Overview

  • Product name
  • Description
    Rabbit polyclonal to Serglycin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to Rat Serglycin aa 1-100 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: P04917

  • Positive control
    • This antibody gave a positive signal in the following Mouse tissue lysates: White Adipose Tissue from Subcutaneous & Visceral Fat - Fasted; White Adipose Tissue from Gonard - High Fat Diet (Male); White Adipose Tissue from Gonard - High Fat Diet (Female).

Properties

Applications

Our Abpromise guarantee covers the use of ab156991 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).

Target

  • Function
    Plays a role in formation of mast cell secretory granules and mediates storage of various compounds in secretory vesicles. Required for storage of some proteases in both connective tissue and mucosal mast cells and for storage of granzyme B in T lymphocytes. Plays a role in localizing neutrophil elastase in azurophil granules of neutrophils. Mediates processing of MMP2. Plays a role in cytotoxic cell granule-mediated apoptosis by forming a complex with granzyme B which is delivered to cells by perforin to induce apoptosis. Regulates the secretion of TNF-alpha and may also regulate protease secretion. Inhibits bone mineralization.
  • Sequence similarities
    Belongs to the serglycin family.
  • Post-translational
    modifications
    O-glcosylated; contains chondroitin sulfate and heparan sulfate.
  • Cellular localization
    Cytoplasmic granule. Secreted > extracellular space. Golgi apparatus. Found in mast cell granules and in cytoplasmic granules of cytolytic T lymphocytes from where it is secreted upon cell activation. Secreted constitutively by endothelial cells and macrophages. Located to Golgi apparatus during neutrophil differentiation.
  • Information by UniProt
  • Database links
  • Alternative names
    • Chondroitin sulfate proteoglycan core protein antibody
    • Cytolytic granule proteoglycan core protein antibody
    • FLJ12930 antibody
    • gp600 antibody
    • Hematopoetic proteoglycan core protein antibody
    • Mastocytoma proteoglycan core protein antibody
    • MGC9289 antibody
    • OTTHUMP00000019716 antibody
    • P.PG antibody
    • PG19 core protein antibody
    • Pgsg antibody
    • Platelet proteoglycan core protein antibody
    • platelet proteoglycan protein core antibody
    • PLATELET PROTEOGLYCAN PROTEIN CORE; PPG antibody
    • PPG antibody
    • PRG antibody
    • PRG1 antibody
    • PROTEOGLYCAN 1 antibody
    • proteoglycan 1, secretory granule antibody
    • Proteoglycan 10K core protein antibody
    • Proteoglycan peptide core protein antibody
    • PROTEOGLYCAN PROTEIN CORE FOR MAST CELL SECRETORY GRANULE antibody
    • secretory granule proteoglycan 1 antibody
    • Secretory granule proteoglycan core peptide antibody
    • Secretory granule proteoglycan core protein antibody
    • Serglycin antibody
    • serglycin proteoglycan antibody
    • Sgc antibody
    • Srgn antibody
    • SRGN_HUMAN antibody
    see all

Images

  • All lanes : Anti-Serglycin antibody (ab156991) at 1 µg/ml

    Lane 1 : Mouse WAT (White Adipose Tissue) (Fasted) Tissue Lysate
    Lane 2 : Mouse (Male) WAT from Gonard (High Fat Diet) Tissue Lysate
    Lane 3 : Mouse (Female) WAT from Gonard (High Fat Diet) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 18 kDa
    Observed band size: 18 kDa
    Additional bands at: 14 kDa (possible non-specific binding), 30 kDa (possible non-specific binding)


    Exposure time: 8 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab156991 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

ab156991 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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