Overview

  • Product name

    Anti-Serine racemase antibody [EPR18747]
    See all Serine racemase primary antibodies
  • Description

    Rabbit monoclonal [EPR18747] to Serine racemase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Recombinant fragment within Mouse Serine racemase aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9QZX7

  • Positive control

    • WB: Mouse and rat brain and liver tissue lysate. IP: Mouse brain tissue lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab182217 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 34-36 kDa (predicted molecular weight: 37 kDa).
IP 1/30.

Target

  • Function

    Catalyzes the synthesis of D-serine from L-serine. D-serine is a key coagonist with glutamate at NMDA receptors. Has dehydratase activity towards both L-serine and D-serine.
  • Tissue specificity

    Brain: expressed at high levels in hippocampus and corpus callosum, intermediate levels in substantia nigra and caudate, and low levels in amygdala, thalamus, and subthalamic nuclei. Expressed in heart, skeletal muscle, kidney and liver.
  • Sequence similarities

    Belongs to the serine/threonine dehydratase family.
  • Post-translational
    modifications

    S-nitrosylated, leading to decrease the enzyme activity.
  • Information by UniProt
  • Database links

  • Alternative names

    • D serine ammonia lyase antibody
    • D serine dehydratase antibody
    • D-serine ammonia-lyase antibody
    • D-serine dehydratase antibody
    • ILV1 antibody
    • ISO1 antibody
    • L serine ammonia lyase antibody
    • L serine dehydratase antibody
    • L-serine ammonia-lyase antibody
    • L-serine dehydratase antibody
    • Serine racemase antibody
    • srr antibody
    • SRR_HUMAN antibody
    see all

Images

  • All lanes : Anti-Serine racemase antibody [EPR18747] (ab182217) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse heart tissue lysate
    Lane 3 : Mouse liver tissue lysate
    Lane 4 : Rat brain tissue lysate
    Lane 5 : Rat heart tissue lysate
    Lane 6 : Rat liver tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 37 kDa
    Observed band size: 34-36 kDa
    why is the actual band size different from the predicted?


    Exposure time: 41 seconds


    Blocking/Dilution buffer and concentration: 5% NFDM/TBST

    The expression profile observed is consistent with the literature; heart tissue is expected to be negative for serine racemase (PMID: 10557334).

  • Serine racemase was immunoprecipitated from 0.35 mg of mouse brain tissue lysate with ab182217 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab182217 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1000 dilution.

    Lane 1: Mouse brain tissue lysate 10 µg (Input). 

    Lane 2: ab182217 IP in mouse brain tissue lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab182217 in mouse brain tissue lysate.

    Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

References

ab182217 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Cortical Brain Tissue)
Antigen retrieval step
None
Permeabilization
No
Specification
Cortical Brain Tissue
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Feb 06 2019

Application
Western blot
Sample
Mouse Tissue lysate - whole (Cortical Brain Tissue)
Gel Running Conditions
Reduced Denaturing (4-20%)
Loading amount
20 µg
Specification
Cortical Brain Tissue
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Feb 06 2019

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