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    serotonin-antibody-yc545-ab6336.pdf

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Neuroscience Neurotransmitter Biogenic Amines Serotonin / 5HT
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Anti-Serotonin antibody [YC5/45] (ab6336)

  • Datasheet
Reviews (2)Q&A (6)References (15)

Product price, shipping and contact information

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serotonin antibody [YC5/45] (ab6336)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serotonin antibody [YC5/45] (ab6336)
  • Immunohistochemistry (Frozen sections) - Anti-Serotonin antibody [YC5/45] (ab6336)

Key features and details

  • Rat monoclonal [YC5/45] to Serotonin
  • Suitable for: IHC-Fr, IHC-P, ICC/IF, IHC-FoFr
  • Reacts with: Species independent
  • Isotype: IgG2c

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Overview

  • Product name

    Anti-Serotonin antibody [YC5/45]
    See all Serotonin primary antibodies
  • Description

    Rat monoclonal [YC5/45] to Serotonin
  • Host species

    Rat
  • Tested applications

    Suitable for: IHC-Fr, IHC-P, ICC/IF, IHC-FoFrmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    5HT-bovine seroalbumin.

  • Positive control

    • In IHC, this antibody gave a positive signal in a formalin-fixed, paraffin-embedded coronal section of Mouse Brain.
  • General notes


    Useful for the diagnosis of carcinoid tumours and the investigation of the effects of drugs on the neurological system.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Primary antibody notes

    Useful for the diagnosis of carcinoid tumours and the investigation of the effects of drugs on the neurological system.
  • Clonality

    Monoclonal
  • Clone number

    YC5/45
  • Isotype

    IgG2c
  • Light chain type

    kappa
  • Research areas

    • Neuroscience
    • Neurotransmitter
    • Biogenic Amines
    • Serotonin / 5HT

Associated products

  • Compatible Secondaries

    • Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
    • Goat Anti-Rat IgG H&L (HRP) (ab205720)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab6336 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr (1)
Use a concentration of 1 - 5 µg/ml. (see Ni et al). Fix with ice cold acetone. Dilute antibody in 1.5% blocking serum.
IHC-P (1)
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
IHC-FoFr
1/100 - 1/1000. Used on gelatin embedded perfusion fixed tissue (see recommended protocol).
Notes
IHC-Fr
Use a concentration of 1 - 5 µg/ml. (see Ni et al). Fix with ice cold acetone. Dilute antibody in 1.5% blocking serum.
IHC-P
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
IHC-FoFr
1/100 - 1/1000. Used on gelatin embedded perfusion fixed tissue (see recommended protocol).

Target

  • Relevance

    Serotonin (5-hydroxytryptamine, or 5-HT) is a monoamine neurotransmitter synthesised in serotonergic neurons in the central nervous system and enterochromaffin cells in the gastrointestinal tract. Serotonin plays an important part in the biochemistry of depression, migraine, bipolar disorder and anxiety. It is also believed to be influential on sexuality and appetite. 5-HT is generally thought not to be released from synaptic terminal buttons in the manner of classical neurotransmission but from serotonergic varacosities into the extra neuronal space. From here it is free to diffuse over a relatively large region of space (>20µm) and activate 5-HT receptors located on the dendrites, cell bodies and presynaptic terminals of adjacent neurons. Serotonergic action is terminated primarily via uptake of 5-HT from the synapse. This is through the specific monoamine transporter for 5-HT, 5-HT reuptake transporter, on the presynaptic neuron. The pharmacology of 5-HT is extremely complex, with its actions being mediated by a large and diverse range of 5-HT receptors.
  • Cellular localization

    Cytoplasmic and Secreted
  • Alternative names

    • 5 hydroxytryptamine antibody
    • 5HT antibody

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serotonin antibody [YC5/45] (ab6336)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serotonin antibody [YC5/45] (ab6336)

    ab6336 in rat brain tissue fixed with 4% paraformaldehyde. The staining is appropriate, lighting up cell bodies, dendrites and terminals of known 5-HT containing neurones. These pictures are from the review provided by Jim Deuchars.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serotonin antibody [YC5/45] (ab6336)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serotonin antibody [YC5/45] (ab6336)
    IHC image of Serotonin staining in mouse brain (coronal) formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab6336, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • Immunohistochemistry (Frozen sections) - Anti-Serotonin antibody [YC5/45] (ab6336)
    Immunohistochemistry (Frozen sections) - Anti-Serotonin antibody [YC5/45] (ab6336)This image is courtesy of Ms. Szu-yu Liu by an abreview

    Paraformaldehyde-fixed rabbit spinal cord tissue stained for Serotonin using ab6336 at 1/200 dilution in immunohistochemical analysis. Power Block™ Universal Blocking Reagen t(Biogenex) was used as ablocking agent for 10 minute(s) at a concentration of 10% at 37°C. Biotin-SP-conjugated Goat Anti-Rat IgG was used as the secondary antibody at 1/500 dilution.

    See Abreview

Protocols

  • IHC on gelatin embedded perfusion fixed tissue

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (15)

    Publishing research using ab6336? Please let us know so that we can cite the reference in this datasheet.

    ab6336 has been referenced in 15 publications.

    • Zhang W  et al. Wheel Running Improves Motor Function and Spinal Cord Plasticity in Mice With Genetic Absence of the Corticospinal Tract. Front Cell Neurosci 13:106 (2019). PubMed: 30941019
    • Humenick A  et al. Characterization of projections of longitudinal muscle motor neurons in human colon. Neurogastroenterol Motil 31:e13685 (2019). PubMed: 31355986
    • König D  et al. Distribution and Restoration of Serotonin-Immunoreactive Paraneuronal Cells During Caudal Fin Regeneration in Zebrafish. Front Mol Neurosci 12:227 (2019). PubMed: 31616250
    • Sitaraman D  et al. Discrete Serotonin Systems Mediate Memory Enhancement and Escape Latencies after Unpredicted Aversive Experience inDrosophilaPlace Memory. Front Syst Neurosci 11:92 (2017). PubMed: 29321732
    • Dhillon RS  et al. Axonal plasticity underpins the functional recovery following surgical decompression in a rat model of cervical spondylotic myelopathy. Acta Neuropathol Commun 4:89 (2016). IHC-Fr . PubMed: 27552807
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-8 of 8 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Serotonin antibody [YC5/45]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (colon)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM Citrate buffer pH 6
    Permeabilization
    Yes - TritonX100 0.5%
    Specification
    colon
    Blocking step
    Dako Blocking solution as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
    Fixative
    Formalin 4%
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 16 2020

    Immunohistochemistry (Frozen sections) abreview for Anti-Serotonin antibody [YC5/45]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rabbit Tissue sections (spinal cord)
    Specification
    spinal cord
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    Power Block™ Universal Blocking Reagent(Biogenex) as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 37°C
    Read More

    Ms. Szu-yu Liu

    Verified customer

    Submitted Nov 23 2012

    Question

    Product code: 6336
    Inquiry: Dear abcam technical support team: This customer would like to order ab6336 (Anti-Serotonin antibody [YC5/45]) antibody) and test in IHC-Fr with rabbit sample, would you please help this customer to create a discount code, the information of this customer as follow: xxxxxxx
    Could you please help this customer to solve this problem? Thanks for your kindly help. Best regards

    Read More

    Abcam community

    Verified customer

    Asked on Jun 27 2012

    Answer

    Thank you for contacting us.

    The anitbody ab6336 has been tested in IHC-Fr but as yet has not been used with rabbit samples. This is therefore information that we would be very interested in. I have therefore generated the discount code as requested which is stated below:

    Discount code: xxxxxxx

    Expiration date: 24th October 2012

    If you have any further questions please do not hesitate to ask.

    Read More

    Abcam Scientific Support

    Answered on Jun 27 2012

    Question

    I'm interested in your rat anti-serotonin monoclonal antibody (YC5/45); code ab6336. However It is in the culture supernant form. Could you tell me what is the concentration of the antibody? Thanks! Have a nice holiday!

    Read More

    Abcam community

    Verified customer

    Asked on Dec 19 2003

    Answer

    Thank you for your enquiry. Ab6336 is in an unpurified form, tissue culture supernatant, and we cannnot guarantee the concentration for unpurified material.

    Read More

    Abcam Scientific Support

    Answered on Dec 19 2003

    Question

    Do you have antibody to serotonin transporter for cats?

    Read More

    Abcam community

    Verified customer

    Asked on Nov 07 2003

    Answer

    Thank you for your enquiry. I did a search in our online catalog and the following antibodies are the serotonin antibodies which we currently have available: ab3439, ab3440, ab6444, ab3505, ab3506, ab6336, ab8882, ab2403, and ab949. Unfortunately, none of them have yet been tested for cross-reactivity in cats. Should you decide to go ahead and purchase any of these products, please let us know how you get on and in return we will forward a reward of your choice, typically an Amazon gift voucher. If you have any more questions, please contact us again.

    Read More

    Abcam Scientific Support

    Answered on Nov 07 2003

    Question

    Antibody requested: 5HT2B, 5HT2C, 5HT1B (1) are these clones of Serotonin? (2) what company has them available?

    Read More

    Abcam community

    Verified customer

    Asked on Oct 13 2003

    Answer

    We produce some of our antibodies in-house and others are sourced from suppliers whose products we believe will be of interest to our customers. The identity of our suppliers remains commercially sensitive information.

    Read More

    Asdf Edo

    Abcam Scientific Support

    Answered on Oct 13 2003

    Question

    I have tested this antibody on formaldehyde fixed Xenopus laevis whole embryos (stages 28 to 34) and I have not detected a reliable signal. I used concentrations of 1:1, 1:10 and 1:20. Do you have any information on the use of this antibody with Xenopus samples?

    Read More

    Abcam community

    Verified customer

    Asked on Jun 21 2001

    Answer

    The serotonin antibody was tested on mature frog tissue.

    Read More

    Abcam Scientific Support

    Answered on Jun 21 2001

    Question

    I would like to lable serotinergic cells in chicken brain on cryosections (perhaps Paraffin) with fluorescence. Can you recomand this antibody for this purpose?

    Read More

    Abcam community

    Verified customer

    Asked on Jan 29 2001

    Answer

    So far, this antibody has not been tested on chicken samples.

    Read More

    Abcam Scientific Support

    Answered on Jan 30 2001

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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