Product nameAnti-Serine/threonine-protein kinase 4/MST-1 antibody
See all Serine/threonine-protein kinase 4/MST-1 primary antibodies
DescriptionRabbit polyclonal to Serine/threonine-protein kinase 4/MST-1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Monkey
Synthetic peptide within Human MST1 aa 375-425. The exact sequence is proprietary.
Database link: Q13043
- WB: HeLa, Ramos and NIH/3T3 whole cell lysate. IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264127 in the following tested applications.
|WB||1/500 - 1/2500. Predicted molecular weight: 56 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionStress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. MST1/MST2 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation (By similarity). Phosphorylates 'Ser-14' of histone H2B (H2BS14ph) during apoptosis. Phosphorylates FOXO3 upon oxidative stress, which results in its nuclear translocation and cell death initiation.
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 protein kinase domain.
Contains 1 SARAH domain.
modificationsAutophosphorylated on serine and threonine residues.
Cellular localizationCytoplasm. Nucleus. The caspase-cleaved form cycles between the nucleus and cytoplasm.
- Information by UniProt
- Kinase responsive to stress antibody
- Krs2 antibody
- Mammalian STE20 like protein kinase 1 antibody
All lanes : Anti-Serine/threonine-protein kinase 4/MST-1 antibody (ab264127) at 1 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : Ramos (Human Burkitt's lymphoma cell line) whole cell lysate at 50 µg
Lane 5 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Predicted band size: 56 kDa
Exposure time: 30 seconds
Whole cell lysate (1 mg/IP reaction; 1/4 of reaction loaded/gel) from HeLa cells. ab264127 used at 3 μg/mg lysate for IP. MST1 and MST2 were simultaneously immunoprecipitated using MST1/2 antibodies.
Lane 1: ab264127.
Lane 2: MST1-2/STK3-4 antibody.
Lane 3: MST2/STK3 antibody.
Lane 4: Another MST1-2/STK3-4 antibody.
Lane 5: Control IgG.
Lane 6: Input.
ab264127 is specific for Serine/threonine-protein kinase 4/MST-1.
ab264127 has not yet been referenced specifically in any publications.