Anti-Serotonin antibody [YC5/45] (ab6336)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serotonin antibody [YC5/45] (ab6336)](https://www.abcam.com/ps/products/6/ab6336/Images/ab6336_4.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serotonin antibody [YC5/45] (ab6336)")
Key features and details
- Rat monoclonal [YC5/45] to Serotonin
- Suitable for: IHC-Fr, IHC-P, ICC/IF, IHC-FoFr
- Reacts with: Species independent
- Isotype: IgG2c
Overview
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Product name
Anti-Serotonin antibody [YC5/45]
See all Serotonin primary antibodies -
Description
Rat monoclonal [YC5/45] to Serotonin -
Host species
Rat -
Tested applications
Suitable for: IHC-Fr, IHC-P, ICC/IF, IHC-FoFrmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
5HT-bovine seroalbumin.
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Positive control
- In IHC, this antibody gave a positive signal in a formalin-fixed, paraffin-embedded coronal section of Mouse Brain.
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General notes
Useful for the diagnosis of carcinoid tumours and the investigation of the effects of drugs on the neurological system.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Tissue culture supernatant -
Primary antibody notes
Useful for the diagnosis of carcinoid tumours and the investigation of the effects of drugs on the neurological system. -
Clonality
Monoclonal -
Clone number
YC5/45 -
Isotype
IgG2c -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6336 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | (1) |
Use a concentration of 1 - 5 µg/ml. (see Ni et al). Fix with ice cold acetone. Dilute antibody in 1.5% blocking serum.
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| IHC-P | (1) |
Use at an assay dependent concentration.
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| ICC/IF |
Use at an assay dependent concentration.
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| IHC-FoFr |
1/100 - 1/1000. Used on gelatin embedded perfusion fixed tissue (see recommended protocol).
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| Notes |
|---|
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IHC-Fr
Use a concentration of 1 - 5 µg/ml. (see Ni et al). Fix with ice cold acetone. Dilute antibody in 1.5% blocking serum. |
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IHC-P
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. |
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IHC-FoFr
1/100 - 1/1000. Used on gelatin embedded perfusion fixed tissue (see recommended protocol). |
Target
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Relevance
Serotonin (5-hydroxytryptamine, or 5-HT) is a monoamine neurotransmitter synthesised in serotonergic neurons in the central nervous system and enterochromaffin cells in the gastrointestinal tract. Serotonin plays an important part in the biochemistry of depression, migraine, bipolar disorder and anxiety. It is also believed to be influential on sexuality and appetite. 5-HT is generally thought not to be released from synaptic terminal buttons in the manner of classical neurotransmission but from serotonergic varacosities into the extra neuronal space. From here it is free to diffuse over a relatively large region of space (>20µm) and activate 5-HT receptors located on the dendrites, cell bodies and presynaptic terminals of adjacent neurons. Serotonergic action is terminated primarily via uptake of 5-HT from the synapse. This is through the specific monoamine transporter for 5-HT, 5-HT reuptake transporter, on the presynaptic neuron. The pharmacology of 5-HT is extremely complex, with its actions being mediated by a large and diverse range of 5-HT receptors. -
Cellular localization
Cytoplasmic and Secreted -
Alternative names
- 5 hydroxytryptamine antibody
- 5HT antibody
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serotonin antibody [YC5/45] (ab6336)
ab6336 in rat brain tissue fixed with 4% paraformaldehyde. The staining is appropriate, lighting up cell bodies, dendrites and terminals of known 5-HT containing neurones. These pictures are from the review provided by Jim Deuchars.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serotonin antibody [YC5/45] (ab6336)](https://www.abcam.com/ps/products/6/ab6336/Images/ab6336-12285-anti-serotonin-antibody-yc5-45-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serotonin antibody [YC5/45] (ab6336)IHC image of Serotonin staining in mouse brain (coronal) formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab6336, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
![Immunohistochemistry (Frozen sections) - Anti-Serotonin antibody [YC5/45] (ab6336)](https://www.abcam.com/ps/products/6/ab6336/Images/ab6336-305083-anti-serotonin-antibody-yc5-45-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-Serotonin antibody [YC5/45] (ab6336)This image is courtesy of Ms. Szu-yu Liu by an abreviewParaformaldehyde-fixed rabbit spinal cord tissue stained for Serotonin using ab6336 at 1/200 dilution in immunohistochemical analysis. Power Block™ Universal Blocking Reagen t(Biogenex) was used as ablocking agent for 10 minute(s) at a concentration of 10% at 37°C. Biotin-SP-conjugated Goat Anti-Rat IgG was used as the secondary antibody at 1/500 dilution.
Datasheets and documents
References (15)
ab6336 has been referenced in 15 publications.
- Zhang W et al. Wheel Running Improves Motor Function and Spinal Cord Plasticity in Mice With Genetic Absence of the Corticospinal Tract. Front Cell Neurosci 13:106 (2019). PubMed: 30941019
- Humenick A et al. Characterization of projections of longitudinal muscle motor neurons in human colon. Neurogastroenterol Motil 31:e13685 (2019). PubMed: 31355986
- König D et al. Distribution and Restoration of Serotonin-Immunoreactive Paraneuronal Cells During Caudal Fin Regeneration in Zebrafish. Front Mol Neurosci 12:227 (2019). PubMed: 31616250
- Sitaraman D et al. Discrete Serotonin Systems Mediate Memory Enhancement and Escape Latencies after Unpredicted Aversive Experience inDrosophilaPlace Memory. Front Syst Neurosci 11:92 (2017). PubMed: 29321732
- Dhillon RS et al. Axonal plasticity underpins the functional recovery following surgical decompression in a rat model of cervical spondylotic myelopathy. Acta Neuropathol Commun 4:89 (2016). IHC-Fr . PubMed: 27552807
