Anti-SERINC5 antibody (ab204400)
Key features and details
- Rabbit polyclonal to SERINC5
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SERINC5 antibody -
Description
Rabbit polyclonal to SERINC5 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cynomolgus monkey -
Immunogen
Recombinant fragment corresponding to Human SERINC5 aa 300-400.
Database link: Q86VE9 -
Positive control
- Human esophagus tissue; Human tonsil tissue lysate; U-2OS cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab204400 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/100 - 1/250. Predicted molecular weight: 47 kDa.
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IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
Use a concentration of 1 - 4 µg/ml.
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Notes |
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WB
1/100 - 1/250. Predicted molecular weight: 47 kDa. |
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 1 - 4 µg/ml. |
Target
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Function
Enhances the incorporation of serine into phosphatidylserine and sphingolipids. May play a role in providing serine molecules for the formation of myelin glycosphingolipids in oligodendrocytes. -
Tissue specificity
Highly expressed in placenta, skeletal muscle, spleen, thymus, testis and peripheral leukocyte and is expressed weakly in the heart, liver and fetal brain. -
Sequence similarities
Belongs to the TDE1 family. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 101925282 Cynomolgus monkey
- Entrez Gene: 256987 Human
- Entrez Gene: 218442 Mouse
- Entrez Gene: 170907 Rat
- Omim: 614551 Human
- SwissProt: Q4R6L9 Cynomolgus monkey
- SwissProt: Q86VE9 Human
- SwissProt: Q63175 Mouse
see all -
Alternative names
- C5orf12 antibody
- SERC5_HUMAN antibody
- SERINC5 antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded Human esophagus tissue labeling SERINC5 with ab204400 antibody at a dilution of 1/500.
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All lanes : Anti-SERINC5 antibody (ab204400) at 1/100 dilution
Lane 1 : Molecular weight marker
Lane 2 : RT-4 cell lysate
Lane 3 : U-251 MG cell lysate
Lane 4 : Human Plasma
Lane 5 : Human liver tissue lysate
Lane 6 : Human tonsil tissue lysate
Developed using the ECL technique.
Predicted band size: 47 kDa -
Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized U-2 OS cells labeling SERINC5 with ab204400 at 4 µg/mL (green).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab204400 has been referenced in 3 publications.
- Ahi YS et al. IFITM3 Reduces Retroviral Envelope Abundance and Function and Is Counteracted by glycoGag. mBio 11:N/A (2020). PubMed: 31964738
- Li W et al. Antiviral Role of Serine Incorporator 5 (SERINC5) Proteins in Classical Swine Fever Virus Infection. Front Microbiol 11:580233 (2020). PubMed: 33013817
- Jin SW et al. Natural HIV-1 Nef Polymorphisms Impair SERINC5 Downregulation Activity. Cell Rep 29:1449-1457.e5 (2019). PubMed: 31693887