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Useful for the diagnosis of carcinoid tumours and the investigation of the effects of drugs on the neurological system.
Our Abpromise guarantee covers the use of ab6336 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. PubMed: 22474435|
|IHC-Fr||Use a concentration of 1 - 5 µg/ml. (see Ni et al). Fix with ice cold acetone. Dilute antibody in 1.5% blocking serum.|
|ICC/IF||Use at an assay dependent concentration.|
|IHC-FoFr||1/100 - 1/1000. Used on gelatin embedded perfusion fixed tissue (see recommended protocol).|
ab6336 in rat brain tissue fixed with 4% paraformaldehyde. The staining is appropriate, lighting up cell bodies, dendrites and terminals of known 5-HT containing neurones. These pictures are from the review provided by Jim Deuchars.
Paraformaldehyde-fixed rabbit spinal cord tissue stained for Serotonin using ab6336 at 1/200 dilution in immunohistochemical analysis. Power Block™ Universal Blocking Reagen t(Biogenex) was used as ablocking agent for 10 minute(s) at a concentration of 10% at 37°C. Biotin-SP-conjugated Goat Anti-Rat IgG was used as the secondary antibody at 1/500 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"