Overview

  • Product name

    Anti-Serine Palmitoyltransferase antibody - C-terminal
    See all Serine Palmitoyltransferase primary antibodies
  • Description

    Mouse polyclonal to Serine Palmitoyltransferase - C-terminal
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Chinese hamster
  • Immunogen

    Recombinant fragment (GST-tag) corresponding to Human Serine Palmitoyltransferase aa 453-561 (C terminal). NP_004854.1.
    Sequence:

    LKEMGFIIYGNEDSPVVPLMLYMPAKIGAFGREMLKRNIGVVVVGFPATP IIESRARFCLSAAHTKEILDTALKEIDEVGDLLQLKYSRHRLVPLLDRPF DETTYEETE


    Database link: O15270

  • Positive control

    • Raw 264.7 and NIH 3T3 cell lysates; Serine Palmitoyltransferase recombinant protein

Properties

Applications

Our Abpromise guarantee covers the use of ab194575 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2500. Predicted molecular weight: 63 kDa.

Target

  • Function

    Serine palmitoyltransferase (SPT). The heterodimer formed with LCB1/SPTLC1 constitutes the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC2-SSSPTA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC2-SSSPTB complex displays a preference for C18-CoA substrate.
  • Tissue specificity

    Widely expressed.
  • Pathway

    Lipid metabolism; sphingolipid metabolism.
  • Involvement in disease

    Defects in SPTLC2 are the cause of hereditary sensory and autonomic neuropathy type 1C (HSAN1C) [MIM:613640]. It is a form of hereditary sensory and autonomic neuropathy, a genetically and clinically heterogeneous group of disorders characterized by degeneration of dorsal root and autonomic ganglion cells, and by prominent sensory abnormalities with a variable degree of motor and autonomic dysfunction. The neurological phenotype is often complicated by severe infections, osteomyelitis, and amputations. HSAN1C symptoms include loss of touch and vibration in the feet, dysesthesia and severe panmodal sensory loss in the upper and lower limbs, distal lower limb sensory loss with ulceration and osteomyelitis, and distal muscle weakness.
  • Sequence similarities

    Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.
  • Cellular localization

    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • KIAA0526 antibody
    • LCB 2 antibody
    • LCB2 antibody
    • LCB2a antibody
    • Long chain base biosynthesis protein 2 antibody
    • Long chain base biosynthesis protein 2a antibody
    • Serine palmitoyl CoA transferase 2 antibody
    • Serine palmitoyltransferase 2 antibody
    • Serine palmitoyltransferase long chain base subunit 2 antibody
    • Serine palmitoyltransferase subunit II antibody
    • Serine-palmitoyl-CoA transferase 2 antibody
    • SPT 2 antibody
    • SPT2 antibody
    • SPTC2_HUMAN antibody
    • SPTLC 2 antibody
    • Sptlc2 antibody
    see all

Images

  • Anti-Serine Palmitoyltransferase antibody - C-terminal (ab194575) at 1/1000 dilution + Serine Palmitoyltransferase recombinant protein (immunizing peptide) at 0.2 µg

    Predicted band size: 63 kDa
    Observed band size: 38.1 kDa
    why is the actual band size different from the predicted?



    The observed band size may not correspond to the predicted protein molecular weight as the immunogen (recombinant fragment) was used for the test lane.

  • Anti-Serine Palmitoyltransferase antibody - C-terminal (ab194575) at 1/500 dilution + Raw 264.7 cell lysate at 50 µg

    Predicted band size: 63 kDa

  • Anti-Serine Palmitoyltransferase antibody - C-terminal (ab194575) at 1/500 dilution + NIH 3T3 cell lysate at 50 µg

    Predicted band size: 63 kDa

References

ab194575 has not yet been referenced specifically in any publications.

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