RecombinantRabMAb

Recombinant Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] - BSA and Azide free (ab232551)

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Flow Cytometry - Anti-Serine/threonine-protein kinase 4 antibody [EP1465Y] - BSA and Azide free (ab232551)
  • Immunoprecipitation - Anti-Serine/threonine-protein kinase 4 antibody [EP1465Y] - BSA and Azide free (ab232551)
  • Immunocytochemistry/ Immunofluorescence - Anti-Serine/threonine-protein kinase 4 antibody [EP1465Y] - BSA and Azide free (ab232551)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serine/threonine-protein kinase 4 antibody [EP1465Y] - BSA and Azide free (ab232551)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1465Y] to Serine/threonine-protein kinase 4/MST-1 - BSA and Azide free
  • Suitable for: Flow Cyt, IP, IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] - BSA and Azide free

  • Description

    Rabbit monoclonal [EP1465Y] to Serine/threonine-protein kinase 4/MST-1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, IP, IHC-P, WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Serine/threonine-protein kinase 4 aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • IHC-P: Human gastric carcinoma tissue.

  • General notes

    ab232551 is the carrier-free version of ab51134. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab232551 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EP1465Y

  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab232551 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

We strongly recommend that customers perform an antigen retrieval step.
WB Use at an assay dependent concentration. Detects a band of approximately 59 kDa (predicted molecular weight: 55 kDa).
ICC/IF Use at an assay dependent concentration.

Images

  • Flow Cytometry - Anti-Serine/threonine-protein kinase 4 antibody [EP1465Y] - BSA and Azide free (ab232551)
    Flow Cytometry - Anti-Serine/threonine-protein kinase 4 antibody [EP1465Y] - BSA and Azide free (ab232551)

    Flow Cytometry analysis of HeLa cells labelling Serine/theronine-protein kinase 4 /MST-1 with purified ab51134 at a dilution of 1/50 (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. An Alexa Flour® 488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51134).

  • Immunoprecipitation - Anti-Serine/threonine-protein kinase 4 antibody [EP1465Y] - BSA and Azide free (ab232551)
    Immunoprecipitation - Anti-Serine/threonine-protein kinase 4 antibody [EP1465Y] - BSA and Azide free (ab232551)

    ab51134 immunoprecipitating Serine/threonine-protein kinase 4. 10µg of cell lysate was incubated with primary antibody at a dilution of 1/30 and VeriBlot for IP Detection Reagent (HRP) (ab131366) at a dilution of 1/1000.

    Lane 1: Jurkat (human acute T cell leukemia) whole cell lysate (10ug)
    Lane 2: Jurkat (human acute T cell leukemia) whole cell lysate
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab51134 in Jurkat (human acute T cell leukemia) whole cell lysate

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51134).

  • Immunocytochemistry/ Immunofluorescence - Anti-Serine/threonine-protein kinase 4 antibody [EP1465Y] - BSA and Azide free (ab232551)
    Immunocytochemistry/ Immunofluorescence - Anti-Serine/threonine-protein kinase 4 antibody [EP1465Y] - BSA and Azide free (ab232551)

    ab51134 staining Serine/threonine-protein kinase 4 / MST-1 in Raw264.7 (mouse abelson murine leukemia virus-induced tumor) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a concentration of 1/1000. ab7291 anti-Tubulin (mouse mAb) (1/1000) and ab150120 AlexaFluor®594 Goat anti-Mouse secondary (1/1000)  were used as counterstains for primary antibody ab51134 and secondary antibody ab150077 respectively and DAPI was used as a nuclear counterstain.

    Negative control 1: Rabbit primary antibody and anti-mouse secondary antibody (ab150120)
    Negative control 2: Mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51134).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serine/threonine-protein kinase 4 antibody [EP1465Y] - BSA and Azide free (ab232551)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serine/threonine-protein kinase 4 antibody [EP1465Y] - BSA and Azide free (ab232551)

    ab51134 staining Serine/threonine-protein kinase 4 in human gastric carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/50. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

    Negative control 1: PBS in place of primary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51134).

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (1)

Publishing research using ab232551? Please let us know so that we can cite the reference in this datasheet.

ab232551 has been referenced in 1 publication.

  • Wu X  et al. RNA Binding Protein RNPC1 Suppresses the Stemness of Human Endometrial Cancer Cells via Stabilizing MST1/2 mRNA. Med Sci Monit 26:e921389 (2020). PubMed: 32088727

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