Product nameAnti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y]
DescriptionRabbit monoclonal [EP1465Y] to Serine/threonine-protein kinase 4/MST-1
Tested applicationsSuitable for: Flow Cyt, WB, IHC-P, IHC-Fr, ICC/IF, IPmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Serine/threonine-protein kinase 4/MST-1 aa 1-100 (N terminal). The exact sequence is proprietary.
- WB: Jurkat and HeLa cell lysate and mouse spleen, rat spleen and human urinary bladder tissues. ICC/IF: Raw264.7 cells. IHC-P:Human gastric carcinoma. IP: Jurkat cell lysate. Flow Cyt: HeLa cells.
Previously labelled as Serine/threonine-protein kinase 4
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.21% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab51134 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000. Detects a band of approximately 59 kDa (predicted molecular weight: 56 kDa).|
|IHC-P||1/50 - 1/250.
We strongly recommend that customers perform an antigen retrieval step.
|IHC-Fr||Use at an assay dependent concentration. PubMed: 24595170|
|ICC/IF||1/100 - 1/250.|
|IP||1/30 - 1/100.|
FunctionStress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. MST1/MST2 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation (By similarity). Phosphorylates 'Ser-14' of histone H2B (H2BS14ph) during apoptosis. Phosphorylates FOXO3 upon oxidative stress, which results in its nuclear translocation and cell death initiation.
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 protein kinase domain.
Contains 1 SARAH domain.
modificationsAutophosphorylated on serine and threonine residues.
Cellular localizationCytoplasm. Nucleus. The caspase-cleaved form cycles between the nucleus and cytoplasm.
- Information by UniProt
- Kinase responsive to stress antibody
- Krs2 antibody
- Mammalian STE20 like protein kinase 1 antibody
All lanes : Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] (ab51134) at 1/50000 dilution
Lane 1 : Jurkat (human acute T cell leukemia) whole cell lysate
Lane 2 : Mouse spleen
Lane 3 : Rat spleen
Lysates/proteins at 1/20 dilution per lane.
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 56 kDa
Diluting and blocking buffer: 5% NFDM/TBST
ab51134 staining Serine/threonine-protein kinase 4/MST-1 in Raw264.7 (mouse abelson murine leukemia virus-induced tumor) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a concentration of 1/1000. ab7291 anti-Tubulin (mouse mAb) (1/1000) and ab150120 AlexaFluor®594 Goat anti-Mouse secondary (1/1000) were used as counterstains for primary antibody ab51134 and secondary antibody ab150077 respectively and DAPI was used as a nuclear counterstain.
ab51134 staining Serine/threonine-protein kinase 4/MST-1 in human gastric carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/50. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.
Negative control 1: PBS in place of primary antibody.
Flow Cytometry analysis of HeLa cells labelling Serine/theronine-protein kinase 4/MST-1 with purified ab51134 at a dilution of 1/50 (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. An Alexa Flour® 488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
ab51134 immunoprecipitating Serine/threonine-protein kinase 4/MST-1. 10µg of cell lysate was incubated with primary antibody at a dilution of 1/30 and VeriBlot for IP Detection Reagent (HRP) (ab131366) at a dilution of 1/1000.
Lane 1: Jurkat (human acute T cell leukemia) whole cell lysate (10ug)
Lane 2: Jurkat (human acute T cell leukemia) whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab51134 in Jurkat (human acute T cell leukemia) whole cell lysate
Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] (ab51134) at 1/10000 dilution + HeLa (human cervix adenocarcinoma) whole cell lysate at 20 µg
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 56 kDa
Diluting and blocking buffer: 5% NFDM/TBST
This product has been referenced in:
- Hu L et al. Tri-ortho-cresyl phosphate (TOCP) induced ovarian failure in mice is related to the Hippo signaling pathway disruption. Reprod Toxicol 83:21-27 (2019). Read more (PubMed: 30439503) »
- Cheng Z et al. Cardiac-specific Mst1 deficiency inhibits ROS-mediated JNK signalling to alleviate Ang II-induced cardiomyocyte apoptosis. J Cell Mol Med 23:543-555 (2019). Read more (PubMed: 30338935) »