Key features and details
- Rabbit polyclonal to SERCA3 ATPase - N-terminal
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-SERCA3 ATPase antibody - N-terminal
DescriptionRabbit polyclonal to SERCA3 ATPase - N-terminal
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow, Xenopus laevis, Xenopus tropicalis
Recombinant fragment, corresponding to a region within N -terminal amino acids 3-185 of Human SERCA3 ATPase (Uniprot ID: Q93084).
- Jurkat, Raji, K562 and BCL1 whole cell lysates; Human colon carcinoma tissue.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab154259 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 114 kDa.|
|IHC-P||1/100 - 1/1000.|
FunctionThis magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium. Transports calcium ions from the cytosol into the sarcoplasmic/endoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction.
Tissue specificityFound in most tissues. Most abundant in thymus, trachea, salivary gland, spleen, bone marrow, lymph node, peripheral leukocytes, pancreas and colon. Also detected in fetal tissues.
Sequence similaritiesBelongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily.
Cellular localizationNucleus membrane. Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane.
- Information by UniProt
- Adenosine triphosphatase calcium antibody
- AT2A3_HUMAN antibody
- Atp2a3 antibody
All lanes : Anti-SERCA3 ATPase antibody - N-terminal (ab154259) at 1/2000 dilution
Lane 1 : Jurkat whole cell lysate
Lane 2 : Raji whole cell lysate
Lane 3 : K562 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 114 kDa
5% SDS PAGE
Anti-SERCA3 ATPase antibody - N-terminal (ab154259) at 1/5000 dilution + BCL1 whole cell lysate at 30 µg
Predicted band size: 114 kDa
5% SDS PAGE
Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling SERCA3 ATPase with ab154259 at 1/500 dilution.
ab154259 has not yet been referenced specifically in any publications.