Overview

  • Product name
    Anti-Serine Palmitoyltransferase antibody
    See all Serine Palmitoyltransferase primary antibodies
  • Description
    Rabbit polyclonal to Serine Palmitoyltransferase
  • Host species
    Rabbit
  • Specificity
    This antibody detects human serine palmitoyltransferase subunit SPT2
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Chicken, Cow, Human, Pig, Xenopus laevis, Zebrafish, African green monkey
  • Immunogen

    Synthetic peptide:

    LDRPFDETTYEETED

    , corresponding to amino acids 548-562 of Human Serine Palmitoyltransferase.

  • Positive control
    • Mouse brain homogenate, rat kidney supernatant, human liver microsome.

Properties

Applications

Our Abpromise guarantee covers the use of ab23696 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 62 kDa.
IHC-P Use a concentration of 1 µg/ml.

Target

  • Function
    Serine palmitoyltransferase (SPT). The heterodimer formed with LCB1/SPTLC1 constitutes the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC2-SSSPTA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC2-SSSPTB complex displays a preference for C18-CoA substrate.
  • Tissue specificity
    Widely expressed.
  • Pathway
    Lipid metabolism; sphingolipid metabolism.
  • Involvement in disease
    Defects in SPTLC2 are the cause of hereditary sensory and autonomic neuropathy type 1C (HSAN1C) [MIM:613640]. It is a form of hereditary sensory and autonomic neuropathy, a genetically and clinically heterogeneous group of disorders characterized by degeneration of dorsal root and autonomic ganglion cells, and by prominent sensory abnormalities with a variable degree of motor and autonomic dysfunction. The neurological phenotype is often complicated by severe infections, osteomyelitis, and amputations. HSAN1C symptoms include loss of touch and vibration in the feet, dysesthesia and severe panmodal sensory loss in the upper and lower limbs, distal lower limb sensory loss with ulceration and osteomyelitis, and distal muscle weakness.
  • Sequence similarities
    Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.
  • Cellular localization
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • KIAA0526 antibody
    • LCB 2 antibody
    • LCB2 antibody
    • LCB2a antibody
    • Long chain base biosynthesis protein 2 antibody
    • Long chain base biosynthesis protein 2a antibody
    • Serine palmitoyl CoA transferase 2 antibody
    • Serine palmitoyltransferase 2 antibody
    • Serine palmitoyltransferase long chain base subunit 2 antibody
    • Serine palmitoyltransferase subunit II antibody
    • Serine-palmitoyl-CoA transferase 2 antibody
    • SPT 2 antibody
    • SPT2 antibody
    • SPTC2_HUMAN antibody
    • SPTLC 2 antibody
    • Sptlc2 antibody
    see all

Images

  • All lanes : Anti-Serine Palmitoyltransferase antibody (ab23696) at 1 µg/ml

    Lane 1 : 30ug mouse brain homogenate.
    Lane 2 : 30ug rat kidney supernatant.
    Lane 3 : 30ug human liver microsome.

    Predicted band size: 62 kDa
    Observed band size: 67 kDa
    why is the actual band size different from the predicted?

References

This product has been referenced in:
  • Martínez-Sánchez N  et al. Hypothalamic AMPK-ER Stress-JNK1 Axis Mediates the Central Actions of Thyroid Hormones on Energy Balance. Cell Metab 26:212-229.e12 (2017). WB ; Mouse, Rat . Read more (PubMed: 28683288) »
  • Kawamoto E  et al. Immobilization rapidly induces muscle insulin resistance together with the activation of MAPKs (JNK and p38) and impairment of AS160 phosphorylation. Physiol Rep 4:N/A (2016). Rat . Read more (PubMed: 27482072) »
See all 10 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer, low pH
Specification
lung
Blocking step
Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jul 25 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (whole)
Gel Running Conditions
Reduced Denaturing (4-20%)
Loading amount
50 µg
Treatment
500nM NE
Specification
whole
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jul 20 2017

Answer

Thank you for contacting us.

Here are the testing discount codes as requested:

Anti-IDH3G antibody (ab118289) Discount code: 42379

Anti-Serine Palmitoyltransferase antibody (ab23696): this antibody has already been shown to work with mouse samples as well as IHC of paraffin embedded tissue sections and would therefore be covered by the Abpromise for your customers experiments.

Anti-SLC25A34 antibody (ab82917) Discount code: 41766. As this antibody has not been used for IHC-P or mouse samples, I would therefore suggest that your customer try performing a western blot (tested application)with the antibodybefore trying the IHC-P.

Anti-CLIC4 antibody (ab77626) Discount code: 40682

Anti-ATPase Inhibitory Factor 1 antibody [5E2D7] (ab110277) Discount code: 40831

Anti-UCP2 antibody (ab77363): this antibody has not been tested in mouse or IHC-P. I would therefore suggest using ab116263 as an alternative. This antibody has been used in IHC-P previously and is predicted to work with mouse samples. If you customer would prefer to try this antibody please let me know and I will generate the relevant discount code.

All of the discount codes presented have an expiration date of the 12th of June 2012. If your customer would like more information on the discount scheme this can be found from the following link:

https://www.abcam.com/collaboratordiscount

I hope this information has been of help. I look forward to hearing which antibody your customer would prefer to usefor the UCP2 detection.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse embryonic fibroblast)
Loading amount
50 µg
Specification
Mouse embryonic fibroblast
Gel Running Conditions
Reduced Denaturing (10-20% tris-tricine)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 12 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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