Product nameAnti-Serine Palmitoyltransferase antibody
See all Serine Palmitoyltransferase primary antibodies
DescriptionRabbit polyclonal to Serine Palmitoyltransferase
SpecificityThis antibody detects human serine palmitoyltransferase subunit SPT2
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Sheep, Chicken, Cow, Human, Pig, Xenopus laevis, Zebrafish, African green monkey
- Mouse brain homogenate, rat kidney supernatant, human liver microsome.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.1% BSA, 50% Glycerol, 0.8% Sodium chloride, 0.3% Tris
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis antibody was affinity purifed.
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Our Abpromise guarantee covers the use of ab23696 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 62 kDa.|
|IHC-P||Use a concentration of 1 µg/ml.|
FunctionSerine palmitoyltransferase (SPT). The heterodimer formed with LCB1/SPTLC1 constitutes the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC2-SSSPTA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC2-SSSPTB complex displays a preference for C18-CoA substrate.
Tissue specificityWidely expressed.
PathwayLipid metabolism; sphingolipid metabolism.
Involvement in diseaseDefects in SPTLC2 are the cause of hereditary sensory and autonomic neuropathy type 1C (HSAN1C) [MIM:613640]. It is a form of hereditary sensory and autonomic neuropathy, a genetically and clinically heterogeneous group of disorders characterized by degeneration of dorsal root and autonomic ganglion cells, and by prominent sensory abnormalities with a variable degree of motor and autonomic dysfunction. The neurological phenotype is often complicated by severe infections, osteomyelitis, and amputations. HSAN1C symptoms include loss of touch and vibration in the feet, dysesthesia and severe panmodal sensory loss in the upper and lower limbs, distal lower limb sensory loss with ulceration and osteomyelitis, and distal muscle weakness.
Sequence similaritiesBelongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.
Cellular localizationEndoplasmic reticulum membrane.
- Information by UniProt
- KIAA0526 antibody
- LCB 2 antibody
- LCB2 antibody
All lanes : Anti-Serine Palmitoyltransferase antibody (ab23696) at 1 µg/ml
Lane 1 : 30ug mouse brain homogenate.
Lane 2 : 30ug rat kidney supernatant.
Lane 3 : 30ug human liver microsome.
Predicted band size: 62 kDa
Observed band size: 67 kDa why is the actual band size different from the predicted?
This product has been referenced in:
- Martínez-Sánchez N et al. Hypothalamic AMPK-ER Stress-JNK1 Axis Mediates the Central Actions of Thyroid Hormones on Energy Balance. Cell Metab 26:212-229.e12 (2017). WB ; Mouse, Rat . Read more (PubMed: 28683288) »
- Kawamoto E et al. Immobilization rapidly induces muscle insulin resistance together with the activation of MAPKs (JNK and p38) and impairment of AS160 phosphorylation. Physiol Rep 4:N/A (2016). Rat . Read more (PubMed: 27482072) »