We have successfully used this ELISA kit to quantify serotonin levels in the frontal cortex of mice. 1mm thick sections of fresh tissue was extracted, kept on ice, and homogenised mechanically on the same day in 3ml of PBS. The calibration curve was easy to calculate manually on an Excel sheet and yielded excellent results, with both intra and inter-assay Coefficient of Variations below 30%. The one downside of this product is that little Assay Buffer is provided, thus limiting the possibility of multiple serial dilutions for every sample.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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Submitted Mar 16 2016