Objective: quantitative measurement of serotonin in the cerebrospinal fluid (CSF) of patients with Parkinson's disease who underwent deep brain stimulation.
To assess the quality of the samples we performed prior quantification of total protein samples of cerebrospinal fluid through the Pierce's method.
It was possible to obtain levels around 0.30 ug / ul of total protein per sample. We use Serotonin Abcam kit for the detection of serotonin in the CSF samples and all controls and the standard curve functioned according to the specifications of the kit.
It was not possible to detect serotonin in the samples of these patients, suggesting that this level of serotonin in the cerebrospinal fluid is smaller than the detection capability of this kit.
To assess the quality of the samples we performed prior quantification of total protein samples of cerebrospinal fluid through the Pierce's method.
It was possible to obtain levels around 0.30 ug / ul of total protein per sample. We use Serotonin Abcam kit for the detection of serotonin in the CSF samples and all controls and the standard curve functioned according to the specifications of the kit.
It was not possible to detect serotonin in the samples of these patients, suggesting that this level of serotonin in the cerebrospinal fluid is smaller than the detection capability of this kit.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
Submitted Jun 14 2016