Overview

  • Product name
    Serotonin ELISA Kit
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    346.1 ng/ml 20 4.2%
    53.8 ng/ml 20 5.8%
    13.5 ng/ml 20 11%
    Inter-assay
    Sample n Mean SD CV%
    358.0 ng/ml 16.2%
    53.9 ng/ml 18.4%
    13.2 ng/ml 12.7%
  • Sample type
    Urine, Serum, Plasma, Platelets
  • Assay type
    Competitive
  • Sensitivity
    0.293 ng/ml
  • Range
    0.49 ng/ml - 500 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Urine 101 5ng/ml - 100ng/ml
    Serum 91 5ng/ml - 100ng/ml
    Platelets 107 5ng/ml - 100ng/ml
    Citrate Plasma 95 5ng/ml - 100ng/ml

  • Assay time
    3h 00m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: a wide range of other species
  • Product overview

    Serotonin ELISA kit is a competitive ELISA kit designed for the accurate quantitative measurement of Serotonin in Human Serum, Platelets, Plasma and Urine.


    A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated- Serotonin antigen and a polyclonal rabbit antibody specific to Serotonin. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of Serotonin captured in the plate.

  • Notes

    Serotonin (5-hydroxytryptamine, 5-HT) is a monoamine found in the central nervous system, gastrointestinal tract, and blood with broad physiological functions as a neurotransmitter, in gastric motility, hemostasis, and cardiovascular integrity. Defects in serotonin signaling have been linked to a large number of complex behavioral disorders in humans, including anxiety and depression, autism, and eating disorders, and to neurodegenerative disorders such as Parkinson’s. Platelets serve as the major reservoir of serotonin in the bloodstream. When activated, platelets release serotonin into the bloodstream where it acts as a powerful vasoconstrictor. Treatment with Selective Serotonin Uptake Inhibitors (SSRIs) dramatically reduces platelet serotonin concentrations, and altered levels of serotonin in the circulatory system are implicated in such diverse conditions such as asthma, liver cirrhosis and carcinoid tumors.

    phosphatase on the serotonin conjugate. Stop solution is added. The yellow color is read at 405nm. The amount of signal is indirectly proportional to the amount of serotonin in the sample.

     

    Cross reactivities:

     

    Compound Cross Reactivity
    N-Acetyl Serotonin 17%
    5-Hydroxy-L-tryptophan 0.4%
    Tryptamine 0.1%
    5-Hydroxyindoleacetic acid 0.03%
    Melatonin 0.01%
    Tyramine <0.004%
    Tryptophan <0.004%
  • Tested applications
    Suitable for: Competitive ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab133053 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

Images

  • Serotonin measured (averages of undil., 1:3, 1:9 & 1:27) in biological fluids showing quantity (ng) per mL of tested sample.

  • Representative Standard Curve using ab133053.

  • Dose-response curves from Human platelets and Human serum diluted into assay buffer were compared to the Serotonin standard curve. The parallel response indicates the standard effectively mimics the native protein.

Protocols

References

This product has been referenced in:
  • Reddy KE  et al. Effects of High Levels of Deoxynivalenol and Zearalenone on Growth Performance, and Hematological and Immunological Parameters in Pigs. Toxins (Basel) 10:N/A (2018). Read more (PubMed: 29518941) »
  • Yisireyili M  et al. Angiotensin receptor blocker irbesartan reduces stress-induced intestinal inflammation via AT1a signaling and ACE2-dependent mechanism in mice. Brain Behav Immun 69:167-179 (2018). Read more (PubMed: 29155324) »
See all 3 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Objective: quantitative measurement of serotonin in the cerebrospinal fluid (CSF) of patients with Parkinson's disease who underwent deep brain stimulation.
To assess the quality of the samples we performed prior quantification of total protein samples of cerebrospinal fluid through the Pierce's method.
It was possible to obtain levels around 0.30 ug / ul of total protein per sample. We use Serotonin Abcam kit for the detection of serotonin in the CSF samples and all controls and the standard curve functioned according to the specifications of the kit.
It was not possible to detect serotonin in the samples of these patients, suggesting that this level of serotonin in the cerebrospinal fluid is smaller than the detection capability of this kit.

Abcam user community

Verified customer

Submitted Jun 14 2016

Abreviews
We have successfully used this ELISA kit to quantify serotonin levels in the frontal cortex of mice. 1mm thick sections of fresh tissue was extracted, kept on ice, and homogenised mechanically on the same day in 3ml of PBS. The calibration curve was easy to calculate manually on an Excel sheet and yielded excellent results, with both intra and inter-assay Coefficient of Variations below 30%. The one downside of this product is that little Assay Buffer is provided, thus limiting the possibility of multiple serial dilutions for every sample.

Abcam user community

Verified customer

Submitted Mar 16 2016

Abreviews
We have used the ELISA kit and found that it works well on rat tissue lysates, CSF, and in blood samples. The only real draw back was that sometimes high amounts of protein are required, and can sometimes be problematic when working with small isolated brain regions, such as the the hippocampus or CSF samples, which have dilute protein concentrations.

John Karaian

Verified customer

Submitted Dec 03 2015

Serotonin Elisa Kit with rat sample

Average Good 4/5 (Ease of Use)
Abreviews
I used the serotonin elisa kit with rat serum samples of tbi, ptsd, and control treatment groups. The kit gave decent results but I will say that the serotonin levels for my samples were significantly higher than the standard curve of the kit and also the standard error within treatment group was high. I would suggest anyone using this kit with rat serum samples should dilute the samples in half with the assay buffer. One thing that surprised me about this kit is that it calls for 100uL of serum/sample and they want you to do the samples in duplicate which is an enormous amount of serum in my opinion. On a positive note the directions were clear and easy to follow however you want to start this kit early in the day as there are two incubation steps that total three hours. On a side note the kit contains total activity, nonspecific binding, and maximum binding wells which were hard for me to label on my older plate reader which had none of these options when I went to set up my plate in the plate reader protocol. This may not be a problem for newer plate readers that were created for these more complicated elisa kits that require a 4-parameter logistic curve fit to determine concentration.

Brandi Benford

Verified customer

Submitted Mar 03 2014

Answer

Thank you for contacting us.


The kit was not specifically validated for cell culture supernatants but it is possible they may work for mouse assuming that there is enough for the kit to detect. Plus, Serotonin is not species specific (only samples containing endogenous
rabbit IgG would cause an issue). You would need to of course optimize dilution and be able to demonstrate dilutional linearity while falling onto the curve with the cell culture supernatant.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for contacting us.

The reason most kits are validated for fluids is because this marker is primarily expected to be in circulation/extracellular; therefore, the source of the kit informed me that they have not tested brain tissue let alone any other intracellular sample types.
As long as there is enough for the kit to detect it may work but sample handling/dilution optimization will be a necessary requirement of any customer especially when working with a non-validated sample type.

I am sorry I cannot offer any additional specific information. Please do not hesitate to contact us if you need any more advice or information.

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