Anti-Serotonin antibody [YC5/45] (ab6336)
Key features and details
- Rat monoclonal [YC5/45] to Serotonin
- Suitable for: IHC-Fr, IHC-P
- Reacts with: Species independent
- Isotype: IgG2c
Overview
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Product name
Anti-Serotonin antibody [YC5/45]
See all Serotonin primary antibodies -
Description
Rat monoclonal [YC5/45] to Serotonin -
Host species
Rat -
Tested applications
Suitable for: IHC-Fr, IHC-Pmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to Serotonin conjugated to bovine serum albumin.
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Positive control
- In IHC, this antibody gave a positive signal in a formalin-fixed, paraffin-embedded coronal section of Mouse Brain.
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General notes
Useful for the diagnosis of carcinoid tumours and the investigation of the effects of drugs on the neurological system.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.05% Thimerosal (merthiolate) -
Concentration information loading...
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Purity
Tissue culture supernatant -
Primary antibody notes
Useful for the diagnosis of carcinoid tumours and the investigation of the effects of drugs on the neurological system. -
Clonality
Monoclonal -
Clone number
YC5/45 -
Isotype
IgG2c -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6336 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr | (2) |
Use at an assay dependent concentration.
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IHC-P | (1) |
Use at an assay dependent concentration.
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Notes |
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IHC-Fr
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
Target
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Relevance
Serotonin (5-hydroxytryptamine, or 5-HT) is a monoamine neurotransmitter synthesised in serotonergic neurons in the central nervous system and enterochromaffin cells in the gastrointestinal tract. Serotonin plays an important part in the biochemistry of depression, migraine, bipolar disorder and anxiety. It is also believed to be influential on sexuality and appetite. 5-HT is generally thought not to be released from synaptic terminal buttons in the manner of classical neurotransmission but from serotonergic varacosities into the extra neuronal space. From here it is free to diffuse over a relatively large region of space (>20µm) and activate 5-HT receptors located on the dendrites, cell bodies and presynaptic terminals of adjacent neurons. Serotonergic action is terminated primarily via uptake of 5-HT from the synapse. This is through the specific monoamine transporter for 5-HT, 5-HT reuptake transporter, on the presynaptic neuron. The pharmacology of 5-HT is extremely complex, with its actions being mediated by a large and diverse range of 5-HT receptors. -
Cellular localization
Cytoplasmic and Secreted -
Alternative names
- 5 hydroxytryptamine antibody
- 5HT antibody
Images
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ab6336 in rat brain tissue fixed with 4% paraformaldehyde. The staining is appropriate, lighting up cell bodies, dendrites and terminals of known 5-HT containing neurones. These pictures are from the review provided by Jim Deuchars.
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IHC image of Serotonin staining in mouse brain (coronal) formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab6336, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Paraformaldehyde-fixed rabbit spinal cord tissue stained for Serotonin using ab6336 at 1/200 dilution in immunohistochemical analysis. Power Block™ Universal Blocking Reagen t(Biogenex) was used as ablocking agent for 10 minute(s) at a concentration of 10% at 37°C. Biotin-SP-conjugated Goat Anti-Rat IgG was used as the secondary antibody at 1/500 dilution.
Datasheets and documents
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SDS download
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Datasheet download
References (24)
ab6336 has been referenced in 24 publications.
- Zhang S et al. Occlusal Disharmony-A Potential Factor Promoting Depression in a Rat Model. Brain Sci 12:N/A (2022). PubMed: 35741632
- Li B et al. Engineered 5-HT producing gut probiotic improves gastrointestinal motility and behavior disorder. Front Cell Infect Microbiol 12:1013952 (2022). PubMed: 36339343
- Liu H et al. Blunted diurnal firing in lateral habenula projections to dorsal raphe nucleus and delayed photoentrainment in stress-susceptible mice. PLoS Biol 19:e3000709 (2021). PubMed: 33690628
- Fields AM et al. Vitamin B6 deficiency disrupts serotonin signaling in pancreatic islets and induces gestational diabetes in mice. Commun Biol 4:421 (2021). PubMed: 33772108
- Norkin M et al. High-content, targeted RNA-seq screening in organoids for drug discovery in colorectal cancer. Cell Rep 35:109026 (2021). PubMed: 33882314