Key features and details
- Rabbit polyclonal to Serglycin
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Product nameAnti-Serglycin antibody
DescriptionRabbit polyclonal to Serglycin
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse
Predicted to work with: Rat
Synthetic peptide corresponding to Rat Serglycin aa 1-100 conjugated to keyhole limpet haemocyanin.
Database link: P04917
- This antibody gave a positive signal in the following Mouse tissue lysates: White Adipose Tissue from Subcutaneous & Visceral Fat - Fasted; White Adipose Tissue from Gonad - High Fat Diet (Male); White Adipose Tissue from Gonad - High Fat Diet (Female).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab156991 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).|
FunctionPlays a role in formation of mast cell secretory granules and mediates storage of various compounds in secretory vesicles. Required for storage of some proteases in both connective tissue and mucosal mast cells and for storage of granzyme B in T lymphocytes. Plays a role in localizing neutrophil elastase in azurophil granules of neutrophils. Mediates processing of MMP2. Plays a role in cytotoxic cell granule-mediated apoptosis by forming a complex with granzyme B which is delivered to cells by perforin to induce apoptosis. Regulates the secretion of TNF-alpha and may also regulate protease secretion. Inhibits bone mineralization.
Sequence similaritiesBelongs to the serglycin family.
modificationsO-glcosylated; contains chondroitin sulfate and heparan sulfate.
Cellular localizationCytoplasmic granule. Secreted > extracellular space. Golgi apparatus. Found in mast cell granules and in cytoplasmic granules of cytolytic T lymphocytes from where it is secreted upon cell activation. Secreted constitutively by endothelial cells and macrophages. Located to Golgi apparatus during neutrophil differentiation.
- Information by UniProt
- Chondroitin sulfate proteoglycan core protein antibody
- Cytolytic granule proteoglycan core protein antibody
- FLJ12930 antibody
All lanes : Anti-Serglycin antibody (ab156991) at 1 µg/ml
Lane 1 : Mouse WAT (White Adipose Tissue) (Fasted) Tissue Lysate
Lane 2 : Mouse (Male) WAT from Gonad (High Fat Diet) Tissue Lysate
Lane 3 : Mouse (Female) WAT from Gonad (High Fat Diet) Tissue Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 18 kDa
Observed band size: 18 kDa
Additional bands at: 14 kDa (possible non-specific binding), 30 kDa (possible non-specific binding)
Exposure time: 8 minutes
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab156991 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
ab156991 has not yet been referenced specifically in any publications.