Recombinant
RabMAb

Recombinant Anti-SERCA2 ATPase antibody [EPR9392] - BSA and Azide free (ab238426)

Overview

  • Product name

    Anti-SERCA2 ATPase antibody [EPR9392] - BSA and Azide free
    See all SERCA2 ATPase primary antibodies
  • Description

    Rabbit monoclonal [EPR9392] to SERCA2 ATPase - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IF, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human SERCA2 ATPase aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • IHC-P: Human kidney and liver tissues. ICC/IF: HeLa cells. Flow Cyt: HepG2 cells.
  • General notes

    Ab238426 is the carrier-free version of ab150435. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab238426 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab238426 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 115 kDa.
Flow Cyt Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Isoform 2 is involved in the regulation of the contraction/relaxation cycle.
    • Tissue specificity

      Isoform 1 is widely expressed in smooth muscle and nonmuscle tissues such as in adult skin epidermis, with highest expression in liver, pancreas and lung, and intermediate expression in brain, kidney and placenta. Also expressed at lower levels in heart and skeletal muscle. Isoforms 2 and 3 are highly expressed in the heart and slow twitch skeletal muscle. Expression of isoform 3 is predominantly restricted to cardiomyocytes and in close proximity to the sarcolemma. Both isoforms are mildly expressed in lung, kidney, liver, pancreas and placenta. Expression of isoform 3 is amplified during monocytic differentiation and also observed in the fetal heart.
    • Involvement in disease

      Defects in ATP2A2 are a cause of acrokeratosis verruciformis (AKV) [MIM:101900]; also known as Hopf disease. AKV is a localized disorder of keratinization, which is inherited as an autosomal dominant trait. Its onset is early in life with multiple flat-topped, flesh-colored papules on the hands and feet, punctate keratoses on the palms and soles, with varying degrees of nail involvement. The histopathology shows a distinctive pattern of epidermal features with hyperkeratosis, hypergranulosis, and acanthosis together with papillomatosis. These changes are frequently associated with circumscribed elevations of the epidermis that are said to resemble church spires. There are no features of dyskeratosis or acantholysis, the typical findings in lesions of Darier disease.
      Defects in ATP2A2 are the cause of Darier disease (DD) [MIM:124200]; also known as Darier-White disease (DAR). DD is an autosomal dominantly inherited skin disorder characterized by loss of adhesion between epidermal cells (acantholysis) and abnormal keratinization. Patients with mild disease may have no more than a few scattered keratotic papules or subtle nail changes, whereas those with severe disease are handicapped by widespread malodorous keratotic plaques. In a few families, neuropsychiatric abnormalities such as mild mental retardation, schizophrenia, bipolar disorder and epilepsy have been reported. Stress, UV exposure, heat, sweat, friction, and oral contraception exacerbate disease symptoms. Prevalence has been estimated at 1 in 50000. Clinical variants of DD include hypertrophic, vesicobullous, hypopigmented, cornifying, zosteriform or linear, acute and comedonal subtypes. Comedonal Darier disease (CDD) is characterized by the coexistence of acne-like comedonal lesions with typical Darier hyperkeratotic papules on light-exposed areas. At histopathologic level, CDD differs from classic DD in the prominent follicular involvement and the presence of greatly elongated dermal villi.
    • Sequence similarities

      Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily.
    • Post-translational
      modifications

      Nitrated under oxidative stress. Nitration on the two tyrosine residues inhibits catalytic activity.
    • Cellular localization

      Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • AT2A2_HUMAN antibody
      • Atp2a2 antibody
      • ATP2B antibody
      • ATPase Ca++ transporting cardiac muscle slow twitch 2 antibody
      • Calcium pump 2 antibody
      • Calcium-transporting ATPase sarcoplasmic reticulum type antibody
      • Calcium-transporting ATPase sarcoplasmic reticulum type slow twitch skeletal muscle isoform antibody
      • Cardiac Ca2+ ATPase antibody
      • DAR antibody
      • DD antibody
      • Endoplasmic reticulum class 1/2 Ca(2+) ATPase antibody
      • MGC45367 antibody
      • Sarcoplasmic/endoplasmic reticulum calcium ATPase 2 antibody
      • SERCA 2 antibody
      • SERCA2 antibody
      • serca2a antibody
      • slow twitch skeletal muscle isoform antibody
      • SR Ca(2+)-ATPase 2 antibody
      see all

    Images

    • Overlay histogram showing HepG2 cells stained with ab150435 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab150435, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150435).

    • Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling SERCA2 ATPase with ab150435 at 1/50.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150435).

    • Immunohistochemical analysis of paraffin embedded Human liver tissue labelling SERCA2 ATPase with ab150435 at 1/50.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150435).

    • Immunofluorescence analysis of HeLa cells labelling SERCA2 ATPase with ab150435 at 1/100.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150435).

    References

    ab238426 has not yet been referenced specifically in any publications.

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