Overview

  • Product name

    Anti-Serine racemase antibody [EPR16682]
    See all Serine racemase primary antibodies
  • Description

    Rabbit monoclonal [EPR16682] to Serine racemase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: ICC/IF
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Serine racemase aa 50-150. The exact sequence is proprietary.
    Database link: Q9GZT4

  • Positive control

    • WB: LNCaP, U-87 MG and HEK293 whole cell lysates; Human fetal brain lysate. IP: HEK293 and LNCaP whole cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab200833 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
IP 1/50.
  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function

      Catalyzes the synthesis of D-serine from L-serine. D-serine is a key coagonist with glutamate at NMDA receptors. Has dehydratase activity towards both L-serine and D-serine.
    • Tissue specificity

      Brain: expressed at high levels in hippocampus and corpus callosum, intermediate levels in substantia nigra and caudate, and low levels in amygdala, thalamus, and subthalamic nuclei. Expressed in heart, skeletal muscle, kidney and liver.
    • Sequence similarities

      Belongs to the serine/threonine dehydratase family.
    • Post-translational
      modifications

      S-nitrosylated, leading to decrease the enzyme activity.
    • Information by UniProt
    • Database links

    • Alternative names

      • D serine ammonia lyase antibody
      • D serine dehydratase antibody
      • D-serine ammonia-lyase antibody
      • D-serine dehydratase antibody
      • ILV1 antibody
      • ISO1 antibody
      • L serine ammonia lyase antibody
      • L serine dehydratase antibody
      • L-serine ammonia-lyase antibody
      • L-serine dehydratase antibody
      • Serine racemase antibody
      • srr antibody
      • SRR_HUMAN antibody
      see all

    Images

    • All lanes : Anti-Serine racemase antibody [EPR16682] (ab200833) at 1/5000 dilution

      Lane 1 : LNCaP (Human prostate cancer cell line) whole cell lysate
      Lane 2 : Human fetal brain lysate
      Lane 3 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
      Lane 4 : HEK293 (Human embryonic kidney) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated

      Predicted band size: 37 kDa
      Observed band size: 37 kDa


      Exposure time: 3 minutes


      Blocking/Dilution Buffer: 5% NFDM/TBST.

    • Serine racemase was immunoprecipitated from 1mg of HEK293 (Human embryonic kidney) whole cell lysate with ab200833 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab200833 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

      Lane 1: HEK293 whole cell lysate 10 µg (Input). Lane 2: ab200833 IP in HEK293 whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200833 in HEK293 whole cell lysate.
      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 30 seconds

    • Serine racemase was immunoprecipitated from 1mg of LNCaP (Human prostate cancer cell line) whole cell lysate with ab200833 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab200833 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

      Lane 1: LNCaP whole cell lysate 10 µg (Input). Lane 2: ab200833 IP in LNCaP whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200833 in LNCaP whole cell lysate.
      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 10 seconds

    References

    ab200833 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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