Anti-SERINC5 antibody (ab204400)

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Overview

  • Product name

    Anti-SERINC5 antibody

  • Description

    Rabbit polyclonal to SERINC5

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cynomolgus monkey

  • Immunogen

    Recombinant fragment corresponding to Human SERINC5 aa 335-385.
    Sequence:

    RSSSDALQGRYAAPELEIARCCFCFSPGGEDTEEQQPGKEGPRVIYDEKK G


    Database link: Q86VE9

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Human esophagus tissue; Human tonsil tissue lysate; U-2OS cells.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituents: 40% Glycerol, 59% PBS
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab204400 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/250. Predicted molecular weight: 47 kDa.
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Target

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERINC5 antibody (ab204400)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERINC5 antibody (ab204400)

    Immunohistochemical analysis of paraffin-embedded Human esophagus tissue labeling SERINC5 with ab204400 antibody at a dilution of 1/500.

  • Western blot - Anti-SERINC5 antibody (ab204400)
    Western blot - Anti-SERINC5 antibody (ab204400)
    All lanes : Anti-SERINC5 antibody (ab204400) at 1/100 dilution

    Lane 1 : Molecular weight marker
    Lane 2 : RT-4 cell lysate
    Lane 3 : U-251 MG cell lysate
    Lane 4 : Human Plasma
    Lane 5 : Human liver tissue lysate
    Lane 6 : Human tonsil tissue lysate

    Developed using the ECL technique.

    Predicted band size: 47 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-SERINC5 antibody (ab204400)
    Immunocytochemistry/ Immunofluorescence - Anti-SERINC5 antibody (ab204400)

    Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized U-2 OS cells labeling SERINC5 with ab204400 at 4 µg/mL (green).

References

ab204400 has not yet been referenced specifically in any publications.

Publishing research using ab204400? Please let us know so that we can cite the reference in this datasheet.

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Immunohistochemistry (Frozen sections) abreview for Anti-SERINC5 antibody

 Average
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Permeabilization
Yes - Triton X-100
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
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Verified customer

Submitted Jun 07 2016

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