Anti-Serine racemase antibody (ab224620)
- Datasheet
- References
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Overview
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Product name
Anti-Serine racemase antibody
See all Serine racemase primary antibodies -
Description
Rabbit polyclonal to Serine racemase -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant full length protein corresponding to Human Serine racemase aa 1-340.
Sequence:MCAQYCISFADVEKAHINIRDSIHLTPVLTSSILNQLTGRNLFFKCELFQ KTGSFKIRGALNAVRSLVPDALERKPKAVVTHSSGNHGQALTYAAKLEGI PAYIVVPQTAPDCKKLAIQAYGASIVYCEPSDESRENVAKRVTEETEGIM VHPNQEPAVIAGQGTIALEVLNQVPLVDALVVPVGGGGMLAGIAITVKAL KPSVKVYAAEPSNADDCYQSKLKGKLMPNLYPPETIADGVKSSIGLNTWP IIRDLVDDIFTVTEDEIKCATQLVWERMKLLIEPTAGVGVAAVLSQHFQT VSPEVKNICIVLSGGNVDLTSSITWVKQAERPASYQSVSV
Database link: Q9GZT4 -
Positive control
- WB: Mouse heart and brain tissue lysates. IHC-P: Human heart and brain tissues.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin
Constituents: 50% Glycerol, PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab224620 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/5000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa). | |
| IHC-P | 1/20 - 1/200. |
Target
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Function
Catalyzes the synthesis of D-serine from L-serine. D-serine is a key coagonist with glutamate at NMDA receptors. Has dehydratase activity towards both L-serine and D-serine. -
Tissue specificity
Brain: expressed at high levels in hippocampus and corpus callosum, intermediate levels in substantia nigra and caudate, and low levels in amygdala, thalamus, and subthalamic nuclei. Expressed in heart, skeletal muscle, kidney and liver. -
Sequence similarities
Belongs to the serine/threonine dehydratase family. -
Post-translational
modificationsS-nitrosylated, leading to decrease the enzyme activity. - Information by UniProt
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Database links
- Entrez Gene: 63826 Human
- Entrez Gene: 27364 Mouse
- Omim: 606477 Human
- SwissProt: Q9GZT4 Human
- SwissProt: Q9QZX7 Mouse
- Unigene: 461954 Human
- Unigene: 131443 Mouse
- Unigene: 220843 Mouse
see all -
Alternative names
- D serine ammonia lyase antibody
- D serine dehydratase antibody
- D-serine ammonia-lyase antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serine racemase antibody (ab224620)Paraffin-embedded human heart tissue stained for Serine racemase using ab224620 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serine racemase antibody (ab224620)Paraffin-embedded human brain tissue stained for Serine racemase using ab224620 at 1/100 dilution in immunohistochemical analysis.
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Western blot - Anti-Serine racemase antibody (ab224620)All lanes : Anti-Serine racemase antibody (ab224620) at 1/500 dilution
Lane 1 : Mouse heart tissue lysate
Lane 2 : Mouse brain tissue lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 37 kDa
Observed band size: 37 kDa
References
ab224620 has not yet been referenced specifically in any publications.
