Product nameAnti-Serine racemase antibody [EPR18747]
See all Serine racemase primary antibodies
DescriptionRabbit monoclonal [EPR18747] to Serine racemase
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Rat
Recombinant fragment within Mouse Serine racemase aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: Q9QZX7
- WB: Mouse and rat brain and liver tissue lysate. IP: Mouse brain tissue lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab182217 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 34-36 kDa (predicted molecular weight: 37 kDa).|
FunctionCatalyzes the synthesis of D-serine from L-serine. D-serine is a key coagonist with glutamate at NMDA receptors. Has dehydratase activity towards both L-serine and D-serine.
Tissue specificityBrain: expressed at high levels in hippocampus and corpus callosum, intermediate levels in substantia nigra and caudate, and low levels in amygdala, thalamus, and subthalamic nuclei. Expressed in heart, skeletal muscle, kidney and liver.
Sequence similaritiesBelongs to the serine/threonine dehydratase family.
modificationsS-nitrosylated, leading to decrease the enzyme activity.
- Information by UniProt
- D serine ammonia lyase antibody
- D serine dehydratase antibody
- D-serine ammonia-lyase antibody
All lanes : Anti-Serine racemase antibody [EPR18747] (ab182217) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse heart tissue lysate
Lane 3 : Mouse liver tissue lysate
Lane 4 : Rat brain tissue lysate
Lane 5 : Rat heart tissue lysate
Lane 6 : Rat liver tissue lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 37 kDa
Observed band size: 34-36 kDa why is the actual band size different from the predicted?
Exposure time: 41 seconds
Blocking/Dilution buffer and concentration: 5% NFDM/TBST
The expression profile observed is consistent with the literature; heart tissue is expected to be negative for serine racemase (PMID: 10557334).
Serine racemase was immunoprecipitated from 0.35 mg of mouse brain tissue lysate with ab182217 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab182217 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1000 dilution.
Lane 1: Mouse brain tissue lysate 10 µg (Input).
Lane 2: ab182217 IP in mouse brain tissue lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab182217 in mouse brain tissue lysate.
Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
ab182217 has not yet been referenced specifically in any publications.