-SERCA2 ATPase antibody [EPR9392] (ab150435)
    All lanes : Anti-SERCA2 ATPase antibody [EPR9392] (ab150435) at 1/5000 dilution (Purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates boiled
    Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates unboiled
    Lane 3 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates boiled
    Lane 4 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates unboiled
    Lane 5 : Mouse brain lysates boiled
    Lane 6 : Mouse brain lysates unboiled
    Lane 7 : Rat brain lysates boiled
    Lane 8 : Rat brain lysates unboiled

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 115 kDa
    Observed band size: 115,140 kDa
    why is the actual band size different from the predicted?



    Blocking/Diluting buffer: 5% NFDM/TBST

    Suggest to use non-boiled samples, as boiling process could cause membrane protein aggregates (PMID: 16023741 and PMID: 8670158).

  • Immunocytochemistry/ Immunofluorescence - Anti-SERCA2 ATPase antibody [EPR9392] (ab150435)
    Immunocytochemistry/ Immunofluorescence - Anti-SERCA2 ATPase antibody [EPR9392] (ab150435)

    Immunocytochemistry/ Immunofluorescence analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling SERCA2 ATPase with Purified ab150435 at 1/100 dilution (10 µg/mL). Cells were fixed in 100% Methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA2 ATPase antibody [EPR9392] (ab150435)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA2 ATPase antibody [EPR9392] (ab150435)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung carcinoma tissue sections labeling SERCA2 ATPase with purified ab150435 at 1/50 dilution (20 µg/mL). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Flow Cytometry - Anti-SERCA2 ATPase antibody [EPR9392] (ab150435)
    Flow Cytometry - Anti-SERCA2 ATPase antibody [EPR9392] (ab150435)
    Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling SERCA2 ATPase with Purified ab150435 at 1/1000 dilution (1 µg/mL) (Red). Cells were fixed with 80% Methanol and permeabilised with 0.1% Tween-20. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA2 ATPase antibody [EPR9392] (ab150435)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA2 ATPase antibody [EPR9392] (ab150435)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human brain tissue sections labeling SERCA2 ATPase with purified ab150435 at 1/50 dilution (20 µg/mL). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunocytochemistry/ Immunofluorescence - Anti-SERCA2 ATPase antibody [EPR9392] (ab150435)
    Immunocytochemistry/ Immunofluorescence - Anti-SERCA2 ATPase antibody [EPR9392] (ab150435)

    Immunofluorescence analysis of HeLa cells labelling SERCA2 ATPase with unpurified ab150435 at 1/100.

  • Flow Cytometry - Anti-SERCA2 ATPase antibody [EPR9392] (ab150435)
    Flow Cytometry - Anti-SERCA2 ATPase antibody [EPR9392] (ab150435)

    Overlay histogram showing HepG2 cells stained with unpurified ab150435 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab150435, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA2 ATPase antibody [EPR9392] (ab150435)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA2 ATPase antibody [EPR9392] (ab150435)

    Immunohistochemical analysis of paraffin embedded Human liver tissue labelling SERCA2 ATPase with unpurified ab150435 at 1/50.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA2 ATPase antibody [EPR9392] (ab150435)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA2 ATPase antibody [EPR9392] (ab150435)

    Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling SERCA2 ATPase with unpurified ab150435 at 1/50.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-SERCA2 ATPase antibody [EPR9392] (ab150435)
    Anti-SERCA2 ATPase antibody [EPR9392] (ab150435)

References (4)

Publishing research using ab150435? Please let us know so that we can cite the reference in this datasheet.

ab150435 has been referenced in 4 publications.

  • Roczkowsky A  et al. Myocardial MMP-2 contributes to SERCA2a proteolysis during cardiac ischemia-reperfusion injury. Cardiovasc Res N/A:N/A (2019). PubMed: 31373602
  • Löser S  et al. Pulmonary ORMDL3 is critical for induction of Alternaria-induced allergic airways disease. J Allergy Clin Immunol 139:1496-1507.e3 (2017). WB ; Human . PubMed: 27623174
  • Zhong P  et al. CaMKII Activation Promotes Cardiac Electrical Remodeling and Increases the Susceptibility to Arrhythmia Induction in High-fat Diet-Fed Mice With Hyperlipidemia Conditions. J Cardiovasc Pharmacol 70:245-254 (2017). PubMed: 28662005
  • Wu J  et al. Gp130-mediated STAT3 activation by S-propargyl-cysteine, an endogenous hydrogen sulfide initiator, prevents doxorubicin-induced cardiotoxicity. Cell Death Dis 7:e2339 (2016). PubMed: 27537522

Customer reviews and Q&As

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Immunocytochemistry abreview for Anti-SERCA2 ATPase antibody [EPR9392]

 Good
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Mouse Cultured Cells (Cardiomyocytes)
Permeabilization
Yes - Triton x-100, 0.01%
Specification
Cardiomyocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde
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Submitted Jan 15 2018

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