Overview

  • Product name
    Anti-SERCA3 ATPase antibody
    See all SERCA3 ATPase primary antibodies
  • Description
    Rabbit polyclonal to SERCA3 ATPase
  • Host species
    Rabbit
  • Specificity
    Detects sarcoplasmic or endoplasmic reticulum calcium ATPase 3 (SERC A3) human and rat recombinant protein. This antibody does not detect other SERCA isoforms.
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Dog, Human
  • Immunogen

    Synthetic peptide corresponding to Mouse SERCA3 ATPase aa 29-39.
    Sequence:

    VTDARERYGPN


    (Peptide available as ab4955)

Properties

Applications

Our Abpromise guarantee covers the use of ab3339 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 97 kDa (predicted molecular weight: 113 kDa).Can be blocked with SERCA3 ATPase peptide (ab4955). By Western blot, this antibody detects an ~97 kDa protein representing recombinant SERCA3 from rat samples.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium. Transports calcium ions from the cytosol into the sarcoplasmic/endoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction.
  • Tissue specificity
    Found in most tissues. Most abundant in thymus, trachea, salivary gland, spleen, bone marrow, lymph node, peripheral leukocytes, pancreas and colon. Also detected in fetal tissues.
  • Sequence similarities
    Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily.
  • Cellular localization
    Nucleus membrane. Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Adenosine triphosphatase calcium antibody
    • AT2A3_HUMAN antibody
    • Atp2a3 antibody
    • ATPase Ca(2+) transporting ubiquitous antibody
    • ATPase Ca++ transporting ubiquitous antibody
    • Calcium pump 3 antibody
    • Calcium translocating P type ATPase antibody
    • Sarco/endoplasmic reticulum Ca2+ ATPase antibody
    • Sarcoplasmic/endoplasmic reticulum calcium ATPase 3 antibody
    • SERCA 3 antibody
    • SERCA3 antibody
    • SERCA3b antibody
    • SR Ca(2+) ATPase 3 antibody
    • SR Ca(2+)-ATPase 3 antibody
    see all

Images

  • Western blot of recombinant human SERCA3 ATPase expressed in E. coli using ab3339. Western blot of recombinant human SERCA3 ATPase expressed in E. coli using ab3339.

References

This product has been referenced in:
  • Poch E  et al. Functional characterization of alternatively spliced human SERCA3 transcripts. Am J Physiol 275:C1449-58 (1998). Read more (PubMed: 9843705) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (HEK 293)
Loading amount
30 µg
Specification
HEK 293
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Aug 15 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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