Overview

  • Product name
    Anti-Serotonin antibody [YC5/45]
    See all Serotonin primary antibodies
  • Description
    Rat monoclonal [YC5/45] to Serotonin
  • Host species
    Rat
  • Tested applications
    Suitable for: IHC-P, IHC-Fr, ICC/IF, IHC-FoFrmore details
  • Immunogen

    5HT-bovine seroalbumin.

  • Positive control
    • In IHC, this antibody gave a positive signal in a formalin-fixed, paraffin-embedded coronal section of Mouse Brain.
  • General notes


    Useful for the diagnosis of carcinoid tumours and the investigation of the effects of drugs on the neurological system.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Purity
    Tissue culture supernatant
  • Primary antibody notes
    Useful for the diagnosis of carcinoid tumours and the investigation of the effects of drugs on the neurological system.
  • Clonality
    Monoclonal
  • Clone number
    YC5/45
  • Isotype
    IgG2c
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6336 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. PubMed: 22474435
IHC-Fr Use a concentration of 1 - 5 µg/ml. (see Ni et al). Fix with ice cold acetone. Dilute antibody in 1.5% blocking serum.
ICC/IF Use at an assay dependent concentration.
IHC-FoFr 1/100 - 1/1000. Used on gelatin embedded perfusion fixed tissue (see recommended protocol).

Target

  • Relevance
    Serotonin (5-hydroxytryptamine, or 5-HT) is a monoamine neurotransmitter synthesised in serotonergic neurons in the central nervous system and enterochromaffin cells in the gastrointestinal tract. Serotonin plays an important part in the biochemistry of depression, migraine, bipolar disorder and anxiety. It is also believed to be influential on sexuality and appetite. 5-HT is generally thought not to be released from synaptic terminal buttons in the manner of classical neurotransmission but from serotonergic varacosities into the extra neuronal space. From here it is free to diffuse over a relatively large region of space (>20µm) and activate 5-HT receptors located on the dendrites, cell bodies and presynaptic terminals of adjacent neurons. Serotonergic action is terminated primarily via uptake of 5-HT from the synapse. This is through the specific monoamine transporter for 5-HT, 5-HT reuptake transporter, on the presynaptic neuron. The pharmacology of 5-HT is extremely complex, with its actions being mediated by a large and diverse range of 5-HT receptors.
  • Cellular localization
    Cytoplasmic and Secreted
  • Alternative names
    • 5 hydroxytryptamine antibody
    • 5HT antibody

Images

  • ab6336 in rat brain tissue fixed with 4% paraformaldehyde. The staining is appropriate, lighting up cell bodies, dendrites and terminals of known 5-HT containing neurones. These pictures are from the review provided by Jim Deuchars.

  • IHC image of Serotonin staining in mouse brain (coronal) formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab6336, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • Paraformaldehyde-fixed rabbit spinal cord tissue stained for Serotonin using ab6336 at 1/200 dilution in immunohistochemical analysis. Power Block™ Universal Blocking Reagen t(Biogenex) was used as ablocking agent for 10 minute(s) at a concentration of 10% at 37°C. Biotin-SP-conjugated Goat Anti-Rat IgG was used as the secondary antibody at 1/500 dilution.

    See Abreview

References

This product has been referenced in:
  • Sitaraman D  et al. Discrete Serotonin Systems Mediate Memory Enhancement and Escape Latencies after Unpredicted Aversive Experience inDrosophilaPlace Memory. Front Syst Neurosci 11:92 (2017). Read more (PubMed: 29321732) »
  • Dhillon RS  et al. Axonal plasticity underpins the functional recovery following surgical decompression in a rat model of cervical spondylotic myelopathy. Acta Neuropathol Commun 4:89 (2016). IHC-Fr . Read more (PubMed: 27552807) »
See all 11 Publications for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Rabbit Tissue sections (spinal cord)
Specification
spinal cord
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Power Block™ Universal Blocking Reagent(Biogenex) as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 37°C

Ms. Szu-yu Liu

Verified customer

Submitted Nov 23 2012

Answer

Thank you for contacting us.

The anitbody ab6336 has been tested in IHC-Fr but as yet has not been used with rabbit samples. This is therefore information that we would be very interested in. I have therefore generated the discount code as requested which is stated below:

Discount code: xxxxxxx

Expiration date: 24th October 2012

If you have any further questions please do not hesitate to ask.

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Answer

Thank you for your enquiry. Ab6336 is in an unpurified form, tissue culture supernatant, and we cannnot guarantee the concentration for unpurified material.

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Question
Answer

Thank you for your enquiry. I did a search in our online catalog and the following antibodies are the serotonin antibodies which we currently have available: ab3439, ab3440, ab6444, ab3505, ab3506, ab6336, ab8882, ab2403, and ab949. Unfortunately, none of them have yet been tested for cross-reactivity in cats. Should you decide to go ahead and purchase any of these products, please let us know how you get on and in return we will forward a reward of your choice, typically an Amazon gift voucher. If you have any more questions, please contact us again.

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Answer

We produce some of our antibodies in-house and others are sourced from suppliers whose products we believe will be of interest to our customers. The identity of our suppliers remains commercially sensitive information.

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Answer

So far, this antibody has not been tested on chicken samples.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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