Recombinant Anti-Serine racemase antibody [EPR16682] (ab200833)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16682] to Serine racemase
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Serine racemase antibody [EPR16682]
See all Serine racemase primary antibodies -
Description
Rabbit monoclonal [EPR16682] to Serine racemase -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: LNCaP, U-87 MG and HEK293 whole cell lysates; Human fetal brain lysate. IP: HEK293 and LNCaP whole cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16682 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab200833 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
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IP |
1/50.
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Notes |
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WB
1/2000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa). |
IP
1/50. |
Target
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Function
Catalyzes the synthesis of D-serine from L-serine. D-serine is a key coagonist with glutamate at NMDA receptors. Has dehydratase activity towards both L-serine and D-serine. -
Tissue specificity
Brain: expressed at high levels in hippocampus and corpus callosum, intermediate levels in substantia nigra and caudate, and low levels in amygdala, thalamus, and subthalamic nuclei. Expressed in heart, skeletal muscle, kidney and liver. -
Sequence similarities
Belongs to the serine/threonine dehydratase family. -
Post-translational
modificationsS-nitrosylated, leading to decrease the enzyme activity. - Information by UniProt
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Database links
- Entrez Gene: 63826 Human
- Omim: 606477 Human
- SwissProt: Q9GZT4 Human
- Unigene: 461954 Human
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Alternative names
- D serine ammonia lyase antibody
- D serine dehydratase antibody
- D-serine ammonia-lyase antibody
see all
Images
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All lanes : Anti-Serine racemase antibody [EPR16682] (ab200833) at 1/5000 dilution
Lane 1 : LNCaP (Human prostate cancer cell line) whole cell lysate
Lane 2 : Human fetal brain tissue lysate
Lane 3 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Lane 4 : HEK-293 (Human embryonic kidney) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 37 kDa
Observed band size: 37 kDa
Exposure time: 3 minutesBlocking/Dilution Buffer: 5% NFDM/TBST.
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Serine racemase was immunoprecipitated from 1mg of HEK293 (Human embryonic kidney) whole cell lysate with ab200833 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab200833 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: HEK293 whole cell lysate 10 µg (Input). Lane 2: ab200833 IP in HEK293 whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200833 in HEK293 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.Exposure time: 30 seconds
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Serine racemase was immunoprecipitated from 1mg of LNCaP (Human prostate cancer cell line) whole cell lysate with ab200833 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab200833 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: LNCaP whole cell lysate 10 µg (Input). Lane 2: ab200833 IP in LNCaP whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200833 in LNCaP whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.Exposure time: 10 seconds
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab200833 has been referenced in 1 publication.
- Ohshima K et al. Serine racemase enhances growth of colorectal cancer by producing pyruvate from serine. Nat Metab 2:81-96 (2020). PubMed: 32694681