Anti-Serotonin antibody [YC5/45] (ab6336)

Reviews (1)Q&A (6)References (13)

Overview

  • Product name

    Anti-Serotonin antibody [YC5/45]
    See all Serotonin primary antibodies

  • Description

    Rat monoclonal [YC5/45] to Serotonin

  • Host species

    Rat

  • Tested applications

    Suitable for: IHC-P, IHC-Fr, ICC/IF, IHC-FoFrmore details

  • Species reactivity

    Reacts with: Species independent

  • Immunogen

    5HT-bovine seroalbumin.

  • Positive control

    • In IHC, this antibody gave a positive signal in a formalin-fixed, paraffin-embedded coronal section of Mouse Brain.

  • General notes


    Useful for the diagnosis of carcinoid tumours and the investigation of the effects of drugs on the neurological system.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Primary antibody notes

    Useful for the diagnosis of carcinoid tumours and the investigation of the effects of drugs on the neurological system.

  • Clonality

    Monoclonal

  • Clone number

    YC5/45

  • Isotype

    IgG2c

  • Light chain type

    kappa

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6336 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. PubMed: 22474435
IHC-Fr Use a concentration of 1 - 5 µg/ml. (see Ni et al). Fix with ice cold acetone. Dilute antibody in 1.5% blocking serum.
ICC/IF Use at an assay dependent concentration.
IHC-FoFr 1/100 - 1/1000. Used on gelatin embedded perfusion fixed tissue (see recommended protocol).

Target

  • Relevance

    Serotonin (5-hydroxytryptamine, or 5-HT) is a monoamine neurotransmitter synthesised in serotonergic neurons in the central nervous system and enterochromaffin cells in the gastrointestinal tract. Serotonin plays an important part in the biochemistry of depression, migraine, bipolar disorder and anxiety. It is also believed to be influential on sexuality and appetite. 5-HT is generally thought not to be released from synaptic terminal buttons in the manner of classical neurotransmission but from serotonergic varacosities into the extra neuronal space. From here it is free to diffuse over a relatively large region of space (>20µm) and activate 5-HT receptors located on the dendrites, cell bodies and presynaptic terminals of adjacent neurons. Serotonergic action is terminated primarily via uptake of 5-HT from the synapse. This is through the specific monoamine transporter for 5-HT, 5-HT reuptake transporter, on the presynaptic neuron. The pharmacology of 5-HT is extremely complex, with its actions being mediated by a large and diverse range of 5-HT receptors.

  • Cellular localization

    Cytoplasmic and Secreted

  • Alternative names

    • 5 hydroxytryptamine antibody
    • 5HT antibody

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serotonin antibody [YC5/45] (ab6336)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serotonin antibody [YC5/45] (ab6336)

    ab6336 in rat brain tissue fixed with 4% paraformaldehyde. The staining is appropriate, lighting up cell bodies, dendrites and terminals of known 5-HT containing neurones. These pictures are from the review provided by Jim Deuchars.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serotonin antibody [YC5/45] (ab6336)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serotonin antibody [YC5/45] (ab6336)
    IHC image of Serotonin staining in mouse brain (coronal) formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab6336, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • Immunohistochemistry (Frozen sections) - Anti-Serotonin antibody [YC5/45] (ab6336)
    Immunohistochemistry (Frozen sections) - Anti-Serotonin antibody [YC5/45] (ab6336)This image is courtesy of Ms. Szu-yu Liu by an abreview

    Paraformaldehyde-fixed rabbit spinal cord tissue stained for Serotonin using ab6336 at 1/200 dilution in immunohistochemical analysis. Power Block™ Universal Blocking Reagen t(Biogenex) was used as ablocking agent for 10 minute(s) at a concentration of 10% at 37°C. Biotin-SP-conjugated Goat Anti-Rat IgG was used as the secondary antibody at 1/500 dilution.

    See Abreview

References

This product has been referenced in:

  • Zhang W  et al. Wheel Running Improves Motor Function and Spinal Cord Plasticity in Mice With Genetic Absence of the Corticospinal Tract. Front Cell Neurosci 13:106 (2019). Read more (PubMed: 30941019) »
  • Humenick A  et al. Characterization of projections of longitudinal muscle motor neurons in human colon. Neurogastroenterol Motil 31:e13685 (2019). Read more (PubMed: 31355986) »
See all 13 Publications for this product

Publishing research using ab6336? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Immunohistochemistry (Frozen sections) abreview for Anti-Serotonin antibody [YC5/45]

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Rabbit Tissue sections (spinal cord)
Specification
spinal cord
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Power Block™ Universal Blocking Reagent(Biogenex) as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 37°C
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Ms. Szu-yu Liu

Verified customer

Submitted Nov 23 2012

Question

Product code: 6336
Inquiry: Dear abcam technical support team: This customer would like to order ab6336 (Anti-Serotonin antibody [YC5/45]) antibody) and test in IHC-Fr with rabbit sample, would you please help this customer to create a discount code, the information of this customer as follow: xxxxxxx
Could you please help this customer to solve this problem? Thanks for your kindly help. Best regards

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Answer

Thank you for contacting us.

The anitbody ab6336 has been tested in IHC-Fr but as yet has not been used with rabbit samples. This is therefore information that we would be very interested in. I have therefore generated the discount code as requested which is stated below:

Discount code: xxxxxxx

Expiration date: 24th October 2012

If you have any further questions please do not hesitate to ask.

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Question

I'm interested in your rat anti-serotonin monoclonal antibody (YC5/45); code ab6336. However It is in the culture supernant form. Could you tell me what is the concentration of the antibody? Thanks! Have a nice holiday!

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Answer

Thank you for your enquiry. Ab6336 is in an unpurified form, tissue culture supernatant, and we cannnot guarantee the concentration for unpurified material.

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Question

Do you have antibody to serotonin transporter for cats?

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Answer

Thank you for your enquiry. I did a search in our online catalog and the following antibodies are the serotonin antibodies which we currently have available: ab3439, ab3440, ab6444, ab3505, ab3506, ab6336, ab8882, ab2403, and ab949. Unfortunately, none of them have yet been tested for cross-reactivity in cats. Should you decide to go ahead and purchase any of these products, please let us know how you get on and in return we will forward a reward of your choice, typically an Amazon gift voucher. If you have any more questions, please contact us again.

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Question

Antibody requested: 5HT2B, 5HT2C, 5HT1B (1) are these clones of Serotonin? (2) what company has them available?

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Answer

We produce some of our antibodies in-house and others are sourced from suppliers whose products we believe will be of interest to our customers. The identity of our suppliers remains commercially sensitive information.

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Question

I have tested this antibody on formaldehyde fixed Xenopus laevis whole embryos (stages 28 to 34) and I have not detected a reliable signal. I used concentrations of 1:1, 1:10 and 1:20. Do you have any information on the use of this antibody with Xenopus samples?

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Answer

The serotonin antibody was tested on mature frog tissue.

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Question

I would like to lable serotinergic cells in chicken brain on cryosections (perhaps Paraffin) with fluorescence. Can you recomand this antibody for this purpose?

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Answer

So far, this antibody has not been tested on chicken samples.

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